Chronic ethanol intake-induced changes in open-field behavior and calcium/calmodulin-dependent protein kinase IV expression in nucleus accumbens of rats: naloxone reversal.

abstract：AIM:Targeting the VEGF/VEGF receptor (VEGFR) pathway has proved to be an effective antiangiogenic approach for cancer treatment. Here, we identified 6-((2-((3-acetamidophenyl)amino)pyrimidin-4-yl)oxy)-N-phenyl-1-naphthamide (designated herein as DW10075) as a novel and highly selective inhibitor of VEGFRs. METHODS:In ...

journal_title：Acta pharmacologica Sinica

authors： Li MY,Lv YC,Tong LJ,Peng T,Qu R,Zhang T,Sun YM,Chen Y,Wei LX,Geng MY,Duan WH,Xie H,Ding J

更新日期：2016-03-01 00:00:00

abstract：AIM:To investigate modulation of antagonist and agonist binding to adenosine A1 receptors by MgCl2 and 5 -guanylimidodiphosphate (Gpp(NH)p) using rat brain membranes and the A1 antagonist [3H]-8-cyclopentyl-1,3-dipropylxanthine ([3H]DPCPX) and the A1 agonist [3H]-2-chloro-N6-cyclopentyladenosine ([3H]CCPA). METHODS:Pa...

journal_title：Acta pharmacologica Sinica

authors： Finlayson K,Maemoto T,Butcher SP,Sharkey J,Olverman HJ

更新日期：2003-08-01 00:00:00

abstract：AIM:To determine the role of dietary low histamine on the seizure development of pentylenetetrazol (PTZ)-induced kindling in rats. METHODS:After 14 d of feeding on a low histamine diet (LH, containing 0.145 mumol/g of histamine), the rats were chemically kindled by repeated intraperitoneal injection of a subconvulsant...

journal_title：Acta pharmacologica Sinica

authors： Jin CL,Sakurai E,Kiso Y,Luo JH,Yanai K,Chen Z

更新日期：2005-04-01 00:00:00

abstract：AIM:To investigate the pharmacokinetic profile and tissue distribution of a novel phosphodiesterase type 5 inhibitor, 5-ethyl-2-{5-[4-(2-hydroxy-ethyl)-piperazine-1-sulfonyl]-2-propoxy-phenyl}-7-propyl-3,5-dihydro-pyrrolo(3,2-d)pyrimidin-4-one (SK-3530), in rats after administration of the (14)C-labeled compound. METH...

journal_title：Acta pharmacologica Sinica

authors： Yoo HH,Kim NS,Im GJ,Kim DH

更新日期：2007-08-01 00:00:00

abstract：:Induced pluripotent stem cells (iPSCs) have become an essential research platform to study different human diseases once being discovered by Dr. Shinya Yamanaka in 2006. Another breakthrough in biomedical research is the application of CRISPR/Cas9 system for genome editing in mammalian cells. Although numerous studies...

journal_title：Acta pharmacologica Sinica

authors： Geng BC,Choi KH,Wang SZ,Chen P,Pan XD,Dong NG,Ko JK,Zhu H

更新日期：2020-11-01 00:00:00

abstract：AIM:To develop a robust, cell-based, high-content screening (HCS) assay based on receptor internalization for the identification of novel modulators of the epidermal growth factor receptor (EGFR). METHODS:Agonist-induced receptor internalization is part of the signaling cascade of EGFR. Fluorescent-tagged epidermal gr...

journal_title：Acta pharmacologica Sinica

authors： Wang J,Xie X

更新日期：2007-10-01 00:00:00

abstract：AIM:FTY720 is a new synthetic immunosuppressive agent which has a unique mechanism of action and induces long-term graft acceptance in rat and dog allotransplantation as prophylactic administration. The present study investigated whether FTY720 was able to rescue ongoing acute rejection of solid organ transplants in a ...

