Abstract：
:Summary The interaction between stroma and blood cells in the human spleen has received little attention, despite their well-defined roles during blood cell development in bone marrow. We have reported previously that human spleen-derived fibroblasts display a differentiated myofibroblast phenotype and constitutively express a biologically active form of membrane interleukin (IL)-15 that can drive co-cultured CD34(+) blood cells to differentiate into activated natural killer (NK) cells. Here, we show that, in addition to NK cells, CD34/fibroblast co-cultures also yield myeloid CD1a(+)CD38(+)CD68(+)CD86(+) HLA-DR(+)CD14(-)CD80(-) dendritic cells (DCs) after 3-4 weeks in culture. We found that DC development depended on endogenously secreted stromal macrophage colony-stimulating factor (M-CSF) and CD40/CD40L interaction rather than on fibroblast- and CD34-derived membrane IL-15. CD1a(+) cells were necessary for co-produced NK cells to acquire lytic functions by a mechanism involving cell-to-cell contact and DC-derived IL-12. This study highlights the importance of spleen myofibroblasts in the in vitro generation of two distinct cell types (DC and NK cells) from the innate immune system and suggests that the human spleen is involved in the generation of NK cells from circulating progenitors.
journal_name
Clin Exp Immunoljournal_title
Clinical and experimental immunologyauthors
Briard D,Azzarone B,Brouty-Boyé Ddoi
10.1111/j.1365-2249.2005.02792.xsubject
Has Abstractpub_date
2005-05-01 00:00:00pages
265-73issue
2eissn
0009-9104issn
1365-2249pii
CEI2792journal_volume
140pub_type
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journal_title：Clinical and experimental immunology
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doi：10.1111/j.1365-2249.1995.tb05573.x
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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journal_title：Clinical and experimental immunology
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doi：
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journal_title：Clinical and experimental immunology
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更新日期：1982-02-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1995.tb02273.x
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1981-05-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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更新日期：2010-01-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1977-12-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.1997.tb08325.x
更新日期：1997-11-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/j.1365-2249.2011.04399.x
更新日期：2011-07-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1980-07-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：10.1111/cei.12598
更新日期：2015-06-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1978-07-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-12-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1987-03-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-09-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1983-12-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-06-01 00:00:00
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pub_type： 杂志文章
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pub_type： 杂志文章
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更新日期：2013-10-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1986-11-01 00:00:00
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pub_type： 杂志文章
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更新日期：1999-12-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
doi：
更新日期：1984-10-01 00:00:00
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pub_type： 杂志文章,评审
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更新日期：2018-05-01 00:00:00
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journal_title：Clinical and experimental immunology
pub_type： 杂志文章
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