Abstract:
:Exposure of isolated rat pancreatic islets to 1 mM 3-isobutyl-1-methyl-xanthine (IBMX) resulted in a 5.7-fold increase in the rate of insulin release. After a 30-min rest period under basal conditions, reexposure of the islets to 1 mM IBMX resulted in an enhanced rate of release when compared with either a timed control -islets simultaneously exposed to IBMX for the first time-or with the first priming response to IBMX. The possibility of a continuous priming effect was suggested by the gradually rising rate of insulin release during exposure to IBMX. It is concluded that islets have a memory for IBMX exposure that results in increased responsiveness to subsequent stimulation by IBMX.
journal_name
Diabetesjournal_title
Diabetesauthors
Wiedenkeller DE,Sharp GWdoi
10.2337/diab.30.9.754subject
Has Abstractpub_date
1981-09-01 00:00:00pages
754-6issue
9eissn
0012-1797issn
1939-327Xjournal_volume
30pub_type
杂志文章相关文献
DIABETES文献大全abstract::A corpus of evidence suggests that T-helper type 1 (Th1)-dependent cellular immunity plays a pivotal role in the pathogenesis of autoimmune diabetes. This study was intended to find ways to prevent the development of NOD diabetes using a neutralizing anti-interleukin (IL)-12 antibody (C17.8) that inhibits Thl cell dif...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.49.12.1998
更新日期:2000-12-01 00:00:00
abstract::Mesenchymal stem cells (MSCs) possess immunoregulatory, anti-inflammatory, and proangiogenic properties and, therefore, have the potential to improve islet engraftment and survival. We assessed the effect human bone marrow-derived MSCs have on neonatal porcine islets (NPIs) in vitro and determined islet engraftment an...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-1068
更新日期:2017-05-01 00:00:00
abstract::Proinsulin is converted to insulin by the concerted action of two sequence-specific subtilisin-like proteases termed prohormone convertase 2 (PC2) and prohormone convertase 3 (PC3). PC3 is a type I proinsulin-processing enzyme that initiates the sequential processing of proinsulin to insulin by cleaving the proinsulin...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.45.7.897
更新日期:1996-07-01 00:00:00
abstract::Alterations in dopaminergic circuitry play a critical role in food reward and may contribute to susceptibility to obesity. Ingestion of sweets releases dopamine in striatum, and both sweet preferences and striatal D2 receptors (D2R) decline with age and may be altered in obesity. Understanding the relationships betwee...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-0407
更新日期:2016-09-01 00:00:00
abstract::Low-grade sustained inflammation links obesity to insulin resistance and nonalcoholic fatty liver disease (NAFLD). However, therapeutic approaches to improve systemic energy balance and chronic inflammation in obesity are limited. Pharmacological activation of nuclear factor (erythroid-derived 2)-like 2 (Nrf2) allevia...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-0662
更新日期:2017-05-01 00:00:00
abstract::Nonketotic, genetically diabetic Cinese hamsters show subnormal pancreatic insulin release and impaired suppression of glucagon in response to glucose. To study the pancreatic effects of other agents, dynamic insulin and glucagon release was measured from the in vitro perfused pancreases of normal and diabetic Chinese...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.24.3.273
更新日期:1975-03-01 00:00:00
abstract::Chronic hyperglycemia desensitizes beta-cells to glucose. To further define the mechanisms behind desensitization and the role of overstimulation, we tested human pancreatic islets for the effects of long-term elevated glucose levels on cytoplasmic free Ca2+ concentration ([Ca2+]i) and its relationship to overstimulat...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.49.11.1840
更新日期:2000-11-01 00:00:00
abstract::Alterations in renal function and structure are found even at the onset of diabetes mellitus. Studies performed over the last decade now allow definition of a series of stages in the development of renal changes in diabetes. Such a classification may be useful both in clinical work and in research activities. Stage 1 ...
journal_title:Diabetes
pub_type: 杂志文章,评审
doi:10.2337/diab.32.2.s64
更新日期:1983-05-01 00:00:00
abstract::Increased abdominal obesity has been related to lower insulin sensitivity (SI), independent of overall obesity, but it has been suggested that this relationship may be weaker in non-whites. In the Insulin Resistance and Atherosclerosis Study (IRAS), SI was estimated using a minimal model analysis of the frequently sam...
