Fragmentation is crucial for the steady-state dynamics of actin filaments.

Abstract:

:Despite the recognition that actin filaments are important for numerous cellular processes, and decades of investigation, the dynamics of in vitro actin filaments are still not completely understood. Here, we follow the time evolution of the length distribution of labeled actin reporter filaments in an unlabeled F-actin solution via fluorescence microscopy. Whereas treadmilling and diffusive length fluctuations cannot account for the observed dynamics, our results suggest that at low salt conditions, spontaneous fragmentation is crucial.

journal_name

Biophys J

journal_title

Biophysical journal

authors

Schmoller KM,Niedermayer T,Zensen C,Wurm C,Bausch AR

doi

10.1016/j.bpj.2011.07.009

subject

Has Abstract

pub_date

2011-08-17 00:00:00

pages

803-8

issue

4

eissn

0006-3495

issn

1542-0086

pii

S0006-3495(11)00838-1

journal_volume

101

pub_type

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