Abstract:
:The monoclonal antibodies Tab and AP3 are directed, respectively, against GPIIb and GPIIIa, the subunits of the platelet fibrinogen receptor. When added together to platelets, these antibodies prevent adenosine diphosphate (ADP)-induced platelet aggregation, despite normal fibrinogen binding (Newman et al, Blood 69:668, 1987). To explore the cellular requirements of aggregation after fibrinogen binding, we used several techniques to study platelets treated with Tab and AP3, then stimulated with ADP. We used scanning and transmission electron microscopy to evaluate platelet morphology, immunolabel-surface replication to determine whether individual GPIIb-IIIa complexes clustered, immunocytochemistry on frozen thin sections to study the subcellular distribution of the integrin GPIIb-IIIa and fibrinogen, and biochemical methods to assess the activation of the platelet cytoskeleton. We found that the treated cells had short, blunted projections instead of normal filopodia. Other morphologic abnormalities, apparent in thin section, were aberrantly placed alpha-granules and microtubules, and a prominent, worm-like, fibrinogen-filled surface-connected canalicular system. Biochemical analysis suggested that such platelets undergo massive actomyosin-controlled membrane flow, which serves to sequester GPIIb-IIIa and makes the platelets refractory to aggregation. We conclude that aggregation requires the formation of long, slender filopodia, probably directed by cytoskeletal rearrangements after activation, and that the transmembrane GPIIb-IIIa complex may play a role in signaling these events.
journal_name
Bloodjournal_title
Bloodauthors
Isenberg WM,Bainton DF,Newman PJsubject
Has Abstractpub_date
1990-10-15 00:00:00pages
1564-71issue
8eissn
0006-4971issn
1528-0020journal_volume
76pub_type
杂志文章相关文献
BLOOD文献大全abstract::Down-regulation of immune responses by regulatory T (Treg) cells is an important mechanism involved in the induction of tolerance to allo-antigens (Ags). Recently, a novel subset of Ag-specific T-cell receptor (TCR)alpha beta+ CD4(-)CD8- (double-negative [DN]) Treg cells has been found to be able to prevent the reject...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2004-07-2583
更新日期:2005-04-01 00:00:00
abstract::MEIS1 is a three-amino acid loop extension class homeodomain-containing homeobox (HOX) cofactor that plays key roles in normal hematopoiesis and leukemogenesis. Expression of Meis1 is rate-limiting in MLL-associated leukemias and potently interacts with Hox and NUP98-HOX genes in leukemic transformation to promote sel...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-06-225573
更新日期:2010-05-20 00:00:00
abstract::Complement plays a key role in host defense, but its dysregulation can cause autologous tissue injury. Complement activation is normally controlled by regulatory proteins, including factor H (FH) in plasma and membrane cofactor protein (MCP) on the cell surface. Mutations in FH and MCP are linked to atypical hemolytic...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2016-07-728253
更新日期:2017-03-02 00:00:00
abstract::Despite increasing use of targeted therapies to treat cancer, anemia remains a common complication of cancer therapy. Physician concerns about the safety of intravenous (IV) iron products and erythropoiesis-stimulating agents (ESAs) have resulted in many patients with cancer receiving no or suboptimal anemia therapy. ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019004017
更新日期:2020-08-13 00:00:00
abstract::Adult T-cell leukemia (ATL) and human T-cell lymphotropic virus type I (HTLV-I)-associated myelopathy/tropical spastic paraparesis (HAM/TSP) are known to be caused by HTLV-I infection. However, current methods used to determine HTLV-I infection do not differentiate between HTLV-I asymptomatic carriers (ACs) and ATL an...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-11-390807
更新日期:2012-03-22 00:00:00
abstract::Erythropoietin (EPO) is the hormone necessary for development of erythrocytes from immature erythroid cells. EPO activates Jun N-terminal kinase (JNK), a member of the mitogen-activated protein kinase (MAPK) family in the EPO-dependent murine erythroid HCD57 cells. Therefore, we tested if JNK activity supported prolif...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-05-1754
更新日期:2004-08-01 00:00:00
abstract::Survivin, a member of the inhibitor of apoptosis protein family, is expressed in almost all types of malignancies, making this protein a useful tool for the development of broadly applicable vaccination therapies. We used a recently identified HLA-A2 binding peptide and dendritic cells (DCs) from healthy donors to ind...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-08-2554