journal_title：Acta pharmacologica Sinica

authors： Wang MH,Milekhin V,Zhang H,Huang HZ

更新日期：2003-09-01 00:00:00

abstract：AIM:To investigate the modulatory effect of CJZ3, a lomerizine derivative, on P-glycoprotein (P-gp) function in rat brain microvessel endothelial cells (RBMEC). METHODS:RBMEC were isolated and cultured in Dulbecco modified Eagle medium/F12 (1:1) medium, and the amount of intracellular rhodamine 123 (Rh123) was determi...

journal_title：Acta pharmacologica Sinica

authors： Ji BS,He L,Li XQ,Liu GQ

更新日期：2006-04-01 00:00:00

abstract：AIM:To evaluate the effect of C-C chemokine receptor 3 (CCR3) blockade on pulmonary inflammation and mucus production in allergic mice. METHODS:We used the synthetic peptide of the CCR3 NH2-terminal as the immunizing antigen and generated murine monoclonal antibody against the human CCR3. In addition, the generated an...

journal_title：Acta pharmacologica Sinica

authors： Wang K,Shen HH,Li W,Huang HQ

更新日期：2007-11-01 00:00:00

abstract：AIM:To examine whether the cell growth inhibitory effect of the combination of baicalin and baicalein is related to apoptosis. Moreover, to determine whether the expression of some apoptosis-related proteins is regulated by the ERK/p38 MAPK pathway. METHODS:Cell viability was measured using a 3-(4,5-dimethylthiazol-2-...

journal_title：Acta pharmacologica Sinica

authors： Zhou QM,Wang S,Zhang H,Lu YY,Wang XF,Motoo Y,Su SB

更新日期：2009-12-01 00:00:00

abstract：AIM:To examine the role of cholinergic neurons in the nucleus raphe magnus (NRM) in noxious heat stimulation and in the effects of morphine-induced antinociception by rats. METHODS:After the cholinergic neuron selective toxin, AF64A, was microinjected into the NRM, we examined changes in the antinociceptive threshold ...

journal_title：Acta pharmacologica Sinica

authors： Abe K,Ishida K,Kato M,Shigenaga T,Taguchi K,Miyatake T

更新日期：2002-11-01 00:00:00

abstract：AIM:To examine the in vitro antitumor activity of resveratrol against multiple myeloma (MM) cell lines (RPMI 8226, U266, and KM3), and the mechanisms involved. METHODS:The growth inhibition of resveratrol was determined by 3-(4, 5-dimethyl-2-thiazyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay. The effect of resve...

journal_title：Acta pharmacologica Sinica

authors： Sun CY,Hu Y,Guo T,Wang HF,Zhang XP,He WJ,Tan H

更新日期：2006-11-01 00:00:00

abstract：:Diabetic retinopathy (DR) is a common complication of diabetes and has been recognized as the leading cause of blindness in adults. Several interrelated molecular pathways are involved in the development of DR. Microvesicles (MVs) are cell membrane vesicles, which carry many biologic molecules, such as mRNAs, microRNA...

journal_title：Acta pharmacologica Sinica

authors： Zhang W,Chen S,Liu ML

更新日期：2018-01-01 00:00:00

abstract：AIM:Pirarubicin (THP) is recently found to be effective in treating patients with advanced, relapsed or recurrent high-grade osteosarcoma. In this study, the effects of THP on the multidrug-resistant (MDR) osteosarcoma cells were assessed, and the underlying mechanisms for the disruption of cell cycle kinetics by THP w...

journal_title：Acta pharmacologica Sinica

authors： Zheng SE,Xiong S,Lin F,Qiao GL,Feng T,Shen Z,Min DL,Zhang CL,Yao Y

更新日期：2012-06-01 00:00:00

abstract：AIM:Pim-2 is a short-lived serine/threonine kinase, which plays a key role in metastasis of breast cancer through persistent activation of STAT3. Although the crystal structure of Pim-2 has been reported, but thus far no specific Pim-2-targeted compounds have been reported. In this study, we identified a novel Pim-2 in...

