Abstract：
:The levels of rabbit alpha-foetoprotein (RAFP) in foetal serum, neonatal serum and amniotic fluid during gestation have been evaluated by quantitative radial immunodiffusion. RAFP attained its peak concentration on the 24th day of pregnancy in foetal serum, while in amniotic fluid peaks were observed on ;the 14th and 26th day. Temporal effects on the heterogeneity of RAFP were analysed by concanavalin A affinity chromatography and agarose electrophoresis. The proportion of concanavalin A non-reactive RAFP decreased during pregnancy in foetal serum, amniotic fluid and yolk sac extracts and these findings were confirmed by crossed affino-immunoelectrophoresis. On ordinary crossed immunoelectrophoresis 14-day foetal serum migrated as a single peak, but by 24 days a second peak was evident. No similar change was detected in amniotic fluid, yolk sac or liver extract. The findings are compared with those from similar studies on other species and a model is proposed to explain the observed heterogeneity of RAFP.
journal_name
J Reprod Immunoljournal_title
Journal of reproductive immunologyauthors
Clarke LEdoi
10.1016/0165-0378(80)90026-1subject
Has Abstractpub_date
1980-11-01 00:00:00pages
199-211issue
4eissn
0165-0378issn
1872-7603pii
0165-0378(80)90026-1journal_volume
2pub_type
杂志文章abstract：:During syngeneic pregnancy the numbers of 'spontaneously' occurring ('background') Ig-secreting cells were determined in the spleen, bone marrow (BM) and mesenteric lymph nodes (MLN) of BALB/c mice that were kept under germfree conditions and fed a low molecular weight synthetic diet (GF-CD), SPF BALB/c mice fed autoc...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(86)90017-3
更新日期：1986-11-01 00:00:00
abstract：:Herpes simplex virus type 2 (HSV-2) is becoming increasingly prevalent worldwide, despite the widespread use of antiviral drugs. Its ability to evade the immune system and establish a latent infection has made it difficult to develop an effective vaccine. Our understanding of the immune response against HSV-2 remains ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2011.01.001
更新日期：2011-03-01 00:00:00
abstract：:The NOD mouse is a model for autoimmune diabetes that develops symptoms similar to Type I diabetes. The incidence of diabetes is greater in females but the degree of insulitis is comparable in both sexes. The purpose of this study was to assess the populations of lymphocytes and macrophages in the pancreas and spleen ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(96)00986-2
更新日期：1996-11-01 00:00:00
abstract：:Monocytes are short-lived cells, arising from the bone marrow and maturing in the circulation. They play an important role in immune responses and are thought to be important for healthy pregnancy. In humans, 3 subpopulations of monocytes have been identified: classical, intermediate and non-classical monocytes. These...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2016.06.009
更新日期：2017-02-01 00:00:00
abstract：:Anticardiolipin antibodies (aCL) were eluted from the placentae of four women with elevated serum levels of aCL, demonstrating that these antibodies are bound to affected placentae. Anticardiolipin antibodies bound to affected placentae were only of the IgG isotype and the level of aCL in placental eluates did not ref...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(93)90064-o
更新日期：1993-12-01 00:00:00
abstract：:The feasibility of producing epitope-specific antigens by mutation of the gene is demonstrated, the aim being to eliminate unwanted surface epitopes yet allowing the natural folding of the protein to maintain the desired epitope(s). The model protein is the beta subunit of human chorionic gonadotropin (hCG beta) which...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(96)00978-3
更新日期：1996-08-01 00:00:00
abstract：:The objective of this study was the phenotypic and functional evaluation of decidual immune cells in the cynomolgus and vervet monkeys. Early pregnancy (days 36-42) deciduas were obtained by fetectomy for histological evaluation and decidual mononuclear leukocyte (MNL) isolation. While peripheral NK (pNK) cells in the...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2008.12.006
更新日期：2009-06-01 00:00:00
abstract：:Recent discoveries suggest that T-regulatory lymphocytes (Treg) might play an important role in the pathophysiology of preterm labor. The aim of this study was to assess the relationship among the levels of maternal circulating Treg cells, uterine cervical length, and the risk of preterm labor. Sixty women with regula...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2014.04.001
更新日期：2014-12-01 00:00:00