更新日期:2003-07-15 00:00:00
abstract::The three-dimensional tertiary structure of human von Willebrand Factor (vWF) on a hydrophobic surface under aqueous conditions and different shear stress regimes was studied by atomic force microscopy (AFM). vWF was imaged by AFM at molecular level resolution under negligible shear stress, under a local applied shear...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-10-15 00:00:00
abstract::The reported outcome of hematopoietic stem cell transplantation (HSCT) from HLA-mismatched family members has been inconsistent. The object of this study was to evaluate the true impact of HLA-mismatch by using recent data from a homogenous population, excluding HSCT procedures that used graft manipulations, and by co...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-02-0430
更新日期:2003-08-15 00:00:00
abstract::Although preclinical work with rapalogs suggests potential in treatment of multiple myeloma (MM), they have been less successful clinically. These drugs allostearically inhibit the mammalian target of rapamycin kinase primarily curtailing activity of the target of rapamycin complex (TORC)1. To assess if the mammalian ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-05-285726
更新日期:2010-11-25 00:00:00
abstract::Most anticancer chemotherapies are immunosuppressive and induce nonimmunogenic tumor cell death. Bortezomib, a specific inhibitor of 26S proteasome, has shown clinical activity in several human tumors, including myeloma. Here we show that the uptake of human myeloma cells by dendritic cells (DCs) after tumor cell deat...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2006-10-054221
更新日期:2007-06-01 00:00:00
abstract::A longstanding endeavor to define the genetic lesions that drive myeloid malignances has stimulated a period of remarkable discovery. Enabled by technological advances that have sharply decreased the cost of DNA sequencing, the full compendium of common, recurrent somatic mutations in the coding genome of myeloid mali...
journal_title:Blood
pub_type: 杂志文章,评审
doi:10.1182/blood-2013-06-460295
更新日期:2013-11-28 00:00:00
abstract::Hematopoietic fate maps in the developing mouse embryo remain imprecise. Definitive, adult-type hematopoiesis first appears in the fetal liver, then progresses to the spleen and bone marrow. Clonogenic common lymphoid progenitors and clonogenic common myeloid progenitors (CMPs) in adult mouse bone marrow that give ris...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.3.627
更新日期:2001-08-01 00:00:00
abstract::A patient with recurrent pulmonary abscess, weight loss, and alcoholism was found to have extremely high serum vitamin B12 and unsaturated vitamin B12-binding capacity (UBBC) levels. While transcobalamin (TC) II was also increased, most of his UBBC was due to an abnormal binding protein which carried greater than 80% ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1977-06-01 00:00:00
abstract::Hemoglobin Ohio [beta 142 (H20) Ala replaced by Asp] was found in three members of a white family, all of whom showed erythrocytosis. The variant hemoglobin has a high oxygen affinity, a reduced Bohr effect, and diminished cooperativity. The functional abnormalities of Hb Ohio are explained by the proximity of the sub...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::As B-cell chronic lymphocytic leukemia (B-CLL) progresses, malignant cells extravasate and infiltrate lymphoid tissues. Several molecules, including gelatinase B/MMP-9, contribute to these processes. Although mainly a secreted protease, some MMP-9 is present at the B-CLL cell surface and the function, mode of anchorin...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2007-08-109249
更新日期:2008-07-01 00:00:00
abstract::Hepcidin is a 25-amino-acid peptide demonstrated to be the iron regulatory hormone capable of blocking iron absorption from the duodenum and iron release from macrophages. Mutations affecting hepcidin regulators or the hepcidin gene itself cause hemochromatosis, a common genetic disorder. Hepcidin is produced mainly b...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-01-550467
更新日期:2014-06-05 00:00:00
abstract::Both extrinsic and intrinsic mechanisms tightly govern hematopoietic stem cell (HSC) decisions of self-renewal and differentiation. However, transcription factors that can selectively regulate HSC self-renewal division after stress remain to be identified. Slug is an evolutionarily conserved zinc-finger transcription ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-07-232934
更新日期:2010-03-04 00:00:00
