Abstract：
:Sarcoma 180 tumor tissue from C57 mice was processed in a soft agar clonogenic assay immediately after removal from the animal and after various methods of storage. The sensitivity to the antineoplastic drug cis-platinum was not affected by different storage methods. The highest yield of colony forming cells per 100 mg tumor tissue was 2.3 X 10(4) cells following immediate processing of fresh tumor material. A 24-h storage of solid tumor tissue at 4 degrees C as well as cryopreservation of solid tumor tissue or cryopreservation of a single cell suspension prepared from fresh tumor specimens were found to reduce this value to 24%-37%. Storage of a tumor single cell suspension for 24 h at 4 degrees C however, proved to be a useful and simple method of delayed processing of tumor in a soft agar clonogenic assay. The observed cell yield was 1.91 X 10(4) colony forming cells, 83% of the immediately processed value. If this procedure is suitable for use with human tumor material obtained from biopsies and surgery, the 24 h time interval would be useful for transport from hospital to an appropriate special laboratory. This would result in the broader application of the human soft agar clonogenic assay in predictive testing and drug screening.
journal_name
J Cancer Res Clin Oncoljournal_title
Journal of cancer research and clinical oncologyauthors
Krischke W,Hartmann R,Schneider M,Schönenberger Hdoi
10.1007/BF00417832subject
Has Abstractpub_date
1988-01-01 00:00:00pages
170-6issue
2eissn
0171-5216issn
1432-1335journal_volume
114pub_type
杂志文章abstract：PURPOSE:Mucosal inflammation in the small intestine is a potentially hazardous side effect of abdominal irradiation. In an effort to develop a quantitative method of evaluating mucosal damage, the luminal release of brush border enzymes in response to ionizing radiation was examined using two investigational strategies...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s004320000174
更新日期：2001-02-01 00:00:00
abstract：:Cases of cancer notified to the Danish Cancer Registry during the period 1970 to 1984 in the age groups 16 to 66 years have been linked to information on employment kept on file in the nationwide Supplementary Pension Fund since 1964. Industrial hygienists classified industrial groups as defined by the Pension Fund wi...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF02128189
更新日期：1988-01-01 00:00:00
abstract：PURPOSE:We aimed to clarify the prognostic impact of the anatomic extent of hepatic resection (Hr) related to the tumor hepatic involvement (H) on patients' survival in the treatment of hepatocellular carcinoma (HCC). METHOD:The 305 patients with HCC who had undergone hepatectomy were analyzed retrospectively. The pat...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-007-0205-1
更新日期：2007-08-01 00:00:00
abstract：:A brain metastasis of bronchial squamous cell carcinoma in a patient was cultivated. The cells with epithelium-like characteristics were selected from the primary culture, and were subcultured to establish a cell line. The cultured cells were identified to be neoplastic by means of heterologous transplantation to nude...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00412894
更新日期：1980-01-01 00:00:00
abstract：:Phenotypically altered, preneoplastic cell populations were detected by micromorphological and cytochemical methods in a number of tissues treated with various chemical carcinogens. Further cellular analysis of carcinogenesis has shown that different cellular phenotypes follow each other during tumor development. Thus...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00390968
更新日期：1984-01-01 00:00:00
abstract：BACKGROUND:The clinical behaviour and outcome of young patients with gastroesophageal tumours (GET) is surmised to differ from older patients, yet data on the comparison of these two patient subgroups is scarce. This study focuses on the investigation of the clinical characteristics and survival outcome of younger-age ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-020-03302-x
更新日期：2020-12-01 00:00:00
abstract：PURPOSE:To study the effects of surgery and timing of radiotherapy on patient survival in grade 2 gliomas. METHODS:One hundred and eighty-nine patients with diffuse astrocytomas, oligoastrocytomas, and oligodendrogliomas, World Health Organization grade 2, treated between 1982 and 2000 were identified. The impact of t...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-003-0423-0
更新日期：2003-03-01 00:00:00
abstract：PURPOSE:Metastasis is a primary cause of colorectal cancer (CRC)-related death, and cancer stem cells (CSCs) are thought to be majorly responsible for initiating metastatic behaviors. Doublecortin-like kinase 1 (DCLK1) was recently discovered to be a marker for gastrointestinal CSCs. Here, we aimed to explore whether D...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-016-2218-0
