Analysis of the phytochrome gene family in Ceratodon purpureus by gene targeting reveals the primary phytochrome responsible for photo- and polarotropism.

Abstract:

:Using gene targeting by homologous recombination in Ceratodon purpureus, we were able to knock out four phytochrome photoreceptor genes independently and to analyze their function with respect to red light dependent phototropism, polarotropism, and chlorophyll content. The strongest phenotype was found in knock-out lines of a newly described phytochrome gene termed CpPHY4 lacking photo- and polarotropic responses at moderate fluence rates. Eliminating the atypical phytochrome gene CpPHY1, which is the only known phytochrome-like gene containing a putative C-terminal tyrosine kinase-like domain, affects red light-induced chlorophyll accumulation. This result was surprising, since no light dependent function was ever allocated to this unusual gene.

journal_name

Planta

journal_title

Planta

authors

Mittmann F,Dienstbach S,Weisert A,Forreiter C

doi

10.1007/s00425-009-0922-6

subject

Has Abstract

pub_date

2009-06-01 00:00:00

pages

27-37

issue

1

eissn

0032-0935

issn

1432-2048

journal_volume

230

pub_type

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