Abstract：
:The influence of the shape of the red blood cell during stomatocyte-echinocyte transformation on its deformability was studied by microsieving through pores with diameters of 2.6, 4.5, and 6.9 micron. A stomatocytic transformation was produced by chlorpromazine (0.02, 0.1, and 0.5 mmol/L) and an echinocytic transformation by sodium salicylate (7.5, 30, and 120 mmol/L). For spherostomatocytes, an increase in filtration resistance through 2.6 and 4.5 micron pores was observed, whereas for spheroechinocytes, a decrease in filtration resistance through 2.6 micron pores was found. Larger pores (6.9 micron) were not sensitive to those shape changes. The changes in deformability can be explained by the fact that the surface area of (sphero)-stomatocytes decreased, whereas that of (sphero)-echinocytes increased; the cell volume remained essentially constant. Echinocytes produced by 24-hour adenosine triphosphate depletion differed from drug-induced echinocytes: they had an increased cell volume at constant surface area and consequently an increased filtration resistance through 2.6- and 4.5-micron filter pores. Shape changes with spicule formation are therefore not a homogeneous entity, and cell geometric factors (eg, surface area and volume) must be assessed with care. The viscosity of red cell suspensions at a hematocrit level of 45% was higher for drug-induced echinocytes than discocytes or stomatocytes at all shear rates tested. We conclude that the normal discocyte represents an optimum shape for the flow in vivo since a stomatocytic transformation could impair the passage through the microcirculation (decrease in cell filterability) and an echinocytic transformation could impair the flow in larger vessels (increase in blood viscosity).
journal_name
Bloodjournal_title
Bloodauthors
Reinhart WH,Chien Ssubject
Has Abstractpub_date
1986-04-01 00:00:00pages
1110-8issue
4eissn
0006-4971issn
1528-0020journal_volume
67pub_type
杂志文章相关文献
BLOOD文献大全abstract：:The chemokines platelet factor 4 (PF4) and RANTES (regulated on activation normal T cell expressed and secreted) are secreted by activated platelets and influence multiple cell types and biologic processes. For instance, PF4 inhibits progenitor cell proliferation and angiogenesis, while platelet-derived RANTES is invo...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2004-06-2475
更新日期：2005-02-01 00:00:00
abstract：:The CD28 family of receptors (CD28, cytotoxic T-lymphocyte-associated antigen 4 [CTLA-4], inducible costimulator [ICOS], program death-1 [PD-1], and B- and T-lymphocyte attenuator [BTLA]) plays a critical role in controlling the adaptive arm of the immune response. While considerable information is available regarding...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2004-04-1596
更新日期：2005-01-01 00:00:00
abstract：:The presence and physiologic role of cyclic GMP-dependent protein kinase (G-kinase) in human neutrophils was investigated by Western blot analysis and immunocytochemistry. Small quantities of G-kinase were found in the cytoskeletal-enriched fraction of neutrophil lysates as detected by Western blots using a polyclonal...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Optimal dose and timing of CHOP (cyclophosphamide, doxorubicin, vincristine, and prednisone) chemotherapy for aggressive non-Hodgkin lymphoma (NHL) is still an unresolved issue. We assessed whether dose intensifications with cyclophosphamide and doxorubicin might improve outcome in younger patients with intermediate-r...
journal_title：Blood
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1182/blood-2006-07-035709
更新日期：2007-04-01 00:00:00
abstract：:The translocation between chromosomes 8 and 21, t(8;21) (q22;q22), is the most frequent abnormality in acute myeloid leukemia (AML) with French-American-British type M2 (FAB-M2) morphology. The breakpoints in this translocation have been characterized at the molecular level, and the genes involved are AML1 on chromoso...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1993-08-01 00:00:00
abstract：:MCL-1 is a Bcl-2 family member that has been described as antiapoptotic in various myeloid neoplasms. Therefore, MCL-1 has been suggested as a potential new therapeutic target. Systemic mastocytosis (SM) is a myeloid neoplasm involving mast cells (MCs) and their progenitors. In the present study, we examined the expre...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-07-032714
更新日期：2007-04-01 00:00:00
abstract：:High-dose melphalan followed by stem cell transplantation (HDM-SCT) has become the treatment of choice for patients with immunoglobulin light chain amyloidosis (AL). Unfortunately, treatment mortality can be excessive in certain subpopulations. We have noted that patients who gained more than 2% body weight during mob...
