Enzyme-substrate reactions in very high magnetic fields. I.

abstract：:We use molecular dynamics simulations to characterize the influence of cholesterol (Chol) on the interaction between the anticancer drug doxorubicin (DOX) and a dipalmitoyl phosphatidylcholine/Chol lipid bilayer. We calculate the potential of mean force, which gives us an estimate of the free energy barrier for DOX tr...

journal_title：Biophysical journal

authors： Yacoub TJ,Reddy AS,Szleifer I

更新日期：2011-07-20 00:00:00

abstract：:The equation of motion for sperm flagella, in which the elastic bending moment and the active contractile moment are balanced by the moment from the viscous resistance of the surrounding fluid, is solved for a wave solution that superimposes partial solutions. Substitution of the expression for the wave solution into ...

journal_title：Biophysical journal

authors： Rikmenspoel R

更新日期：1978-08-01 00:00:00

abstract：:Molecular dynamics (MD) simulations of 5 ns on protein-DNA complexes of catabolite-activator protein (CAP), lambda-repressor, and their corresponding uncomplexed protein and DNA, are reported. These cases represent two extremes of DNA bending, with CAP DNA bent severely and the lambda-operator nearly straight when com...

journal_title：Biophysical journal

authors： Dixit SB,Andrews DQ,Beveridge DL

更新日期：2005-05-01 00:00:00

abstract：:Amyloid fibrils are associated with many neurodegenerative diseases. It was found that amyloidogenic oligomers, not mature fibrils, are neurotoxic agents related to these diseases. Molecular mechanisms of infectivity, pathways of aggregation, and molecular structure of these oligomers remain elusive. Here, we use all-...

journal_title：Biophysical journal

authors： Blinov N,Dorosh L,Wishart D,Kovalenko A

更新日期：2010-01-20 00:00:00

abstract：:Experiments have shown that the ability of the HIV-1 virus to infect cells can be greatly diminished by deactivation of the N-terminal (fusion) peptide of its glycoprotein gp41. Deactivation can be achieved by the deletion of several amino acid residues, or replacement of a hydrophobic residue with a polar residue, to...

journal_title：Biophysical journal

authors： Maddox MW,Longo ML

更新日期：2002-12-01 00:00:00

abstract：:Synchrotron x-ray diffraction, differential scanning calorimetry, and electron spin resonance spectroscopy have been employed to characterize a quasicrystalline phase formed in aqueous dispersions of binary mixtures of glucocerebroside and palmitoyloleoylphosphatidylethanolamine. Small- and wide-angle x-ray scattering...

journal_title：Biophysical journal

authors： Feng Y,Rainteau D,Chachaty C,Yu ZW,Wolf C,Quinn PJ

更新日期：2004-04-01 00:00:00

abstract：:One of the most common swimming strategies employed by microorganisms is based on the use of rotating helical filaments, called flagella, that are powered by molecular motors. Determining the physical properties of this propulsive system is crucial to understanding the behavior of these organisms. Furthermore, the abi...

journal_title：Biophysical journal

authors： Pierce CJ,Osborne E,Mumper E,Lower BH,Lower SK,Sooryakumar R

更新日期：2019-10-01 00:00:00

abstract：:Molecular chaperones are large proteins or protein complexes from which many proteins require assistance in order to fold. One unique property of molecular chaperones is the cavity they provide in which proteins fold. The interior surface residues which make up the cavities of molecular chaperone complexes from differ...

journal_title：Biophysical journal

authors： White AD,Huang W,Jiang S

更新日期：2012-12-19 00:00:00

abstract：:Cubic insulin crystals contain a binding site for monovalent cations in a cavity of the crystal dyad in which the bound cation is ligated by protein atomic dipoles and water molecules. These types of interaction are analogous to interactions that occur in small cation-selective carrier and channel molecules. X-ray dif...

journal_title：Biophysical journal

authors： Badger J,Kapulsky A,Gursky O,Bhyravbhatla B,Caspar DL

更新日期：1994-02-01 00:00:00

abstract：:Inactivation is a fundamental property of voltage-gated ion channels. Fast inactivation of Na(+) channels involves channel block by the III-IV cytoplasmic interdomain linker. The mechanisms of nonfast types of inactivation (intermediate, slow, and ultraslow) are unclear, although the ionic environment and P-loops rear...

