Abstract：
:Differential precipitation of plasma proteins after equilibration of the plasma with tracer amounts of testosterone (T) was used to measure free and not-T-estradiol binding globulin (TeBG)-bound T in 20 women and 10 men. Tritiated T was the tracer used. Comparison of this method with the equilibration dialysis method produced a good agreement in accuracy. The method is described as simple and reproducible, allowing routine assay of 20 plasma samples in duplicate by 1 technician. Normal values for men were 497 ng/100 ml (total T), 42 ng/100 ml(free T), 137 ng/100 ml (non-TeBG bound), and .5 mcg/100 ml (TeBG). Women's values were 52 ng/100 ml (Total T), 2.6 ng/100 ml (free T), 5 ng/100 ml (nonTeBG) and .9 mcg/100 ml (TeBG). Significant increases in total (p less than .05), free (p less than .01), non-TeBG-bound (p less than .05), and TeBG-T (p less than .01) were found for women taking norethindrone and mestranol. Women taking norgestrel-ethinyl estradiol had significant increase (p less than .01) in non-TeBG-bound T. It is concluded that estrogens increase the binding capacity of TeBG, and this is inhibited by norgestrel.
journal_name
Contraceptionjournal_title
Contraceptionauthors
Tremblay RR,Dube JYdoi
10.1016/0010-7824(74)90099-7subject
Has Abstractpub_date
1974-12-01 00:00:00pages
599-605issue
6eissn
0010-7824issn
1879-0518pii
0010-7824(74)90099-7journal_volume
10pub_type
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