journal_title：Blood
pub_type： 临床试验,杂志文章
doi：10.1182/blood-2005-03-1206
更新日期：2005-11-15 00:00:00
abstract：:The majority of patients with Hodgkin lymphoma (HL) are cured with initial therapy. However, high-dose therapy with autologous hematopoietic cell transplant (AHCT) allows for the cure of an additional portion of patients with relapsed or primary refractory disease. Positron emission tomography-negative complete remiss...
journal_title：Blood
pub_type： 杂志文章,评审
doi：10.1182/blood-2017-09-772673
更新日期：2018-04-12 00:00:00
abstract：:In an attempt to improve outcomes for patients with acute myeloid leukemia (AML) after allogeneic hematopoietic cell transplantation (HCT), we conducted a phase 1/2 study in which targeted irradiation delivered by 131I-anti-CD45 antibody was combined with targeted busulfan (BU; area-under-curve, 600-900 ng/mL) and cyc...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2005-06-2317
更新日期：2006-03-01 00:00:00
abstract：:Cryoprecipitate has been demonstrated to correct the bleeding abnormality of patients with some congenital (storage pool disease) and acquired (uremia) platelet abnormalities, but the reason for this effect is unknown. We found significant platelet contamination in plasma harvested to prepare fresh frozen plasma and c...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1986-07-01 00:00:00
abstract：:Autosomal recessive STAT1 deficiency is associated with impaired cellular responses to interferons and susceptibility to intracellular bacterial and viral infections. We report here a new form of partial STAT1 deficiency in 2 siblings presenting mycobacterial and viral diseases. Both carried a homozygous missense muta...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-04-280586
更新日期：2010-12-23 00:00:00
abstract：:Stimulators of human erythroid burst-forming units (BFU-E) and multipotential colony-forming cells (CFU-GEMM) can be produced by a number of different cell types. A product of human peripheral blood monocytes, interleukin 1 (IL-1), was evaluated for its ability to stimulate fibroblast cultures to produce stimulators o...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1987-01-01 00:00:00
abstract：:A study of the Bernard-Soulier syndrome in two unrelated families using different polyclonal antibodies in a sensitive immunoblot assay showed residual amounts of platelet membrane glycoprotein (GP) lb in the eight homozygotes, as well as the near-absence of GPlb beta and GPIX. The eight heterozygotes studied showed a...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1988-09-01 00:00:00
abstract：:Asthma is associated with airway remodeling. Evidence of platelet recruitment to the lungs of asthmatics after allergen exposure suggests platelets participate in various aspects of asthma; although their importance is unknown in the context of airway remodeling, their involvement in atherosclerosis is established. St...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-05-1707
更新日期：2004-01-15 00:00:00
abstract：:Regulatory molecules produced by stromal cells are often membrane bound until cleaved by matrix metalloproteinases (MMPs); cleavage can either activate or inactivate regulatory functions. We report here that marrow stromal cells induce the expression of MMP-9 in monocytes. Induction was contact independent and could b...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2006-05-020289
更新日期：2007-01-01 00:00:00
abstract：:Antibody-mediated pure red cell aplasia is a rare but serious event resulting from the induction of neutralizing erythropoietin (Epo)-specific antibodies provoked by treatment with recombinant Epo. Because of the crucial role of CD4 T cells in humoral response, we have quantified the number of Epo-specific CD4 T cells...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2010-04-280875
更新日期：2010-11-25 00:00:00
abstract：:Radioimmunotherapy (RIT) has emerged as an effective treatment for lymphoma, however the underlying mechanisms are poorly understood. We therefore investigated the relative contributions of antibody and targeted radiation to the clearance of tumor in vivo, using 2 different syngeneic murine B-cell lymphoma models. Alt...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2003-06-2037
更新日期：2004-02-15 00:00:00
abstract：:Salivary gland mucosa-associated lymphoid tissue (MALT) type lymphomas are typically indolent B-cell neoplasms that are often associated with Sjogren's syndrome. To better define the cell of origin and evaluate whether antigen receptor stimulation may be playing a role in tumor growth, the Ig heavy and light chain var...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1997-05-01 00:00:00
