Rapid and precise retinotopic mapping of the visual cortex obtained by voltage-sensitive dye imaging in the behaving monkey.

Abstract:

:Retinotopy is a fundamental organizing principle of the visual cortex. Over the years, a variety of techniques have been used to examine it. None of these techniques, however, provides a way to rapidly characterize retinotopy, at the submillimeter range, in alert, behaving subjects. Voltage-sensitive dye imaging (VSDI) can be used to monitor neuronal population activity at high spatial and temporal resolutions. Here we present a VSDI protocol for rapid and precise retinotopic mapping in the behaving monkey. Two monkeys performed a fixation task while thin visual stimuli swept periodically at a high speed in one of two possible directions through a small region of visual space. Because visual space is represented systematically across the cortical surface, each moving stimulus produced a traveling wave of activity in the cortex that could be precisely measured with VSDI. The time at which the peak of the traveling wave reached each location in the cortex linked this location with its retinotopic representation. We obtained detailed retinotopic maps from a region of about 1 cm(2) over the dorsal portion of areas V1 and V2. Retinotopy obtained during <4 min of imaging had a spatial precision of 0.11-0.19 mm, was consistent across experiments, and reliably predicted the locations of the response to small localized stimuli. The ability to rapidly obtain precise retinotopic maps in behaving monkeys opens the door for detailed analysis of the relationship between spatiotemporal dynamics of population responses in the visual cortex and perceptually guided behavior.

journal_name

J Neurophysiol

authors

Yang Z,Heeger DJ,Seidemann E

doi

10.1152/jn.00417.2007

subject

Has Abstract

pub_date

2007-08-01 00:00:00

pages

1002-14

issue

2

eissn

0022-3077

issn

1522-1598

pii

00417.2007

journal_volume

98

pub_type

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