Abstract:
:Despite the recognition that actin filaments are important for numerous cellular processes, and decades of investigation, the dynamics of in vitro actin filaments are still not completely understood. Here, we follow the time evolution of the length distribution of labeled actin reporter filaments in an unlabeled F-actin solution via fluorescence microscopy. Whereas treadmilling and diffusive length fluctuations cannot account for the observed dynamics, our results suggest that at low salt conditions, spontaneous fragmentation is crucial.
journal_name
Biophys Jjournal_title
Biophysical journalauthors
Schmoller KM,Niedermayer T,Zensen C,Wurm C,Bausch ARdoi
10.1016/j.bpj.2011.07.009subject
Has Abstractpub_date
2011-08-17 00:00:00pages
803-8issue
4eissn
0006-3495issn
1542-0086pii
S0006-3495(11)00838-1journal_volume
101pub_type
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