Short hairpin RNA-mediated inhibition of HSV-1 gene expression and function during HSV-1 infection in Vero cells.

Abstract:

AIM:To evaluate the efficiency of 3 short hairpin RNA (shRNA) interfering with the herpes simplex virus type 1 (HSV-1) gene coding glycoprotein D (gD) for inhibiting the gD expression and virus replication in vitro. METHODS:Vero cells were selected for an in vitro model of infection. Three shRNA sequences (shRNAgD1, -gD2, and -gD3) targeting specifically the gD gene of HSV-1 were selected for evaluating the antiviral effects. The antiviral effects of shRNA in the cells infected with HSV-1 were evaluated by cytopathic effect (CPE) observations and plaque assays. The transcription level of viral RNA and the gD expression were studied by RT-PCR, Western blotting, and flow cytometry. RESULTS:With the 3 shRNA at a final concentration of 120 nmol/L, a significant inhibition of CPE in the HSV-1-infected cells was observed. The ED50 of shRNA-gD1, gD2, and gD3 were 48.74+/-2.57, 57.13+/-3.24, and 114.64+/-5.12 nmol/L, respectively. The gD gene decreased significantly after viral infection in the Vero cells pretreated with shRNA compared to the virus group. The expressions of the gD protein, determined by Western blotting and flow cytometry, were also drastically decreased in shRNA-transfected cells. CONCLUSION:Exogenous shRNA molecules can suppress the HSV-1 gD expression. They are inhibitors of HSV replication during infection in Vero cells.

journal_name

Acta Pharmacol Sin

authors

Liu YY,Deng HY,Yang G,Jiang WL,Grossin L,Yang ZQ

doi

10.1111/j.1745-7254.2008.00828.x

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

975-82

issue

8

eissn

1671-4083

issn

1745-7254

journal_volume

29

pub_type

杂志文章
  • Population pharmacokinetics of clozapine and its primary metabolite norclozapine in Chinese patients with schizophrenia.

    abstract:AIM:To develop a combined population pharmacokinetic model (PPK) to assess the magnitude and variability of exposure to both clozapine and its primary metabolite norclozapine in Chinese patients with refractory schizophrenia via sparse sampling with a focus on the effects of covariates on the pharmacokinetic parameters...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2012.71

    authors: Li LJ,Shang DW,Li WB,Guo W,Wang XP,Ren YP,Li AN,Fu PX,Ji SM,Lu W,Wang CY

    更新日期:2012-11-01 00:00:00

  • HIV entry inhibitors: a new generation of antiretroviral drugs.

    abstract::AIDS is presently treatable, and patients can have a good prognosis due to the success of highly active antiretroviral therapy (HAART), but it is still not curable or preventable. High toxicity of HAART, and the emergence of drug resistance add to the imperative to continue research into new strategies and interventio...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章,评审

    doi:10.1111/j.1745-7254.2005.00193.x

    authors: Krambovitis E,Porichis F,Spandidos DA

    更新日期:2005-10-01 00:00:00

  • DW10075, a novel selective and small-molecule inhibitor of VEGFR, exhibits antitumor activities both in vitro and in vivo.

    abstract:AIM:Targeting the VEGF/VEGF receptor (VEGFR) pathway has proved to be an effective antiangiogenic approach for cancer treatment. Here, we identified 6-((2-((3-acetamidophenyl)amino)pyrimidin-4-yl)oxy)-N-phenyl-1-naphthamide (designated herein as DW10075) as a novel and highly selective inhibitor of VEGFRs. METHODS:In ...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2015.117

    authors: Li MY,Lv YC,Tong LJ,Peng T,Qu R,Zhang T,Sun YM,Chen Y,Wei LX,Geng MY,Duan WH,Xie H,Ding J

    更新日期:2016-03-01 00:00:00

  • Development of a quantitative, cell-based, high-content screening assay for epidermal growth factor receptor modulators.