journal_title：Biophysical journal

authors： Tikhonov DB,Zhorov BS

更新日期：2007-09-01 00:00:00

abstract：:39K nuclear magnetic resonance (NMR) spectra were readily obtained, in vivo, from rat muscle, kidney, and brain in 5-10 min with signal-to-noise ratios of approximately 20:1. Quantitation of the K+ signal was achieved by reference to an external standard of KCl/dysprosium nitrate as well as by reference to the proton ...

journal_title：Biophysical journal

authors： Adam WR,Koretsky AP,Weiner MW

更新日期：1987-02-01 00:00:00

abstract：:Rearrangement of the actin cytoskeleton is integral to cell shape and function. Actin-binding proteins, e.g., filamin, can naturally contribute to the mechanics and function of the actin cytoskeleton. The molecular mechanical bases for filamin's function in actin cytoskeletal reorganization are examined here using mol...

journal_title：Biophysical journal

authors： Kolahi KS,Mofrad MR

更新日期：2008-02-01 00:00:00

abstract：:Actin is one of the main components of the architecture of cells. Actin filaments form different polymer networks with versatile mechanical properties that depend on their spatial organization and the presence of cross-linkers. Here, we investigate the mechanical properties of actin bundles in the absence of cross-lin...

journal_title：Biophysical journal

authors： Rückerl F,Lenz M,Betz T,Manzi J,Martiel JL,Safouane M,Paterski-Boujemaa R,Blanchoin L,Sykes C

更新日期：2017-09-05 00:00:00

abstract：:Five AHCAPs exhibiting a broad-spectrum of antimicrobial activity, were examined with regard to their action in lipid mixtures with two anionic lipids, PG and CL. We find that all of the peptides studied were capable of promoting the formation of crystalline phases of DMPG in mixtures of DMPG and CL, without prior inc...

journal_title：Biophysical journal

authors： Epand RF,Maloy L,Ramamoorthy A,Epand RM

更新日期：2010-06-02 00:00:00

abstract：:Most of the decisive molecular events in biology take place at the protein-water interface. The dynamical properties of the hydration layer are therefore of fundamental importance. To characterize the dynamical heterogeneity and rotational activation energy in the hydration layer, we measured the (17)O spin relaxation...

journal_title：Biophysical journal

authors： Mattea C,Qvist J,Halle B

更新日期：2008-09-15 00:00:00

abstract：:Understanding the fundamentals of genome packaging in viral capsids is important for finding effective antiviral strategies and for utilizing benign viral particles for gene therapy. While the structure of encapsidated genomic materials has been routinely characterized with experimental techniques such as cryo-electro...

journal_title：Biophysical journal

authors： Kim J,Wu J

更新日期：2015-10-20 00:00:00

abstract：:Cellular membranes are laterally organized into domains of distinct structures and compositions by the differential interaction affinities between various membrane lipids and proteins. A prominent example of such structures are lipid rafts, which are ordered, tightly packed domains that have been widely implicated in ...

journal_title：Biophysical journal

authors： Lin X,Gorfe AA,Levental I

更新日期：2018-04-24 00:00:00

abstract：:Starzak and Starzak (1978. IEEE Trans. Biomed. Eng. 25:201-204.) proposed that, in cyclic application of current and voltage-clamps, the fidelity of the match of the output current with the original stimulus could be used to measure the spatial uniformity of voltage in a membrane. However, they failed to find such a m...

journal_title：Biophysical journal

authors： Moore JW,Stockbridge N,Schiff SJ

更新日期：1982-12-01 00:00:00

abstract：:Several biologically important protein structures give rise to strong second-harmonic generation (SHG) in their native context. In addition to high-contrast optical sections of cells and tissues, SHG imaging can provide detailed structural information based on the physical constraints of the optical effect. In this st...

journal_title：Biophysical journal

authors： Plotnikov SV,Millard AC,Campagnola PJ,Mohler WA

更新日期：2006-01-15 00:00:00

abstract：:To assay cell cycle progression in synchronized culture of yeast we have applied dielectric spectroscopy to its real-time monitoring. The dielectric monitoring is based on the electromagnetic induction method, regarded as a nonelectrode method, which has resolved the problems encountered in measurements with metal ele...

journal_title：Biophysical journal

authors： Asami K,Gheorghiu E,Yonezawa T

更新日期：1999-06-01 00:00:00

abstract：:The TRPV1 cation nonselective ion channel plays an essential role in thermosensation and perception of other noxious stimuli. TRPV1 can be activated by low extracellular pH, high temperature, or naturally occurring pungent molecules such as allicin, capsaicin, or resiniferatoxin. Its noxious thermal sensitivity makes ...

