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Physiology of murine B lymphocytes. II. Life-spans of mitogen and thymus-independent antigen (type 2) reactive B lymphocytes from aged mice.

Abstract：

:We showed earlier that life spans of murine B lymphocytes could be estimated by measuring the functional reactivities of normal B cells upon transfer into x-linked immunodeficient (xid) mice, which do not respond to anti-mouse IgM (anti-mu) antibodies and thymic-independent type-2 (TI-2) antigens. Here the same approach was adopted, to evaluate the life spans of B-lymphocytes from aged mice. Spleen cells from normal young and aged mice were transferred into young or aged xid recipients and the decay kinetics were followed by measuring the proliferative response to anti-mu and PFC response to TNP-Ficoll, a prototype TI-2 antigen. The results indicated that anti-mu reactive B cells of both young and aged mice decayed with similar non-linear kinetics. About 50% of the donor cells decayed in 8-10 days, whereas, the remaining decayed at a slower rate and it appeared that the median life-expectancy of this latter population could be at least 3 weeks. Essentially, there was no apparent difference in the decay kinetics of anti-mu reactive B cells of young and aged mice. Unlike anti-mu reactive cells, TNP-Ficoll reactive B cells showed 2-3-fold enhancement in the PFC response during the first 2 weeks, and persisted at least until 5 weeks post transfer. This result indicated that TNP-Ficoll reactive B cells are long-lived. Further, it was found that the turn over rate of TNP-Ficoll reactive B cells was also very similar in young and aged mice. The environment of the aged mice also did not appear to have any effect since the survival profiles of anti-mu reactive B cells were the same in young or aged xid recipients. Altogether, these results suggest that aging does not significantly alter the life spans of mature B lymphocytes.

journal_name

Mech Ageing Dev

journal_title

Mechanisms of ageing and development

authors

Udhayakumar V,Goud SN,Cross RJ,Subbarao B

doi

10.1016/0047-6374(91)90012-o

subject

Has Abstract

pub_date

1991-12-02 00:00:00

pages

135-47

issue

eissn

0047-6374

issn

1872-6216

pii

0047-6374(91)90012-O

journal_volume

pub_type

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Physiology of murine B lymphocytes. II. Life-spans of mitogen and thymus-independent antigen (type 2) reactive B lymphocytes from aged mice.