Abstract:
:Antibody-mediated pure red cell aplasia is a rare but serious event resulting from the induction of neutralizing erythropoietin (Epo)-specific antibodies provoked by treatment with recombinant Epo. Because of the crucial role of CD4 T cells in humoral response, we have quantified the number of Epo-specific CD4 T cells in the blood of normal donors by in vitro stimulation. An important repertoire of preexisting Epo-specific T cells was observed in almost half of the donors, comparable with that of non-self-proteins. This observation suggests that, at the steady state, endogenous Epo weakly contributes to tolerance induction and may be ignored by the immune system. As a result, circulating Epo-specific CD4 T cells could be prone to be activated by altered batches of Epo, providing them with costimulatory signals. Our data also highlight the relevance of T-cell assays performed with normal donors to evaluate the potential immunogenicity of therapeutic proteins.
journal_name
Bloodjournal_title
Bloodauthors
Delluc S,Ravot G,Maillere Bdoi
10.1182/blood-2010-04-280875subject
Has Abstractpub_date
2010-11-25 00:00:00pages
4542-5issue
22eissn
0006-4971issn
1528-0020pii
blood-2010-04-280875journal_volume
116pub_type
杂志文章相关文献
BLOOD文献大全abstract::Approximately 40% of children with acute myeloid leukemia (AML) who respond to initial therapy subsequently relapse. Multidimensional flow cytometry employing a standardized panel of monoclonal antibodies enables the detection of small numbers of occult leukemic cells that persist during therapy using technology adapt...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2002-10-3064
更新日期:2003-05-01 00:00:00
abstract::Human natural killer (NK) cells comprise 2 main subsets, CD56(bright) and CD56(dim) cells, that differ in function, phenotype, and tissue localization. To further dissect the heterogeneity of CD56(dim) cells, we have performed transcriptome analysis and functional ex vivo characterization of human NK-cell subsets acco...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-11-253286
更新日期:2010-08-26 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) is characterized by delayed senescence and slow accumulation of monoclonal, small lymphocytes. Basic fibroblast growth factor (bFGF) is a pleiotropic cytokine that plays a role in hematopoiesis and apoptosis. Elevated bFGF levels have been detected in urine from patients with a varie...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-02-01 00:00:00
abstract::Although molecular biologic techniques can now detect minimal numbers of residual cancer cells in patients in complete clinical remission, the clinical significance of minimal residual disease has never been conclusively established. If the detection of minimal residual disease predicts which patients will relapse, th...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-06-15 00:00:00
abstract::C-abl oncogene 1, nonreceptor tyrosine kinase (ABL1) kinase inhibitors such as imatinib mesylate (imatinib) are effective in managing chronic myeloid leukemia (CML) but incapable of eliminating leukemia stem cells (LSCs), suggesting that kinase-independent pathways support LSC survival. Given that the bone marrow (BM)...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2013-07-511907
更新日期:2014-05-22 00:00:00
abstract::Chronic myelocytic leukemia (CML) is a common neoplasm of hematopoietic pluripotent stem cells. Although the evolution from chronic phase to blast crisis (BC) in CML patients is an inevitable clinical feature, little is understood about the mechanisms responsible for the transformation. We have previously performed al...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1998-11-01 00:00:00
abstract::Recent studies of retroviral-mediated gene transfer have shown that retroviral integrations themselves may trigger nonmalignant clonal expansion of hematopoietic stem cells (HSCs) in transplant recipients. These observations suggested that previous conclusions of HSC dynamics based on gamma-retroviral gene marking sho...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-03-142661
更新日期:2008-10-15 00:00:00
abstract::The underlying pathways that lead to relapse in childhood acute lymphoblastic leukemia (ALL) are unknown. To comprehensively characterize the molecular evolution of relapsed childhood B-precursor ALL, we used human 500K single-nucleotide polymorphism arrays to identify somatic copy number alterations (CNAs) in 20 diag...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-06-165027
