Abstract:
AIM:To determine whether basophils expressed human leucocyte antigen (HLA) class II molecules. METHODS:Basophils in umbilical cord blood were separated and purified with methods of density gradient centrifugation and immunomagnetic microbeads. The isolated basophils were cultured in RPMI-1640 plus 10 % fetal calf serum at 37 in a humidified atmosphere with 5 % CO2 and stimulated with granulocyte-macrophage colony-stimulating factor (GM-CSF) or interferon (IFN)-gamma for 20-60 h. The expression of HLA class II molecules on basophils was measured with fluorescence activated cell sorter (FACS). RESULTS:The purity of isolated basophils was > or = 83.5 %. After treated with GM-CSF 10 microg/L or IFN-gamma 100 kU/L, the expression of HLA class II molecules became detectable on membranous surface and at a higher level at 20 h, the percentage of expression was 10.2 %+/-2.1 % and 11.3 %+/-1.0 %, respectively. CONCLUSION:Basophils possessed the potency of HLA class II molecular expression.
journal_name
Acta Pharmacol Sinjournal_title
Acta pharmacologica Sinicaauthors
Xie ML,Wu YGsubject
Has Abstractpub_date
2002-07-01 00:00:00pages
645-8issue
7eissn
1671-4083issn
1745-7254journal_volume
23pub_type
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