Enzyme-linked immunosorbent assay for detection of antibodies to extractable muscle antigens in myasthenia gravis.

Abstract:

:A purified citric acid soluble extract from human skeletal muscle (AEMA) and a phosphate-buffered saline extract from rabbit muscle acetone powder (EMA) were used to coat polystyrene beads in an enzyme-linked immunosorbent assay (ELISA). From 54 patients with myasthenia gravis, positive results were observed in 14. Five of 6 patients with thymoma had high levels of antibodies. With the diagnostic difficulties in detecting small and medium-sized thymoma, a sensitive assay for detection of antibodies to muscle antigen may be an important supplementary tool to detect tumors at early stages.

journal_name

Acta Neurol Scand

authors

Viana VS,Bueno C,Marchiori PE,Oliveira RM,Assis JL,Scaff M,Cossermelli W

doi

10.1111/j.1600-0404.1986.tb04606.x

subject

Has Abstract

pub_date

1986-06-01 00:00:00

pages

599-601

issue

6

eissn

0001-6314

issn

1600-0404

journal_volume

73

pub_type

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