Inhibitory activity of the ethyl acetate fraction from Viscum coloratum on bone resorption.

Abstract:

:The effects of four fractions, a hexane fraction, an ethyl acetate fraction, an N-butanol fraction and a water fraction, from a water extract of Herba Visci were investigated to find the fraction, in IN VITRO experiments, with the greatest ability to inhibit the formation of osteoclast-like multinucleated cells from mouse bone marrow cells and also to inhibit (45)Ca release from a mouse parietal bone organ culture system. The ethyl acetate fraction was able to inhibit the formation of osteoclast-like cells in a dose-dependent manner and was also able to potently inhibit the release of (45)Ca even at a low concentration. Therefore, a further IN VIVO experiment was performed to determine if the ethyl acetate fraction had antiosteoporotic activity. It was found to inhibit the decreases in total and cancellous bone mineral density, in cancellous and cortical bone mineral content as well as in cortical bone thickness and in the X-axis strength index of tibiae of ovariectomized rats. The principle constituents of the ethyl acetate fraction were determined to be (+)-syringaresinol O- beta-glucopyranoside, 2-homoeriodictyol 7- O-glucoside and viscumneoside I by HPLC analysis.

journal_name

Planta Med

journal_title

Planta medica

authors

Yin J,Han N,Xu X,Liu Z,Zhang B,Kadota S

doi

10.1055/s-2008-1034283

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

120-5

issue

2

eissn

0032-0943

issn

1439-0221

journal_volume

74

pub_type

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