    abstract:AIM:To develop a robust, cell-based, high-content screening (HCS) assay based on receptor internalization for the identification of novel modulators of the epidermal growth factor receptor (EGFR). METHODS:Agonist-induced receptor internalization is part of the signaling cascade of EGFR. Fluorescent-tagged epidermal gr...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1111/j.1745-7254.2007.00640.x

    authors: Wang J,Xie X

    更新日期:2007-10-01 00:00:00

  • Hispidulin mediates apoptosis in human renal cell carcinoma by inducing ceramide accumulation.

    abstract::Hispidulin, a polyphenolic flavonoid extracted from the traditional Chinese medicinal plant S involucrata, exhibits anti-tumor effects in a wide array of human cancer cells, mainly through growth inhibition, apoptosis induction and cell cycle arrest. However, its precise anticancer mechanisms remain unclear. In this s...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2017.154

    authors: Gao H,Gao MQ,Peng JJ,Han M,Liu KL,Han YT

    更新日期:2017-12-01 00:00:00

  • Localized Ca2+ uncaging induces Ca2+ release through IP3R in smooth muscle.

    abstract:AIM:Our previous study indicated that there are two types of Ca2+ release events seen in intact mouse bladder tissue. In this study our aim is to investigate the mechanism that underlies the phenomena of Ca2+ release in smooth muscle. METHODS:Single cells were isolated and tissue segments were prepared by cutting the ...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1111/j.1745-7254.2006.00389.x

    authors: Wang M,Chen Z,Xing Y,Zhang X,Dong XZ,Ji GJ

    更新日期:2006-07-01 00:00:00

  • Association between fibroblast growth factor 7 and the risk of chronic obstructive pulmonary disease.

    abstract:AIM:Fibroblast growth factor 7 (FGF7) is involved in a number of physiological and pathological processes, including lung disease. However, relatively little is known about the effect of FGF7 gene polymorphisms on chronic obstructive pulmonary disease (COPD) susceptibility. This study aimed to investigate the associati...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2012.69

    authors: Xu SC,Kuang JY,Liu J,Ma CL,Feng YL,Su ZG

    更新日期:2012-08-01 00:00:00

  • Alteration of airway responsiveness mediated by receptors in ovalbumin-induced asthmatic E3 rats.

    abstract:AIM:Airway hyperresponsiveness is a constant feature of asthma. The aim of the present study was to investigate airway hyperreactivity mediated by contractile and dilative receptors in an ovalbumin (OVA)-induced model of rat asthma. METHODS:Asthmatic E3 rats were prepared by intraperitoneal injection with OVA/aluminum...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2009.61

    authors: Long JW,Yang XD,Cao L,Lu SM,Cao YX

    更新日期:2009-07-01 00:00:00

  • Enhancement of binding activity of soluble human CD40 to CD40 ligand through incorporation of an isoleucine zipper motif.

    abstract:AIM:To investigate the effect of incorporation of an isoleucine zipper (IZ) motif into CD40 on binding activity of CD40 for the CD40 ligand (CD40L). METHODS:Prokaryotic expression vectors for 2 soluble CD40 derivatives, shCD40His and shCD40IZ containing an IZ domain, were constructed and expressed in Escherichia coli....

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1111/j.1745-7254.2006.00285.x

    authors: He XH,Xu LH,Liu Y

    更新日期:2006-03-01 00:00:00

  • Comparisons of somatic action potentials from dispersed and intact rat nodose ganglia using patch-clamp technique.

    abstract:AIM:To differentiate the electrophysiological characteristics of somatic action potentials (AP) from isolated Neo and Juv nodose sensory neurons (NSN) and those from slices of intact Juv and adult rat nodose ganglia. METHODS:For isolated cell recordings nodose ganglia from 3-8 d old Neo and 4 weeks old Juv rats were d...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:

    authors: Li BY,Schild JH

    更新日期:2002-06-01 00:00:00

  • Yeast two-hybrid screening for proteins that interact with alpha1-adrenergic receptors.