abstract::Lipid rafts are sphingolipid- and cholesterol-rich membrane microdomains that are insoluble in nonionic detergents, have a low buoyant density, and preferentially contain lipid-modified proteins, like glycosyl phosphatidylinositol (GPI)-anchored proteins. The lipid rafts were isolated from human erythrocytes and major...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.4.1141
更新日期:2001-02-15 00:00:00
abstract::Exposure to ambient particulate matter (PM) air pollution has been reported to trigger inflammation and thrombosis. However, molecular mechanisms underlying the modulation of coagulation pathways in PM-induced thrombosis remain largely unknown. We report here that Sirt1, a member of class III histone deacetylase, cont...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2011-04-350413
更新日期:2012-03-08 00:00:00
abstract::Under normal conditions, platelets do not adhere to endothelium. However, when platelets or endothelial cells are stimulated by thrombin or cytokines, respectively, platelets bind avidly to endothelium. Because there is accumulating evidence that endothelial cells may become apoptotic under certain proinflammatory or ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1999-06-01 00:00:00
abstract::The endothelium, as the interface between blood and all tissues, plays a critical role in inflammation. Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid, highly abundant in plasma, that potently regulates endothelial responses through interaction with its receptors (S1PRs). Here, we studied the role of S1PR2 ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2012-11-467191
更新日期:2013-07-18 00:00:00
abstract::Active immunization with the idiotype of follicular lymphoma induces tumor-specific immunity. T cells induced in vivo by idiotype vaccination recognize human leukocyte antigen (HLA)--restricted hypervariable but not conserved idiotype peptides. We hypothesized that idiotype-directed T-cell immunity occurs naturally an...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-02-270199
更新日期:2010-09-09 00:00:00
abstract::Selective use of recombinant human cytokines has enabled the culture of large numbers of eosinophils from human cord blood mononuclear cells, raising the possibility of their use as a model of eosinophil function. Cultured eosinophils (CE) were compared with normal-density peripheral blood eosinophils (PBE) in terms o...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract::An imbalance between cellular apoptosis and survival may be critical for the pathogenesis of lymphoma. Therefore, the gene expression pattern in lymph node preparations from patients with mantle cell lymphoma (MCL) was compared to the pattern in nonmalignant hyperplastic lymph nodes (HLs). Oligonucleotide microarray a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v98.3.787
更新日期:2001-08-01 00:00:00
abstract::It was previously shown that patients with chronic myeloid leukemia (CML) have a rare but consistently detectable population of quiescent (G0) leukemic (Philadelphia chromosome-positive and BCR-ABL-positive [BCR-ABL+]) CD34+ cells. In the study described here, most such cells expressed a primitive phenotype (CD38-, CD...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v97.3.720
更新日期:2001-02-01 00:00:00
abstract::In this issue of Blood, Larocca et al in a large prospective study show that thromboprophylaxis with low-dose aspirin is very effective in previously untreated myeloma patients with a low thromboembolic risk receiving lenalidomide with low-dose dexamethasone. ...
journal_title:Blood
pub_type: 评论,杂志文章
doi:10.1182/blood-2011-08-375089
更新日期:2012-01-26 00:00:00
abstract::Additional sex combs-like 1 (ASXL1), an epigenetic modulator, is frequently mutated in myeloid neoplasms. Recent analyses of mutant ASXL1 conditional knockin (ASXL1-MT-KI) mice suggested that ASXL1-MT alone is insufficient for myeloid transformation. In our previous study, we used retrovirus-mediated insertional mutag...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.2019004613
更新日期:2020-10-01 00:00:00
abstract::Highly enriched, phenotypically defined hematopoietic stem, Thy-1loLin-Sca-1+, and progenitor cell populations from mouse bone marrow (BM) were tested at limiting dilution for their ability to reconstitute Dexter monolayers. Several classes of BM cells can reconstitute Dexter cultures, first forming discrete "cobblest...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-08-15 00:00:00
abstract::We report the transient response of a patient with the ETV6-ABL fusion gene to imatinib mesylate (STI571). A 38-year-old man was referred with an erroneous diagnosis of Philadelphia-positive chronic myeloid leukemia in blastic transformation for treatment with the ABL tyrosine kinase inhibitor, STI571. Further investi...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.9.3465
更新日期:2002-05-01 00:00:00