Abstract:
:The effect of Ca2+ and calmodulin has been studied on adenylate cyclase activity in homogenates of rat islets of Langerhans. EGTA had a stimulatory effect on the enzyme in accord with the known inhibitory effect of Ca2+. In contrast, the addition of Ca2+ together with calmodulin is stimulatory and demonstrates the existence of a Ca2+-dependent adenylate cyclase in islets of Langerhans. It is suggested that the glucose-induced increase in cyclic AMP concentrations in intact islets is a secondary consequence of the glucose-induced increase in cytosol free-Ca2+ concentrations which, with calmodulin, causes an increase in the activity of adenylate cyclase.
journal_name
Diabetesjournal_title
Diabetesauthors
Sharp GW,Wiedenkeller DE,Kaelin D,Siegel EG,Wollheim CBdoi
10.2337/diab.29.1.74subject
Has Abstractpub_date
1980-01-01 00:00:00pages
74-7issue
1eissn
0012-1797issn
1939-327Xjournal_volume
29pub_type
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