journal_title：Acta pharmacologica Sinica

authors： Zhao YQ,Yin YQ,Liu J,Wang GH,Huang J,Zhu LJ,Wang JH

更新日期：2016-09-01 00:00:00

abstract：AIM:To investigate the effect of melittin (Mel) on papillary muscles of guinea pigs. METHODS:Contraction of papillary muscles were examined by conventional method and action potentials (AP) were recorded by standard glass microelectrode technique. RESULTS:Mel (0.5, 3 micromol/L) significantly increased the contractil...

journal_title：Acta pharmacologica Sinica

authors： Zhang YL,Yang S,Shi NC,Jiang MH

更新日期：2001-08-01 00:00:00

abstract：AIM:In order to elucidate the molecular mechanism underlying the cardioprotection afforded by schisandrin B (Sch B), the effect of Sch B treatment on the sensitivity of mitochondria to Ca2+-stimulated permeability transition (PT) was investigated in rat hearts under normal and ischemia-reperfusion (I-R) conditions. RE...

journal_title：Acta pharmacologica Sinica

authors： Chiu PY,Leung HY,Siu AH,Poon MK,Ko KM

更新日期：2007-10-01 00:00:00

abstract：:Astragaloside IV (AsIV) is an active saponin extracted from Astragalus membranaceus, which has shown cardioprotective effects in a number of experimental animals. In this study we investigated the molecular mechanisms by which AsIV attenuated the myocardial ischemia reperfusion (MI/R)-induced injury in vitro and in vi...

journal_title：Acta pharmacologica Sinica

authors： Yin B,Hou XW,Lu ML

更新日期：2019-05-01 00:00:00

abstract：AIM:To evaluate retrospectively the association of cytochrome P450 3A (CYP3A) and ATP-binding cassette sub-family B member 1 (ABCB1) gene polymorphisms with the pharmacokinetics of cyclosporine A (CsA) in Chinese renal transplant patients. METHODS:One hundred and twenty-six renal transplant patients were recruited. Bl...

journal_title：Acta pharmacologica Sinica

authors： Meng XG,Guo CX,Feng GQ,Zhao YC,Zhou BT,Han JL,Chen X,Shi Y,Shi HY,Yin JY,Peng XD,Pei Q,Zhang W,Wang G,He M,Liu M,Yang JK,Zhou HH

更新日期：2012-12-01 00:00:00

abstract：AIM:To investigate whether autologous transplantation of adult stem cells could improve post-infarcted heart function. METHODS:Bone marrow mononuclear cells (MNCs) were isolated from adult rabbits' tibias after coronary ligation. These cells were exposed to 5-azacytidine 10 micromol/L for 24 h on the third day of cult...

journal_title：Acta pharmacologica Sinica

authors： Lin GS,Lü JJ,Jiang XJ,Li XY,Li GS

更新日期：2004-07-01 00:00:00

abstract：AIM:To investigate the effects of resveratrol on interleukin-1beta (IL-1beta) induced catabolism in bone marrow mesenchymal stem cell (MSC) derived chondrocytes cultured on chitosan-gelatin scaffolds (CGS). METHODS:The chondrogenesis of alginate-encapsulated MSCs was evaluated by toluidine blue staining, RT-PCR, and i...

journal_title：Acta pharmacologica Sinica

authors： Lei M,Liu SQ,Liu YL

更新日期：2008-11-01 00:00:00

abstract：AIM:To investigate molecular mechanism of testis development and spermatogenesis. METHODS:A human testis cDNA microarray was hybridized with probes from human adult testis, embryo testis and human sperm, and the differential expressed clones were sequenced and analyzed. Expression of PIAS-NY gene was analyzed by RT-PC...

journal_title：Acta pharmacologica Sinica

authors： Zheng Y,Zhou ZM,Yin LL,Li JM,Sha JH

更新日期：2004-08-01 00:00:00

abstract：AIM:To develop a simple method for monitoring protein localization of epidermal growth factor (EGF) in living cells. METHODS:Enhanced green fluorescent protein (EGFP) was used as an autofluorescent tag to label EGF ligands. SDS-PAGE and Western blot analysis were used to detect the expression of the EGFP-tagged EGF (E...