    abstract:AIM:To find novel proteins that may bind to alpha1A-adrenergic receptor (alpha1A-AR) and investigate their interactions with the other two alpha1-AR subtypes (alpha1B-AR and alpha1D-AR) with an expectation to provide new leads for the function study of the receptors. METHODS:Yeast two-hybrid assay was performed to scr...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:

    authors: Zhang T,Xu Q,Chen FR,Han QD,Zhang YY

    更新日期:2004-11-01 00:00:00

  • Isolation of acetylcholinesterase G4 and G1 molecular isoforms from rat cortex.

    abstract:AIM:To isolate the G4 and G1 molecular forms of acetylcholinesterase in rat cortex for interpreting the therapeutical effect of the AChE inhibitor drugs. METHODS:Size exclusion chromatography and ultracentrifugation were used to isolate and ascertain the G4 and G1 molecular forms of AChE. RESULTS:After size exclusion...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:

    authors: Zhao Q,Tang XC

    更新日期:2002-02-01 00:00:00

  • In vitro metabolic characteristics of cytochrome P-450 2A6 in Chinese liver microsomes.

    abstract:AIM:To investigate the metabolic characteristics of cytochrome P-450 C YP2A6 in human liver microsomes. METHODS:Cytochrome P-450 enzyme activities were measured by biochemical assays. Xenobiotics were employed to observe their effects on CYP2A6 in vitro. The kinetics of coumarin 7-hydroxylase was determined, and the c...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:

    authors: Xia XY,Peng RX,Yu JP,Wang H,Wang J

    更新日期:2002-05-01 00:00:00

  • Isomer-selective distribution of 3-n-butylphthalide (NBP) hydroxylated metabolites, 3-hydroxy-NBP and 10-hydroxy-NBP, across the rat blood-brain barrier.

    abstract:AIM:To investigate the mechanisms underlying the isomer-selective distribution of 3-n-butylphthalide (NBP) hydroxylated metabolites, 3-hydroxy-NBP (3-OH-NBP) and 10-hydroxy-NBP (10-OH-NBP), across the blood brain barrier (BBB). METHODS:After oral administration of NBP (20 mg/kg) to rats, the pharmacokinetics of two ma...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2015.64

    authors: Diao XX,Zhong K,Li XL,Zhong DF,Chen XY

    更新日期:2015-12-01 00:00:00

  • Allosteric activation mechanism of the cys-loop receptors.

    abstract::Binding of a neurotransmitter to its ionotropic receptor opens a distantly located ion channel, a process termed allosteric activation. Here we review recent advances in the molecular mechanism by which the cys-loop receptors are activated with emphasis on the best studied nicotinic acetylcholine receptors (nAChRs). W...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章,评审

    doi:10.1038/aps.2009.51

    authors: Chang YC,Wu W,Zhang JL,Huang Y

    更新日期:2009-06-01 00:00:00

  • Effect of unsaturated fatty acid on muscarinic current in guinea pig gastric antral circular myocytes.

    abstract:AIM:To investigate the effect of ectogenesis unsaturated fatty acid on carbachol-induced muscarinic current (ICCh) and its mechanism. METHODS:Using the whole-cell patch-clamp technique, ICCh was recorded in single smooth muscle cell isolated from the antral circular smooth muscles of guinea-pig stomach. RESULTS:Arach...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:

    authors: Cui YF,Jin H,Guo HS,Li L,Yu YC,Xu WX

    更新日期:2003-03-01 00:00:00

  • M2 muscarinic receptor of spinal cord mediated increase of nNOS expression in locus coeruleus during morphine withdrawal.

    abstract:AIM:To investigate the effects of different muscarinic receptor (M) subtypes in the spinal cord on the scores of naloxone-precipitated morphine-withdrawal symptoms and the changes of nNOS expression in locus coeruleus (LC). METHODS:nNOS immunohistochemistry, intrathecal injection (it), and antisense oligonucleotides (...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:

    authors: Chou WB,Zeng YM,Duan SM,Zhou WH,Gu J,Yang GD

    更新日期:2002-08-01 00:00:00

  • Sirt1 is involved in decreased bone formation in aged apolipoprotein E-deficient mice.