journal_title：Biophysical journal

authors： Ladrón-de-Guevara E,Dominguez L,Rangel-Yescas GE,Fernández-Velasco DA,Torres-Larios A,Rosenbaum T,Islas LD

更新日期：2020-02-25 00:00:00

abstract：:We have studied the photoinduced volume changes, energetics, and kinetics in the early steps of the bacteriorhodopsin (BR) photocycle with pulsed, time-resolved photoacoustics. Our data show that there are two volume changes. The fast volume change ( < or = 200 ns) is an expansion (2.5 +/- 0.3 A3/molecule) and is obse...

journal_title：Biophysical journal

authors： Zhang D,Mauzerall D

更新日期：1996-07-01 00:00:00

abstract：:Several investigators have demonstrated that under conditions where little or no gas exchange occurs across the alveolar capillary membrane the PCO(2) is higher in the alveolus than in the mixed venous blood, whereas there are no PO(2) differences. Gurtner et al. have explained the DeltaPCO(2) by a model in which H(+)...

journal_title：Biophysical journal

authors： Gurtner GH

更新日期：1972-06-01 00:00:00

abstract：:Fusion is a crucial event in the infection of animal cells by enveloped viruses (e.g., HIV or influenza). Viral fusion is mediated by glycoproteins, spanning the viral envelope, which attach to a membrane surface and induce fusion of the viral envelope to the cellular membrane. Influenza fusion protein (hemagglutinin)...

journal_title：Biophysical journal

authors： Longo ML,Waring AJ,Hammer DA

更新日期：1997-09-01 00:00:00

abstract：:Recent experimental studies of the role of microtubule sliding in neurite outgrowth suggested a qualitative model, according to which kinesin-1 motors push the minus-end-out microtubules against the cell membrane and generate the early cell processes. At the later stage, dynein takes over the sliding, expels the minus...

journal_title：Biophysical journal

authors： Oelz DB,Del Castillo U,Gelfand VI,Mogilner A

更新日期：2018-10-16 00:00:00

abstract：:Spectroscopic responses of the potentiometric probe 2-(4-(dimethylamino)styryl)-1-methylpyridinium iodide (DASPMI) were investigated in living cells by means of a time- and space-correlated single photon counting technique. Spatially resolved fluorescence decays from single mitochondria or only a very few organelles o...

journal_title：Biophysical journal

authors： Ramadass R,Bereiter-Hahn J

更新日期：2008-10-01 00:00:00

abstract：:Despite the recognition that actin filaments are important for numerous cellular processes, and decades of investigation, the dynamics of in vitro actin filaments are still not completely understood. Here, we follow the time evolution of the length distribution of labeled actin reporter filaments in an unlabeled F-act...

journal_title：Biophysical journal

authors： Schmoller KM,Niedermayer T,Zensen C,Wurm C,Bausch AR

更新日期：2011-08-17 00:00:00

abstract：:Glasses used to fabricate patch pipettes may release components which affect ion channels (Cota, G., and C.M. Armstrong. 1988. Biophys. J. 53:107-109; Furman, R.E., and J.C. Tanaka. 1988. Biophys. J. 53:287-292; Rojas, L., and C. Zuazaga. 1988. Neurosci. Lett. 88:39-44). The gating properties of maxi K+ channels from ...

journal_title：Biophysical journal

authors： Copello J,Simon B,Segal Y,Wehner F,Ramanujam VM,Alcock N,Reuss L

更新日期：1991-10-01 00:00:00

abstract：:Cell migration is based on an actin treadmill, which in turn depends on recycling of G-actin across the cell, from the rear where F-actin disassembles, to the front, where F-actin polymerizes. To analyze the rates of the actin transport, we used the Virtual Cell software to solve the diffusion-drift-reaction equations...

journal_title：Biophysical journal

authors： Novak IL,Slepchenko BM,Mogilner A

更新日期：2008-08-01 00:00:00

abstract：:The time correlation function of the shape fluctuations of large (greater than 10 micron), cylindrical, hydrated, phospholipid-membrane vesicles consisting of one bimolecular layer was measured. The restoring force of the membrane was due to the excess curvature of a membrane element. A value for the curvature elastic...

journal_title：Biophysical journal

authors： Schneider MB,Jenkins JT,Webb WW

更新日期：1984-05-01 00:00:00