更新日期:2008-11-15 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) lymphocytes manifest anomalous motility and cap formation. Since these processes involve cytoskeletal proteins, vimentin from intermediate filaments of normal and CLL lymphocytes was investigated using hetero- and monoclonal antisera. The antisera reacted predominantly with a 60-kD p...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1984-02-01 00:00:00
abstract::Epithin/PRSS14, a type II transmembrane serine protease, is involved in normal epithelial development and tumor progression. Here we report, as an interacting substrate of epithin, a receptor tyrosine kinase Tie2 that is well known for important roles in the vessel stability. Epithin interacts with and degrades the Ti...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-03-275289
更新日期:2011-01-27 00:00:00
abstract::The relationship between class switch recombination (CSR) and somatic hypermutation has been unclear. By using human CD27(-) naive B cells, we investigated the somatic hypermutation and producibility of immunoglobulins (Igs) that occur after CSR. Although neither adult CD27(-) nor cord blood B cells, which showed the ...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood.v99.2.567
更新日期:2002-01-15 00:00:00
abstract::Hematopoietic stem cell (HSC)-based gene therapy holds promise for the cure of many diseases. The field is now moving toward the use of lentiviral vectors (LVs) as evidenced by 4 successful clinical trials. These trials used vesicular-stomatitis-virus-G protein (VSV-G)-LVs at high doses combined with strong cytokine-c...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2014-02-558163
更新日期:2014-08-21 00:00:00
abstract::A 64-yr-old female developed acute myeloblastic leukemia following radiation and drug therapy for colon carcinoma. The patient was heterozygous for glucose-6-phosphate dehydrogenase (G-6-PD) and displayed types A and B isoenzymes in nonhematopoietic tissue. In contrast, only type B G-6-PD was observed in peripheral bl...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1978-10-01 00:00:00
abstract::Drug-induced immune thrombocytopenia (DITP) is an adverse drug effect mediated by drug-dependent antibodies. Intravenous immunoglobulin (IVIG) is frequently used to treat DITP and primary immune thrombocytopenia (ITP). Despite IVIG's proven beneficial effects in ITP, its efficacy in DITP is unclear. We have establishe...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-02-268326
更新日期:2010-09-16 00:00:00
abstract::To know the role and initiation mechanism of the interaction of glycoprotein (GP) Ib with surface-immobilized von Willebrand factor (vWF), we examined the effect of shear stress levels on platelet binding to vWF-coated plates using a cone-and-plate type viscometer capable of loading various levels of shear stress. The...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1996-11-15 00:00:00
abstract::The mechanisms of tissue necrosis and vascular damage characteristics of certain Epstein-Barr virus (EBV)-associated lymphoproliferative disorders are unknown. The CXC chemokines interferon-gamma-inducible protein-10 (IP-10) and the monokine induced by interferon-gamma (Mig) caused tissue necrosis and vascular damage ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-11-15 00:00:00
abstract::The anti-CD20 monoclonal antibody (mAb) rituximab has revolutionized the treatment of B-cell malignancies. This unprecedented success has not only substantially changed the mindset of the clinical community about the ability of mAb to improve outcomes but has catalyzed the interest in the pharmaceutical industry to de...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-07-298356
更新日期:2011-03-17 00:00:00
abstract::The congenital sideroblastic anemias (CSAs) are relatively uncommon diseases characterized by defects in mitochondrial heme synthesis, iron-sulfur (Fe-S) cluster biogenesis, or protein synthesis. Here we demonstrate that mutations in HSPA9, a mitochondrial HSP70 homolog located in the chromosome 5q deletion syndrome 5...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2015-09-659854
更新日期:2015-12-17 00:00:00