    abstract:AIM:Apolipoprotein E (ApoE) plays an important role in the transport and metabolism of lipids. Recent studies show that bone mass is increased in young apoE(-/-) mice. In this study we investigated the bone phenotype and metabolism in aged apoE(-/-) mice. METHODS:Femurs and tibias were collected from 18- and 72-week-o...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2015.95

    authors: Hong W,Xu XY,Qiu ZH,Gao JJ,Wei ZY,Zhen L,Zhang XL,Ye ZB

    更新日期:2015-12-01 00:00:00

  • Huperzine A ameliorates obesity-related cognitive performance impairments involving neuronal insulin signaling pathway in mice.

    abstract::Type 2 diabetes (T2D) and Alzheimer's disease (AD) share several common pathophysiological features. Huperzine A (Hup A), a Lycopodium alkaloid extracted from the Chinese herb moss Huperzia serrata, is a specific and reversible inhibitor of acetylcholinesterase, which is clinically used for the treatment of AD. In thi...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/s41401-019-0257-1

    authors: Wang HY,Wu M,Diao JL,Li JB,Sun YX,Xiao XQ

    更新日期:2020-02-01 00:00:00

  • Proteomics reveals potential non-neuronal cholinergic receptor-effectors in endothelial cells.

    abstract:AIM:The non-neuronal acetylcholine system (NNAS) in endothelial cells participates in modulating endothelial function, vascular tone, angiogenesis and inflammation, thus plays a critical role in cardiovascular diseases. In this study, we used a proteomic approach to study potential downstream receptor-effectors of NNAS...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2014.38

    authors: Zhang YY,Shen W,Zhang LC,Pan ZY,Long CL,Cui WY,Zhang YF,Wang H

    更新日期:2014-09-01 00:00:00

  • Icariside II attenuates cerebral ischemia/reperfusion-induced blood-brain barrier dysfunction in rats via regulating the balance of MMP9/TIMP1.

    abstract::Cerebral ischemia/reperfusion (I/R) results in harmful consequences during ischemic stroke, especially the disruption of the blood-brain barrier (BBB), which leads to severe hemorrhagic transformation through aggravation of edema and brain hemorrhage. Our previous study demonstrated that icariside II (ICS II), which i...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/s41401-020-0409-3

    authors: Liu MB,Wang W,Gao JM,Li F,Shi JS,Gong QH

    更新日期:2020-12-01 00:00:00

  • Action of aluminum on high voltage-dependent calcium current and its modulation by ginkgolide B.

    abstract:AIM:To investigate the effect of aluminum (Al) on high voltage-dependent calcium current (I(HVA)) and its modulation by ginkgolide B (Gin B). METHODS:The whole-cell, patch-clamp technique was used to record IHVA from acutely isolated hippocampal CA1 pyramydal neurons in rats. RESULTS:Al 0.1 mmol/L (low concentration)...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1111/j.1745-7254.2005.00073.x

    authors: Chen L,Liu CJ,Tang M,Li A,Hu XW,Du YM,Shen JJ,Lu YL,Heschler J

    更新日期:2005-05-01 00:00:00

  • Probucol suppresses human glioma cell proliferation in vitro via ROS production and LKB1-AMPK activation.

    abstract:AIM:Probucol, an anti-hyperlipidemic drug, has been reported to exert antitumor activities at various stages of tumor initiation, promotion and progression. In this study we examined whether the drug affected glioma cell growth in vitro and the underlying mechanisms. METHODS:Human glioma U87 and glioblastoma SF295 cel...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章,收录出版

    doi:10.1038/aps.2014.88

    authors: Jiang YS,Lei JA,Feng F,Liang QM,Wang FR

    更新日期:2014-12-01 00:00:00

  • Double-mismatched siRNAs enhance selective gene silencing of a mutant ALS-causing allele.

