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A functional screen to identify novel effectors of hematopoietic stem cell activity.

Abstract：

:Despite tremendous progress made toward the identification of the molecular circuitry that governs cell fate in embryonic stem cells, genes controlling this process in the adult hematopoietic stem cell have proven to be more difficult to unmask. We now report the results of a novel gain-of-function screening approach, which identified a series of 18 nuclear factors that affect hematopoietic stem cell activity. Overexpression of ten of these factors resulted in an increased repopulating activity compared to unmanipulated cells. Interestingly, at least four of the 18 factors, Fos, Tcfec, Hmgb1, and Sfpi1, show non-cell-autonomous functions. The utilization of this screening method together with the creation of a database enriched for potential determinants of hematopoietic stem cell self-renewal will serve as a resource to uncover regulatory networks in these cells.

journal_name

Cell

journal_title

Cell

authors

Deneault E,Cellot S,Faubert A,Laverdure JP,Fréchette M,Chagraoui J,Mayotte N,Sauvageau M,Ting SB,Sauvageau G

doi

10.1016/j.cell.2009.03.026

subject

Has Abstract

pub_date

2009-04-17 00:00:00

pages

369-79

issue

2

eissn

0092-8674

issn

1097-4172

pii

S0092-8674(09)00325-0

journal_volume

137

pub_type

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