abstract：:The isolation and partial characterization of an immunosuppressive early pregnancy factor (EPF) present in the serum of pregnant women, between 3 and 8 weeks of gestation, is described. EPF was purified using ion-exchange chromatography, affinity chromatography and HPLC gel permeation techniques. A homogeneous, active...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(86)90018-5
更新日期：1986-11-01 00:00:00
abstract：:HLA-G is a non-classical MHC class 1 molecule, expressed primarily on human foetal trophoblast cells, which exhibits almost no genetic polymorphism. Because of these unusual features, HLA-G has been suggested to help prevent maternal immune attack of the semi-allogeneic foetus. The aim of these experiments was to inve...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(00)00070-x
更新日期：2000-08-01 00:00:00
abstract：:Lipopolysaccharide-binding protein (LBP) is an acute phase protein known to play a central role in the defense against Gram-negative bacteria. It binds lipopolysaccharides of Gram-negative bacteria and, after binding to CD14, the complex signals through Toll-like receptor (TLR)-4, eliciting host-defense responses, suc...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2005.01.005
更新日期：2005-06-01 00:00:00
abstract：:Previous studies have shown that the polymeric Ig receptor (pIgR) is produced by rat uterine epithelial cells both in vivo and vitro. The expression of the pIgR is regulated by sex hormones and/or cytokines at mucosal sites, however the mechanism of regulation in the uterus is not clear. In these studies, co-culture o...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(97)00015-6
更新日期：1997-06-01 00:00:00
abstract：:Alloreactive T levels of para-aortic lymph nodes (PALN) and spleen were determined on different days of pregnancy in BALB/c females by local and systemic graft-versus-host (GvH) assays. A significant increase in GvH reactivity was registered early in both allogeneic and syngeneic matings, operating not only towards pa...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(85)90036-1
更新日期：1985-11-01 00:00:00
abstract：:Although antisperm autoantibody responses to obstruction of the male reproductive system have been documented, information on the nature of the cognate sperm autoantigens has been limited. In the present study, the patterns of sperm autoantigens recognized by sera from rats after obstruction of the vas deferens or epi...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/s0165-0378(98)00090-4
更新日期：1999-05-01 00:00:00
abstract：:Recently developed synthetic Mimetic affinity ligands were screened for their ability to purify estradiol antibody from sheep serum. One of these ligands (color coded Yellow 1) effectively separated immunoglobulin from other serum protein by negative affinity. When 1 ml antiserum diluted 70% (protein content approxima...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(93)90008-6
更新日期：1993-03-01 00:00:00
abstract：:Establishment of pregnancy initiates a dynamic and predictable series of changes in the uterus. In rodents, the trophectoderm of the blastocyst develops through the stage of an ectoplacental cone to become the placenta. The inner cell mass becomes the fetus and its associated extra-embryonic ectoderm and mesoderm. Mat...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/s0165-0378(02)00005-0
更新日期：2002-10-01 00:00:00
abstract：OBJECTIVE:To express the hCGbeta-C3d3 fusion protein in a CHO cell continual expression system to investigate further the adjuvant effects of C3d on contraceptive vaccination. METHOD:We constructed a plasmid pcDNA3-hCGbeta-C3d3 which contains three copies of murine C3d cDNA and the hCGbeta gene by cloning the chimeric...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2003.09.001
更新日期：2003-12-01 00:00:00
abstract：:Purified midgestation murine trophoblast cannot be killed by a variety of cell-mediated effector mechanisms, with the exception of highly lytic effectors such as lymphokine-activated killer cells. We now report that this trophoblast population is also resistant to tumor necrosis factor (TNF)-alpha. ...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(90)90043-6
更新日期：1990-03-01 00:00:00
abstract：:The regulation of uterine and circulating peripheral blood natural killer (NK) cells has been associated with reproductive conditions including recurrent pregnancy loss (RPL), implantation failure and preeclampsia. Natural cytotoxicity receptors (NCRs) are unique markers that regulate NK cell cytotoxicity and cytokine...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2011.04.006
更新日期：2011-06-01 00:00:00