journal_title：Acta pharmacologica Sinica

authors： Jiang H,Zhang J,Shi BZ,Xu YH,Li ZH,Gu JR

更新日期：2007-01-01 00:00:00

abstract：AIM:To investigate the antidepressant-like effects of a novel 5-HT3 receptor antagonist N-(benzo[d]thiazol-2-yl)-3-methoxyquinoxalin-2-carboxamide (6z) in acute and chronic murine models of depression. METHODS:5-HT3 receptor antagonism was examined in guinea pig ileum in vitro. A tail suspension test (TST) was used as...

journal_title：Acta pharmacologica Sinica

authors： Gupta D,Radhakrishnan M,Kurhe Y,Thangaraj D,Prabhakar V,Kanade P

更新日期：2014-12-01 00:00:00

abstract：AIM:To determine whether basophils expressed human leucocyte antigen (HLA) class II molecules. METHODS:Basophils in umbilical cord blood were separated and purified with methods of density gradient centrifugation and immunomagnetic microbeads. The isolated basophils were cultured in RPMI-1640 plus 10 % fetal calf seru...

journal_title：Acta pharmacologica Sinica

authors： Xie ML,Wu YG

更新日期：2002-07-01 00:00:00

abstract：AIM:To search for novel effective P-glycoprotein (P-gp) reversal agents in the blood-brain barrier (BBB). METHODS:Using rhodamine123 (Rh123) to examine the functional activity of P-gp in cultured bovine brain capillary endothelial cells (BCEC) and screen various principles on P-gp modulation in BBB. RESULTS:All of te...

journal_title：Acta pharmacologica Sinica

authors： He L,Liu GQ

更新日期：2002-07-01 00:00:00

abstract：:Emerging evidence demonstrates that a c-Met antibody-drug conjugate (ADC) has superior efficacy and safety profiles compared with those of currently available small molecules or antibody inhibitors for the treatment of c-Met-overexpressing cancers. Here we described both the in vitro and in vivo efficacies of SHR-A140...

journal_title：Acta pharmacologica Sinica

authors： Yang CY,Wang L,Sun X,Tang M,Quan HT,Zhang LS,Lou LG,Gou SH

更新日期：2019-07-01 00:00:00

abstract：:Cysteine-rich peptides from the venom of cone snails (Conus) target a wide variety of different ion channels. One family of conopeptides, the alpha-conotoxins, specifically target different isoforms of nicotinic acetylcholine receptors (nAChRs) found both in the neuromuscular junction and central nervous system. This ...

journal_title：Acta pharmacologica Sinica

authors： Azam L,McIntosh JM

更新日期：2009-06-01 00:00:00

abstract：AIM:To characterize the in vitro bioactivities of rhodanine derivatives as novel peroxisome proliferator-activated receptor (PPAR) gamma modulators, based on a hit (SH00012671) identified during high-throughput screening (HTS) of a diverse synthetic compound library, and to preliminarily elucidate the structure-activit...

journal_title：Acta pharmacologica Sinica

authors： Liu Q,Zhang YY,Lu HL,Li QY,Zhou CH,Wang MW

更新日期：2007-12-01 00:00:00

abstract：:Activation of the Ca2+/calmodulin-dependent protein kinase II isoform δA (CaMKIIδA) disturbs intracellular Ca2+ homeostasis in cardiomyocytes during chronic heart failure (CHF). We hypothesized that upregulation of CaMKIIδA in cardiomyocytes might enhance Ca2+ leak from the sarcoplasmic reticulum (SR) via activation o...

journal_title：Acta pharmacologica Sinica

authors： Gui L,Guo X,Zhang Z,Xu H,Ji YW,Wang RJ,Zhu JH,Chen QH

更新日期：2018-10-01 00:00:00