Abstract：
:The supernatants of hybrids produced by fusion of splenocytes from normal, germ free, nude and neonatal BALB/c mice with mouse NS-1 myeloma cells were examined for immunofluorescence reactivity with viable and acetone fixed monolayers of syngeneic mouse fibroblasts and mouse 3T3 cells and with viable cell suspensions of syngeneic mouse erythrocytes and thymocytes. The supernatants of 70 out of 419 (16.7%) hybrids from normal mice, 87 out of 627 (13.9%) from germ free mice, 28 out of 240 (11.7%) from nude mice and 42 out of 1020 (4%) from neonatal mice reacted with 8 different intracellular structures in mouse fibroblasts and 3T3 cells, and with rat and human fibroblasts. The reactive intracellular structures comprised stress fibres, intermediate filaments, cell membrane associated, golgi complex, cytoplasm, cytoplasmic vesicles, mitochondria and nuclei. Of 45 stable clones derived by limiting dilution, 43 produced IgM antibodies and two IgG2b antibodies. Only one out of the 2,306 (0.04%) hybrids produced an autoantibody to the surface membrane of mouse thymocytes. These results show that B cells with reactivity towards different intracellular structures are present in the normal B cell repertoire whereas surface reactive B cells may be deleted or more profoundly suppressed or anergic and unable to form viable Ig producing hybridomas.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Underwood JR,Pedersen JS,Chalmers PJ,Toh BHsubject
Has Abstractpub_date
1985-05-01 00:00:00pages
417-26issue
2eissn
0009-9104issn
1365-2249journal_volume
60pub_type
杂志文章abstract：:Lymphocytotoxic activity has been found by previous investigators in multiple sclerosis (MS) sera. We confirmed the presence of this activity in MS sera using techniques designed to eliminate possible sources of erroneous conclusion not considered in previous studies. We further characterized this activity and found i...
journal_title：Clinical and experimental immunology
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更新日期：1983-07-01 00:00:00
abstract：:Recent evidence suggests that T cell apoptosis could be involved in the pathogenesis of HIV-1 infection. As the progression of HIV-2 associated disease appears to be slower than that of HIV-1, we investigated whether there were differences in the degree of T cell death and apoptosis in peripheral blood mononuclear cel...
journal_title：Clinical and experimental immunology
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doi：10.1111/j.1365-2249.1994.tb06123.x
更新日期：1994-11-01 00:00:00
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journal_title：Clinical and experimental immunology
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doi：10.1111/j.1365-2249.1992.tb06969.x
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journal_title：Clinical and experimental immunology
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更新日期：1987-08-01 00:00:00
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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更新日期：1985-07-01 00:00:00
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journal_title：Clinical and experimental immunology
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更新日期：1987-03-01 00:00:00
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journal_title：Clinical and experimental immunology
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更新日期：1985-04-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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更新日期：1976-09-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1976-11-01 00:00:00
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pub_type： 杂志文章
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journal_title：Clinical and experimental immunology
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doi：
更新日期：1977-09-01 00:00:00
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更新日期：1987-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2000-09-01 00:00:00
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
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更新日期：2018-10-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1975-02-01 00:00:00
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更新日期：2014-09-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-07-01 00:00:00
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更新日期：2002-12-01 00:00:00
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pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章,评审
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更新日期：2008-01-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1985-03-01 00:00:00
