Effect of chlorhexidine mouth rinse on Streptococci counts of tooth-tissue-borne palatal expander biofilm.

Abstract:

OBJECTIVES:To assess total Streptococci (TS) counts and biofilm mass over tooth-tissue-borne palatal expander (TTBPE), as well as the effect of chlorhexidine (CHX) mouth rinse on these variables. DESIGN:A cross-sectional study design employed clinical procedures and laboratorial techniques. SETTING AND SAMPLE POPULATION:Patients who had TTBPE removal indicated were divided into two groups: a CHX group (n = 26) in which three times a day of 0.2% CHX digluconate mouth rinses were prescribed 7 days before TTBPE removal; and a control (CON) group (n = 25) in which no antimicrobial treatment was applied. EXPERIMENTAL VARIABLE:'Gender', 'Age', and 'TTBPE wear time' were recorded. After TTBPE removal, biofilm mass was determined by the difference between (TTBPE + biofilm) and (TTBPE only) masses. TS counts were determined by biofilm suspension followed by progressive dilutions and culture on Mitis Salivarius agar with incubation at 37 degrees C for 72 h. OUTCOME MEASURE:Biofilm mass (mg) and Colony Forming Units of TS / mg of biofilm (CFU-TS / mg) were calculated. Results - Total Streptococci mean values in CHX (6.77 x 10(6)CFU-TS / mg) were statistically lower (p < 0.01) than those in CON (3.82 x 10(7)CFU-TS / mg), but there was no statistical difference (p > 0.05) between CHX (168.88 mg) and CON (182.04 mg) masses nor statistical correlation (p > 0.05) between biofilm mass and CFU-TS / mg in the two groups. CONCLUSION:Chlorhexidine reduces the TS counts in TTBPE, but has no effect on biofilm mass.

journal_name

Orthod Craniofac Res

authors

Maruo IT,Rosa EA,Maruo H,Tanaka O,Guariza Filho O,Ignácio SA,Camargo ES

doi

10.1111/j.1601-6343.2007.00418.x

subject

Has Abstract

pub_date

2008-08-01 00:00:00

pages

136-42

issue

3

eissn

1601-6335

issn

1601-6343

pii

OCR418

journal_volume

11

pub_type

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