Abstract:
:A crude globulin fraction was isolated from the tubers of Pinellia ternata (Thunb.) Breit. Tubers of P. ternata are one of the well known Chinese traditional medicines. It is used on account of its anti-emetic effect. 6KDP was separated by means of gel filtration and chromatography on CM-Toyopearl 650 M from the crude globulin fraction. 6KDP is a glycoprotein of M.W. 6 kDa. 6KDP has an anti-emetic activity and hemagglutination activity. Both activities dropped during heat treatment. For the purpose to evaluate the quality of the powdered tubers of P. ternata, the quantitative analysis by means of EIA was established. The contents of 6KDP varied from 5.75 to 8.30%. The assay demonstrated a low cross-reaction with the crude globulin fraction of Colocasia antiquorum, a member of the same family as P. ternata. 6KDP is one of major proteins in the tubers of P. ternata. Thus 6KDP is a marker for quantitative analysis of the tubers of P. ternata.
journal_name
Planta Medjournal_title
Planta medicaauthors
Kurata K,Tai T,Yang Y,Kinoshita K,Koyama K,Takahashi K,Watanabe K,Nunoura Ydoi
10.1055/s-2006-957539subject
Has Abstractpub_date
1998-10-01 00:00:00pages
645-8issue
7eissn
0032-0943issn
1439-0221journal_volume
64pub_type
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