abstract：:A high resolution two dimensional electrophoretic technique (2-D), isoelectric focusing followed by polyacrylamide gel electrophoresis, for separation of neutral and acidic human spermatozoal proteins was established. Silver stained 2-D gels revealed over 260 proteins with Mr 20-200 kDa and pI between 4.5 and 7.8. Mem...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(90)90001-m
更新日期：1990-06-01 00:00:00
abstract：:Pregnancy is thought to be a state of immunological tolerance. The mechanisms underlying this phenomenon are still poorly understood. In our previous study, adenovirus mediated CTLA4Ig transgene (Ad-CTLA4Ig) therapy was demonstrated to improve pregnancy outcome in an abortion-prone mouse model by skewing the Th2/Th1 c...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2013.01.002
更新日期：2013-04-01 00:00:00
abstract：:Human trophoblast membrane antigens recognised by monoclonal antibodies (H310, H315, H316 and H317) have been identified using combinations of radioimmunoprecipitation, SDS-PAGE, electroblotting, chromatographic and ELISA-type techniques. H317 is known to identify heat-stable placental-type alkaline phosphatase and ac...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(83)90244-9
更新日期：1983-11-01 00:00:00
abstract：:Decidual stromal cells (DSCs) are an important component of decidual tissues where they are in strict proximity with immune cells. Although previous research has indicated that DSCs participate in the regulation of immune cells during pregnancy, the crosstalk between DSCs and decidual NK cells (dNKs) has not been full...
journal_title：Journal of reproductive immunology
pub_type： 临床试验,杂志文章
doi：10.1016/j.jri.2015.01.004
更新日期：2015-06-01 00:00:00
abstract：:Autophagy is an evolutionarily conserved process in eukaryotes to maintain cellular homeostasis against stress. This process has two main functions: producing energy and quality control of intracellular proteins. During early pregnancy, extravillous trophoblasts (EVTs) invade the uterine myometrium and migrate along t...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2017.08.006
更新日期：2017-09-01 00:00:00
abstract：:It has been suggested that obesity and loss of ovarian function alter the inflammatory response to immune stress. Ovariectomized (OVX) rats, which are used as a model of human menopause, exhibit both hyperphagia-induced obesity and gonadal steroid deficiency. To evaluate the effects of ovariectomy on inflammatory resp...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2011.05.010
更新日期：2011-09-01 00:00:00
abstract：:Throughout follicular growth the number of immune cells increases, enhanced under stimulation with exogenous gonadotropins. This treatment, however, may adversely influence folliculogenesis and negatively affect oocyte quality through modifications in the follicular concentrations of cytokines released by these immune...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/j.jri.2014.06.003
更新日期：2014-12-01 00:00:00
abstract：:Antisera to Mason-Pfizer or Baboon Endogenous Virus possessed complement-dependent cytotoxicity for cell lines chronically infected with these viruses, with some degree of cross-reaction. When appropriately absorbed with virus-free cells, the antisera were not cytotoxic for lymphocytes of adult males but lysed lymphoc...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(81)90001-2
更新日期：1981-03-01 00:00:00
abstract：:A method has been developed to establish lines of transformed lymphocytes able to produce in vitro the same anti-sperm antibodies as those naturally occurring in immuno-infertile individuals. We utilized lymphocytes from a male donor whose serum contained anti-sperm antibodies of the IgG class up to the dilution 1:10,...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(95)00939-i
更新日期：1995-07-01 00:00:00
abstract：:Members of the galectin family are expressed within the female reproductive tract and have been shown to be involved in multiple biological functions that support the progression of pregnancy. Specific expression patterns of different members of this family have been identified at the maternal decidua and on the place...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章,评审
doi：10.1016/j.jri.2013.05.005
更新日期：2014-03-01 00:00:00
abstract：:This study examines the distribution of two carbohydrate determinants recognized by monoclonal antibodies EMA-1 and EMA-6 on cells in culture, adult mouse tissues and day 0-7 embryos, and provides partial biochemical characterization of the molecules carrying the determinants for EMA-1 on embryonal carcinoma cells. Bo...
journal_title：Journal of reproductive immunology
pub_type： 杂志文章
doi：10.1016/0165-0378(87)90069-6
更新日期：1987-02-01 00:00:00