abstract：:Little is known about the molecular mechanisms controlling primitive hematopoietic stem cells, especially during embryogenesis. Homeobox genes encode a family of transcription factors that have gained increasing attention as master regulators of developmental processes and recently have been implicated in the differen...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1996-04-01 00:00:00
abstract：:Different biologic features have been associated with a more or less aggressive clinical course in chronic lymphocytic leukemia (CLL). In the present study, 20 patients with highly stable CLL observed at a single institution over a period of 10 to 23 years and who never required treatment were extensively characterize...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2002-12-3639
更新日期：2003-08-01 00:00:00
abstract：:NF-kappaB is a transcription factor that controls the expression of a number of genes important for mediating immune and inflammatory responses. In this study, we examined whether bortezomib and PS-1145, each of which inhibits NF-kappaB, could protect mice from lethal graft-versus-host disease (GVHD), which is charact...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2005-05-1820
更新日期：2006-01-15 00:00:00
abstract：:An immunosuppressant Rapamycin (Rap) has been reported to cause G1 arrest by inhibiting p70 S6 kinase and G1 cyclin/cdks kinase activities when added to quiescent cells with mitogens. However, antiproliferative effects of Rap on exponentially growing cells have been poorly investigated. We examined the intracellular e...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1998-01-15 00:00:00
abstract：:Plasmin cleaves fibrin at or near sites involved in platelet recognition and may modulate platelet adhesion and spreading. Using an in vitro system, we have characterized the effects of limited plasmic degradation of polymerized fibrin on platelet adhesion and spreading. As shown by scanning electron microscopy, expos...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1994-08-15 00:00:00
abstract：:Growing evidence indicates antibody-dependent cellular cytotoxicity (ADCC) contributes to the clinical response to monoclonal antibody (mAb) therapy of lymphoma. Recent in vitro analysis suggests C3b can inhibit mAb-induced natural killer (NK)-cell activation and ADCC. Further studies were conducted to assess the effe...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2009-01-200469
更新日期：2009-12-17 00:00:00
abstract：:The mechanical properties of sickle erythrocyte membranes were evaluated in the ektacytometer. When ghosts from the total red blood cell population were examined, the rigidity of the resealed ghosts and their rate of fragmentation by shear stress (t1/2) were normal. However, fractionation on Stractan density gradients...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1990-04-15 00:00:00
abstract：:We established a radiation-induced murine hematopoietic cell line, Y6, that could be induced to differentiate into macrophages by interleukin-6 (IL-6). IL-6 also induced growth inhibition and apoptosis in Y6 cells. Retinoic acid (RA) inhibited such effects of IL-6 on Y6 cells. The inhibitory effect of RA on the effect...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1992-11-01 00:00:00
abstract：:Non-Hodgkin lymphomas (NHLs) are the most common cancer to affect pet dogs. In contrast to the many genes whose mutation contributes to lymphomagenesis in humans, relatively little is known about the acquired genetic alterations that lead to canine B-cell lymphomas (cBCLs). We performed a survey of 84 canine NHL tumor...
journal_title：Blood
pub_type： 杂志文章
doi：10.1182/blood-2014-10-602714
更新日期：2015-02-05 00:00:00
abstract：:We have previously observed an increased frequency of liver failure in human immunodeficiency virus (HIV)-infected hemophiliacs. The purpose of this study was to quantitate hepatitis C virus (HCV) RNA levels in serial samples from HIV-seropositive (HIV+) and HIV-seronegative (HIV-) hemophiliacs before and after HIV se...
journal_title：Blood
pub_type： 杂志文章,多中心研究
doi：
更新日期：1994-08-15 00:00:00
abstract：:The monoclonal antibodies Tab and AP3 are directed, respectively, against GPIIb and GPIIIa, the subunits of the platelet fibrinogen receptor. When added together to platelets, these antibodies prevent adenosine diphosphate (ADP)-induced platelet aggregation, despite normal fibrinogen binding (Newman et al, Blood 69:66...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：1990-10-15 00:00:00
abstract：:In a previous study, we reported that a single injection of cyclophosphamide (CTX) in tumor-bearing mice resulted in tumor eradication when the animals were subsequently injected with tumor-sensitized lymphocytes. Notably, CTX acted by inducing bystander effects on T cells, and the response to the combined CTX/adoptiv...
journal_title：Blood
pub_type： 杂志文章
doi：
更新日期：2000-03-15 00:00:00