更新日期：2016-10-01 00:00:00
abstract：PURPOSE:The aim of our study was to identify factors which influence survival in patients with disseminated seminoma in the good prognostic group according to IGCCCG, as well as to evaluate the impact of treatment intensification in patients with negative prognostic factors. METHODS:We analyzed the database of the pat...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-015-1914-5
更新日期：2015-07-01 00:00:00
abstract：PURPOSE:The incidence and mortality of gastric and colorectal cancers are among the highest malignant tumors in China. The aim of this study is to investigate whether variations of the human oxoguanine glycosylase 1 (hOGG1) gene are related to the risk of gastric and colorectal cancers in the Chinese population. METHO...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-011-1022-0
更新日期：2011-10-01 00:00:00
abstract：:This report describes two types of reticular epithelial cell in the thymic cortex of the BALB/c mouse, an immature and a mature form. During early stages of lymphoma development, i.e., 2-6 weeks postinfection (p.i.) with Moloney leukemia virus (M-MuLV), activation of the epithelial cells is observed. Although the perc...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00395387
更新日期：1983-01-01 00:00:00
abstract：PURPOSE:The oncogenic roles of sphingosine kinase 1 (SphK1) in various cancers, including thyroid cancer, have been well demonstrated. However, the microRNAs (miRNAs) associated with the oncogenic roles of SphK1 remain largely unknown. METHODS:Global gene and miRNA expression in TPC1-Vector and TPC1-SphK1 cells was an...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-016-2315-0
更新日期：2017-04-01 00:00:00
abstract：:General principles for anticancer drug development include traditional drug-screening methods in biological test systems. Today, testing of a drug in a panel of selected human tumor xenografts in mice is assumed to have the best predictive value for clinical efficacy. Chemical modification of well-known antitumor drug...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章,评审
doi：10.1007/BF01209645
更新日期：1996-01-01 00:00:00
abstract：:Two cell clones of a methylnitrosourea-induced rat mammary carcinoma, a hyperdiploid (44 chromosomes, clone A) and a hypertetraploid clone (88 chromosomes, clone B) were cultured and transplanted subcutaneously into three groups of eight rats. Group 1 was treated with 62.6 mg cyclophosphamide/kg, group 2 with 41.8 mg/...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00397925
更新日期：1989-01-01 00:00:00
abstract：PURPOSE:The spread of malignant lymph nodes due to malignancies of the head and neck is systematically observed. However, sentinel lymph nodes in the cervical region, such as in the axillary or supraclavicular regions, are not described. Therefore, precise preoperative lymph node screening of all neck compartments is r...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-008-0487-y
更新日期：2009-06-01 00:00:00
abstract：:The Harvey ras locus was examined for restriction fragment polymorphism and loss of allelic heterozygosity in 62 oral cancer patients. Southern blot analysis on BamHI digests of the tumour tissue DNA, revealed 23 patients with H-ras-1 heterozygosity. The probes used to study the polymorphism were the BamHI 6.6-kb frag...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01612771
更新日期：1991-01-01 00:00:00
abstract：PURPOSE:Adenovirus (Ads) is one of the most popular vectors used in gene therapy for the treatment of cancer. However, systemic therapy is limited by circulating antiviral antibodies and poor viral delivery in vivo. In this study, we used cytokine-induced killer (CIK) cells as delivery vehicles of Ads KGHV500 carrying ...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-019-02857-8
更新日期：2019-05-01 00:00:00
abstract：PURPOSE:Interferon alpha (IFNalpha) has been used in the immunotherapy of renal cell carcinoma (RCC), but the various mechanisms of its antiproliferative effects are poorly understood. Recent evidence suggests that IFNalpha is involved in the up-regulation of multidrug resistance (MDR) gene expression, and that the MDR...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s004320050252
更新日期：1999-01-01 00:00:00
abstract：:Butylnitrosourea (BNU) was used to induce thymic lymphomas in BDF1 mice. During and after the 12-week BNU exposure the spontaneous NK cell activity against YAC-1 cells and that arising 4 days after stimulation with Corynebacterium parvum (CP) were measured, as were the mitogen responses of splenic T and B cells. In ad...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01032607
