Abstract:
:Type 1 IFN is thought to be implicated in the autoimmune process of SLE. Plasmacytoid dendric cells (DC), which are natural IFN-alpha producing cells, play a pivotal epipathogenic role in SLE. The present study was undertaken to investigate the phenotypic characteristics of peripheral blood DC in SLE patients in comparison with those of healthy controls. Samples from 20 SLE patients and 18 healthy controls were studied. Three-colour flow cytometry was performed to identify myeloid DC, as CD11c(+) lineage marker(-), and HLA-DR(+) cells and plasmacytoid DC, as BDCA-2(+) linage marker(-), and HLA-DR(+) cells. We used the whole blood 'lyse/no-wash' procedure, which allows precise counting of peripheral blood DC. BDCA-2(+) plasmacytoid DC and CD11c(+) myeloid DC were reduced in SLE patients compared with controls. Similarly, BDCA-3(+) DC were reduced in SLE patients. These results indicated that SLE patients had a reduced number of both BDCA-2(+) plasmacytoid DC and CD11c(+) myeloid DC. These alternations of the DC subset may drive the autoimmune response in SLE.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Migita K,Miyashita T,Maeda Y,Kimura H,Nakamura M,Yatsuhashi H,Ishibashi H,Eguchi Kdoi
10.1111/j.1365-2249.2005.02897.xsubject
Has Abstractpub_date
2005-10-01 00:00:00pages
84-91issue
1eissn
0009-9104issn
1365-2249pii
CEI2897journal_volume
142pub_type
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