    abstract:AIM:Our previous study demonstrated an siRNA-mediated, allele-specific silencing of mutant genes that cause amyotrophic lateral sclerosis. To improve siRNA design for better therapeutic use of RNA interference, we systematically tested the base-pairing mismatch strategy in the design of asymmetric siRNA. METHODS:A nat...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1111/j.1745-7254.2008.00740.x

    authors: Geng CM,Ding HL

    更新日期:2008-02-01 00:00:00

  • Discovery of potent beta-secretase (bace-1) inhibitors by the synthesis of isophthalamide-containing hybrids.

    abstract:AIM:The aim of this study was to design and synthesize a series of high activity compounds against aspartyl protease beta-secretase (BACE-1) bearing hydroxyethylene (HE) framework. METHODS:First, we designed the small library based on our previous work and rational analysis. Subsequently, thirteen compounds were selec...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2008.26

    authors: Zhu YP,Xiao K,Yu HP,Ma LP,Xiong B,Zhang HY,Wang X,Li JY,Li J,Shen JK

    更新日期:2009-02-01 00:00:00

  • Effects of CpG oligodeoxynucleotide on transcription factors GATA-3 and T-bet mRNA expression in asthmatic mice.

    abstract:AIM:To investigate effects of CpG oligodeoxynucleotide (CpG ODN) on the mRNA expression of transcription factors GATA binding protein 3 (GATA-3) and T-box expressed in T cells (T-bet) in asthmatic mice. METHODS:An asthmatic mouse model was established and treated with CpG ODN. Total inflammatory cells and eosinophils ...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1111/j.1745-7254.2005.00157.x

    authors: Wu ZQ,Xu YP,Xiang H,Shen HH

    更新日期:2005-09-01 00:00:00

  • Regulation of the nongenomic actions of retinoid X receptor-α by targeting the coregulator-binding sites.

    abstract::Retinoid X receptor-α (RXRα), a unique member of the nuclear receptor superfamily, represents an intriguing and unusual target for pharmacologic interventions and therapeutic applications in cancer, metabolic disorders and neurodegenerative diseases. Despite the fact that the RXR-based drug Targretin (bexarotene) is c...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章,评审

    doi:10.1038/aps.2014.109

    authors: Zhang XK,Su Y,Chen L,Chen F,Liu J,Zhou H

    更新日期:2015-01-01 00:00:00

  • Autophagy in ageing and ageing-associated diseases.

    abstract::Autophagy is a cell self-digestion process via lysosomes that clears "cellular waste", including aberrantly modified proteins or protein aggregates and damaged organelles. Therefore, autophagy is considered a protein and organelle quality control mechanism that maintains normal cellular homeostasis. Dysfunctional auto...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章,评审

    doi:10.1038/aps.2012.188

    authors: He LQ,Lu JH,Yue ZY

    更新日期:2013-05-01 00:00:00

  • Substrate-dependent modulation of the catalytic activity of CYP3A by erlotinib.

    abstract:AIM:To ascertain the effects of erlotinib on CYP3A, to investigate the amplitude and kinetics of erlotinib-mediated inhibition of seven major CYP isoforms in human liver microsomes (HLMs) for evaluating the magnitude of erlotinib in drug-drug interaction in vivo. METHODS:The activities of 7 major CYP isoforms (CYP1A2,...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2010.218

    authors: Dong PP,Fang ZZ,Zhang YY,Ge GB,Mao YX,Zhu LL,Qu YQ,Li W,Wang LM,Liu CX,Yang L

    更新日期:2011-03-01 00:00:00

  • Curcumin attenuates brain edema in mice with intracerebral hemorrhage through inhibition of AQP4 and AQP9 expression.

    abstract:AIM:Aquaporins (AQPs) are the water-channels that play important roles in brain water homeostasis and in cerebral edema induced by brain injury. In this study we investigated the relationship between AQPs and a neuroprotective agent curcumin that was effective in the treatment of brain edema in mice with intracerebral ...

    journal_title:Acta pharmacologica Sinica

    pub_type: 杂志文章

    doi:10.1038/aps.2015.47

    authors: Wang BF,Cui ZW,Zhong ZH,Sun YH,Sun QF,Yang GY,Bian LG

    更新日期:2015-08-01 00:00:00