journal_title:Diabetes
pub_type: 杂志文章,多中心研究
doi:10.2337/diab.45.11.1547
更新日期:1996-11-01 00:00:00
abstract:OBJECTIVE:To investigate if recurrent autoimmunity explained hyperglycemia and C-peptide loss in three immunosuppressed simultaneous pancreas-kidney (SPK) transplant recipients. RESEARCH DESIGN AND METHODS:We monitored autoantibodies and autoreactive T-cells (using tetramers) and performed biopsy. The function of auto...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db09-0498
更新日期:2010-04-01 00:00:00
abstract:OBJECTIVE:Haptoglobin (Hp) is upregulated in both inflammation and obesity. The low chronic inflammatory state, caused by massive adipose tissue macrophage (ATM) infiltration found in obesity, and low adiponectin have been implicated in the development of insulin resistance and hepatosteatosis. The aim of this work was...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db10-1536
更新日期:2011-10-01 00:00:00
abstract::Ciglitazone, 5-[4-(1-methylcyclohexylmethoxy) benzyl]-thiazolidine-2,4-dione, is a new hypoglycemic agent orally active in the obese-hyperglycemic animal models. In C57BL/6J-ob/ob mice, treatment with 100 mg/kg ciglitazone for 2 days elicited a drastic fall in blood glucose. It also decreased plasma insulin, triglycer...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.9.830
更新日期:1983-09-01 00:00:00
abstract::In vivo voltammetry was used to measure the synaptic release of rat striatal dopamine and serotonin after the administration of the amino acid L-tryptophan to streptozocin-induced diabetic rats. Dopamine and serotonin release from rat striatum was studied at a short-term or acute (3-day) interval and a long-term or ch...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.7.956
更新日期:1988-07-01 00:00:00
abstract::NO has been identified recently as the prime islet-toxic product of inflammatory macrophages. The adverse effects of IL-1 on isolated islets also have been reported to involve NO. We now show that exposure of an islet cell suspension to the NO donor nitroprusside or to activated macrophages leads to DNA strand breaks....
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.42.3.496
更新日期:1993-03-01 00:00:00
abstract::Endothelial cells in culture retain many of the functional properties of the endothelium in vivo. At high cell density, they become contact-inhibited. Endothelial cells, like vascular smooth muscle cells and fibroblasts, express binding sites for low density lipoprotein when depleted of sterol. In contact-inhibited en...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.30.2.s19
更新日期:1981-01-01 00:00:00
abstract::Filtered proteins including insulin are absorbed in the proximal tubule by means of pinocytosis. The first step in this process is binding of the protein to brush border membrane. As it is not known whether absorption exhibits specificity, we set out to determine whether specific binding sites for insulin are present ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.31.7.618
更新日期:1982-07-01 00:00:00
abstract:OBJECTIVE:The aim of the current study was to evaluate the long-term effects of leptin on glucose metabolism, diabetes complications, and life span in an insulin-dependent diabetes model, the Akita mouse. RESEARCH DESIGN AND METHODS:We cross-mated Akita mice with leptin-expressing transgenic (LepTg) mice to produce Ak...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db10-1795
更新日期:2011-09-01 00:00:00
abstract::Congenital malformations occur more frequently in the offspring of diabetic mothers. These in vivo and in vitro studies investigate the potential adverse effects of hyperglycemia on kidney development in the rat. Female rats were made hyperglycemic throughout gestation with a single injection of streptozotocin (STZ) o...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diabetes.48.11.2240
更新日期:1999-11-01 00:00:00
abstract::The kinetics of in vivo insulin-mediated glucose uptake in human obesity have not been previously studied. To examine this, we used the glucose-clamp technique to measure whole-body and leg muscle glucose uptake in seven lean and six obese men during hyperinsulinemia (approximately 2000 pM) at four glucose levels (app...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.39.8.965
更新日期:1990-08-01 00:00:00