更新日期：1984-01-01 00:00:00
abstract：PURPOSE:Promising new therapies for neoplasia include tyrosine kinase receptor antagonists. Tyrosine kinase oncogenes present an appealing anti-tumor drug target since they play an integral role in a variety of cellular responses including cell proliferation and differentiation. We previously demonstrated a high rate o...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-004-0545-z
更新日期：2004-04-01 00:00:00
abstract：PURPOSE:RhoA and RhoC are closely related, small GTPases that are clearly involved in breast cancer tumorigenesis. Nonetheless, their specific roles in the control of estrogen receptor alpha (ERα) activities have not been elucidated. METHODS:We used siRNA sequences to specifically down-regulate RhoA and RhoC expressio...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-013-1533-y
更新日期：2013-12-01 00:00:00
abstract：PURPOSE:The feasibility of radioimmunotherapy (RAIT) combined with 5-fluorouracil (5-FU) was examined in colon cancer xenografts. The mode of interaction of the two treatments was also investigated. METHODS:Mice bearing human colon cancer were treated with a combination of 4.63 MBq (L-RAIT) or 9.25 MBq (H-RAIT) (131)I...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s004320050326
更新日期：1999-11-01 00:00:00
abstract：PURPOSE:To investigate the efficacy and safety of nab-paclitaxel plus S-1 (nab-P/S) versus nab-paclitaxel plus gemcitabine (nab-P/G) as first-line chemotherapy in patients with advanced pancreatic ductal adenocarcinoma (PDAC). METHODS:Treatment-naïve patients with advanced PDAC were equally randomized to receive nab-P...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-020-03442-0
更新日期：2020-11-15 00:00:00
abstract：:The nuclear DNA content (DNA ploidy) level was determined in a series of 83 human brain metastases, for which 35 complete clinical follow-ups were available. The DNA ploidy level determination was carried out by means of DNA histogram types. The results show that certain brain metastases were diploid, while others exh...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01226271
更新日期：1996-01-01 00:00:00
abstract：:Among several nucleosides, adenosine is the only one to induce typical apoptotic cell death in human promyelocytic leukemia HL-60 cells. Intracellularly transported adenosine seemed to be required for the induction of apoptosis, since dipyridamole, which inhibits the transport of adenosine, strongly suppressed apoptos...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s004320050201
更新日期：1998-01-01 00:00:00
abstract：:The oxazaphosphorine analog ifosfamide (IFO) has demonstrated an increased therapeutic index in a variety of solid tumors and hematologic malignancies compared with its parent compound cyclophosphamide. A fractionated dose schedule over 5 days as continuous infusion in combination with the uroprotective agent sodium-2...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01613216
更新日期：1991-01-01 00:00:00
abstract：OBJECTIVE:Brain metastasis as the first symptom of lung cancer is a unique clinical entity. We conducted a retrospective study to investigate the clinical characteristics and survival of patients with lung cancer whose first symptom was brain metastases in an Asian population. METHODS:A retrospective study of 186 such...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s00432-012-1344-6
更新日期：2013-03-01 00:00:00
abstract：AIM:To study the role of beta-catenin gene mutation and expression in hepatocellular carcinogenesis. METHOD:Thirty-four hepatocellular carcinoma (HCC) specimens and adjacent para-cancerous tissues, and four normal liver tissues were analyzed. Subcellular distribution of beta-catenin was examined by immunohistochemistr...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/s004320100259
更新日期：2001-09-01 00:00:00
abstract：:Coumarin (1,2-benzopyrone) is a natural substance that has shown antitumor activity in vivo. The major human metabolite of coumarin, 7-hydroxycoumarin (7-HC), is the active form of the drug. While the exact mechanism(s) of action of coumarin is unknown, it has been shown previously that this drug possesses immunomodul...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF01377114
更新日期：1994-01-01 00:00:00
abstract：:Ascites tumor cells (2 X 10(6] of a DMBA-induced rat mammary adenocarcinoma (HH9-cl 14) were injected s.c. into tumor-free syngeneic female rats and produced a continuously growing solid tumor in all animals of this group. Inoculation of 2 X 10(7) cells induced a first brief period of tumor growth, followed by complet...
journal_title：Journal of cancer research and clinical oncology
pub_type： 杂志文章
doi：10.1007/BF00402769
更新日期：1986-01-01 00:00:00