abstract::The purpose of this study was to estimate the incidence and to establish which factors were associated with an increased risk of hemorrhagic complications in an historic cohort of 117 consecutive and unselected patients with chronic idiopathic thrombocytopenic purpura (ITP). Sixty-eight patients (58%) underwent medica...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Activated protein C is a potent anticoagulant and profibrinolytic enzyme that can be derived from the vitamin-K-dependent serine protease zymogen, protein C, by the action of thrombin. Protein C antigen concentration was determined in plasmas from normals (n = 40) and from 38 patients with intravascular coagulation as...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::Class A scavenger receptor (SRA), also known as CD204, has been shown to participate in the pathogenesis of atherosclerosis and the pattern recognition of pathogen infection. However, its role in adaptive immune responses has not been well defined. In this study, we report that the lack of SRA/CD204 promotes Toll-like...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2008-11-190033
更新日期:2009-06-04 00:00:00
abstract::Little is known about the role of active immunization in suppressing undesirable immune responses. Because CD8alpha(+) dendritic cells (DCs) suppress certain immune responses, we tested the hypothesis that immunization of donors with host-derived CD8alpha(+) DCs will reduce host-specific donor T-cell responses. BALB/c...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2009-06-229708
更新日期:2010-01-21 00:00:00
abstract::Aggregation of high-affinity receptors for immunoglobulin E (Fc epsilon RI) on the surface of mast cells results in degranulation, a response that is potentiated by binding of stem cell factor (SCF) to its receptor Kit. We observed that one of the major initial signaling events associated with Fc epsilon RI-mediated a...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-08-2769
更新日期:2004-07-01 00:00:00
abstract::Hemophilia B Leyden is an X chromosome-linked bleeding disorder characterized by an altered developmental expression of blood coagulation factor IX. This form of hemophilia B has been found to be associated with a variety of single point mutations in the factor IX promoter region. We now describe a novel point mutatio...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1993-07-01 00:00:00
abstract::A study of the Bernard-Soulier syndrome in two unrelated families using different polyclonal antibodies in a sensitive immunoblot assay showed residual amounts of platelet membrane glycoprotein (GP) lb in the eight homozygotes, as well as the near-absence of GPlb beta and GPIX. The eight heterozygotes studied showed a...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1988-09-01 00:00:00
abstract::We have investigated the utility of the green fluorescent protein (GFP) to serve as a marker to assess retroviral gene transfer into hematopoietic cells and as a tool to identify and enrich for cells expressing high levels of the vector-encoded transcript. GFP, by virtue of a naturally occurring chromophore encoded in...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1997-09-01 00:00:00
abstract::As mouse models have become commonplace for studying hemostasis and thrombosis, we considered whether the mouse system had utility for assessing genetic alterations in platelet receptors. Platelets from 5 mouse strains (C57BL/6 [C57], FVB/N [FVB], BALB/c, C3H/He, and 129Sv) showed only minor differences in the express...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2003-10-3721
更新日期:2004-05-01 00:00:00
abstract::The evidence for a regulatory role of acidic isoferritins on hemopoiesis is not entirely consistent with our knowledge of ferritin biochemistry, and no clear picture of this phenomeonon has emerged. In the present study, we have been unable to confirm a consistent effect of purified heart (acidic), spleen (basic), or ...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1986-02-01 00:00:00
abstract::Umbilical cord blood (UCB) is an attractive cell source for hematopoietic cell transplantation (HCT). Here we examine whether the combination of homeobox B4 (HOXB4) and Delta-1 ligand (DL) synergize when used together. Monkey and human UCB CD34(+) cells were transduced with a HOXB4-expressing gammaretroviral vector an...
journal_title:Blood
pub_type: 杂志文章
doi:10.1182/blood-2010-05-286062
更新日期:2010-12-23 00:00:00
abstract::Kinetic studies have been carried out to investigate the functional heterogeneity previously observed in populations of human marrow or peripheral blood cells separated by velocity sedimentation. The results obtained confirm the earlier results, in that slowly-sedimenting cells were found to stimulate both colony form...
journal_title:Blood
pub_type: 杂志文章
doi:
更新日期:1975-06-01 00:00:00