abstract::Effects of epinephrine (Epi) infusion on the absorption of subcutaneously injected 125I-labeled soluble human insulin (10 U) from the thigh or the abdomen were studied in 16 healthy subjects and from the thigh in 10 insulin-dependent diabetic (IDDM) patients. Epi was infused at 0.3 (high dose) or 0.1 (low dose; health...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.37.6.694
更新日期:1988-06-01 00:00:00
abstract::Sera from rats inoculated with in vitro glucosylated rat skin acid-soluble collagen were evaluated for specific antibody response using the enzyme-linked immunosorbent assay. The data indicate that the altered protein is capable of eliciting the production of antibodies, but unmodified collagen is not. The specificity...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.32.12.1182
更新日期:1983-12-01 00:00:00
abstract::Hepatocyte growth factor (HGF) is a mitogen required for β-cell replication during pregnancy. To determine whether HGF/c-Met signaling is required for β-cell regeneration, we characterized mice with pancreatic deletion of the HGF receptor, c-Met (PancMet KO mice), in two models of reduced β-cell mass and regeneration:...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db13-0333
更新日期:2014-01-01 00:00:00
abstract::The effect of 4 wk of streptozocin (STZ)-induced diabetes on the transmission time of the auditory-evoked brain stem response (ABR) was examined in conscious male Sprague-Dawley rats. Distal nerve transmission time of the auditory pathway (latency of peak II), which includes conduction along the 8th cranial nerve, inc...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.36.4.500
更新日期:1987-04-01 00:00:00
abstract::Diabetic hypoglycemia produces cognitive-motor slowing, which is assumed to increase risk of automobile crashes. This study investigated driving decrements during and after hypoglycemia, and the patients' awareness of driving decrements. We used a randomized, single-blind, crossover design and conducted the study at t...
journal_title:Diabetes
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.2337/diab.42.2.239
更新日期:1993-02-01 00:00:00
abstract::The hyperinsulinemia of obese rodents has been associated with a reduced number of hepatic insulin receptors except in hepatocytes from fatty Zucker rats. We isolated liver plasma membranes from 10-11-wk-old lean and fatty Zucker rats, some of which were injected with streptozotocin 2--4 wk earlier. We have determined...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.31.10.867
更新日期:1982-10-01 00:00:00
abstract::Because skeletal muscle plays a major role in glucose disposal, it may be the primary site of insulin resistance in non-insulin-dependent diabetes mellitus (NIDDM). Rates of glycogen synthesis (GS), glucose utilization via glycolysis, glycolytic utilization (GU), and glucose transport (GT) were studied in epitrochlear...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.39.9.1106
更新日期:1990-09-01 00:00:00
abstract::Tolbutamide (1 g/kg body wt) was administered to male rats for 3 days to determine the effects of this pretreatment on subsequent insulin biosynthesis and compartmental storage characteristics of freshly isolated islets. Islets were isolated 16 h after the last tolbutamide administration, at a time when fed plasma glu...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/diab.35.1.6
更新日期:1986-01-01 00:00:00
abstract::The relationships between diabetes and pancreatic ductal adenocarcinoma (PDAC) are complex. Longstanding type 2 diabetes (T2DM) is a risk factor for pancreatic cancer, but increasing epidemiological data point to PDAC as also a cause of diabetes due to unknown mechanisms. New-onset diabetes is of particular interest t...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-1477
更新日期:2017-05-01 00:00:00
abstract::Hypoxia-inducible factor (HIF)-1 mediates hypoxia- and chronic kidney disease-induced fibrotic events. Here, we assessed whether HIF-1 blockade attenuates the manifestations of diabetic nephropathy in a type 1 diabetic animal model, OVE26. YC-1 [3-(5'-hydroxymethyl-2'-furyl)-1-benzyl indazole], an HIF-1 inhibitor, red...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db15-0519
更新日期:2016-05-01 00:00:00
abstract::We explored the role of reactive oxygen species (ROS) in the pathogenesis of muscle insulin resistance. We assessed insulin action in vivo with a hyperinsulinemic-euglycemic clamp in mice expressing a mitochondrial-targeted catalase (MCAT) that were fed regular chow (RC) or a high-fat diet (HFD) or underwent an acute ...
journal_title:Diabetes
pub_type: 杂志文章
doi:10.2337/db16-1334
更新日期:2017-08-01 00:00:00