Abstract:
BACKGROUND:MicroRNAs play a crucial role in diabetic peripheral neuropathic pain (DPNP). miR-590-3p is a novel miRNA and involved in multiple diseases. However, the pathological mechanism of miR-590-3p in DPNP needs to be elucidated. MATERIALS AND METHODS:The db/db mice and db/m mice were selected to mimic diabetes and control, respectively, for in vivo studies. The miR-590-3p agomir was injected into db/db mice and pain-related behavioral tests were performed. The interaction of miR-590-3p with target gene was confirmed by dual-luciferase reporter assay. The expression of target gene was determined by qRT-PCR and western blot assay. The levels of inflammatory cytokines were measured by enzyme-linked immunosorbent assay (ELISA). RESULTS:miR-590-3p was down-regulated in diabetic peripheral neuropathy mice. More importantly, miR-590-3p agomir alleviated pain-related behavior, reduced TNF-α, IL-1β and IL-6 concentrations, and inhibited neural infiltration by immune cells in db/db mice. Interestingly, RAP1A was predicted to be the target of miR-590-3p by Targetscan, and was actually regulated by miR-590-3p. Finally, the rescue experiments proved that overexpression of RAP1A partially abrogated the suppressive impact of miR-590-3p on T cells proliferation and migration. CONCLUSION:miR-590-3p ameliorates DPNP via targeting RAP1A and inhibiting T cells infiltration, indicating that exogenous miR-590-3p may be a potential candidate for clinical treatment of DPNP.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Wu Y,Gu Y,Shi Bdoi
10.18388/abp.2020_5451subject
Has Abstractpub_date
2020-12-17 00:00:00pages
587-593issue
4eissn
0001-527Xissn
1734-154Xpii
5451journal_volume
67pub_type
杂志文章abstract::The content and type of dolichols in chicken embryo fibroblasts was estimated and the presence of enzymic activities of various dolichyl phosphate sugar transferases was documented. Chicken embryo fibroblasts effectively take up various polyprenoids from the culture medium and catalyse both formation of polyprenyl fat...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00
abstract::The aim of this study was to investigate the antimicrobial effect of lipopeptide biosurfactants from surfactin, iturin and fengycin families, synthesised by the Bacillus subtilis I'1a strain, on uropathogenic bacteria, including the effects on planktonic growth, processes of biofilm formation and dislodging. Antimicro...
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doi:10.18388/abp.2015_1120
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abstract::In this study we investigated the level of 8-oxo-2'-deoxyguanosine (8-oxodG) in DNA of Cardamine pratensis plants subjected to different growth conditions trying to answer the question whether factors like light and water accessibility or low temperature may have an impact on the total DNA oxidative damage. The level ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::This article reviews the evidence for the danger model in the context of immune response to tumors and the insufficiency of the immune system to eliminate tumor growth. Despite their potential immunogenicity tumors do not induce significant immune responses which could destroy malignant cells. According to the danger ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
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abstract::Ammonium salt of N-(dithiocarboxy)sarcosine (DTCS) chelated to ferrous salt was tested as an NO-metric spin trap at room temperature for ex vivo measurement of (.)NO production in murine endotoxaemia. In a chemically defined in vitro model system EPR triplet signals of NO-Fe(DTCS)(2) were observed for as long as 3 hou...
journal_title:Acta biochimica Polonica
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更新日期:2003-01-01 00:00:00
abstract::We assessed a relationship between the level of 8-oxodG in leukocyte DNA measured with the high performance liquid chromatography with electrochemical detection (HPLC/EC) technique and urinary excretion of the modified nucleoside/base analysed with a recently developed methodology involving HPLC prepurification follow...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:035002549
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abstract::The presence of the single metals (Cd, Pb, Cu, Zn) induces ROS (reactive oxygen species) production and causes oxidative stress in plants. While applied in two-element combinations, trace metals impact organisms in a more complex way. To assess the resultant effect we treated the pea grown hydroponically with the trac...
journal_title:Acta biochimica Polonica
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doi:
更新日期:2014-01-01 00:00:00
abstract::The mammalian deoxyribonucleoside kinases thymidine kinase 1 and 2, deoxycytidine kinase and deoxyguanosine kinase phosphorylate deoxyribonucleosides and provide an alternative to de novo synthesis of DNA precursors. Their activities are essential for activation of several chemotherapeutically important nucleoside ana...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract::Aims. This study aimed to investigate CHRNA3 (rs8040868) and PHACTR2 (rs9390123) single-nucleotide polymorphisms (SNPs) for association with non-small-cell lung cancer (NSCLC) risk in a Chinese population, and whether the environment affects the genetic polymorphisms. Methods. This case and control study included 500 ...
journal_title:Acta biochimica Polonica
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doi:
更新日期:2014-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2016_1369
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abstract::The aim of this study was to compare the content of carotenoids in seventeen cultivars of carrots grown in Poland. Conventional orange cultivars with rarely grown were compared: white, yellow and purple with yellow core cultivars. To determine the content of carotenoids, extracts from lyophilized carrot roots were pre...
journal_title:Acta biochimica Polonica
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doi:
更新日期:2012-01-01 00:00:00
abstract::The root system of rice is influenced by various environmental factors. However, how the root system responds to abiotic stress has not yet been fully understood. A zinc finger protein gene, OsZFP350, is exclusively expressed in the rice root, but its biological function needs to be investigated. Expression of OsZFP35...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2018_2765
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:0249041029
更新日期:2002-01-01 00:00:00
abstract::In the present work, we have reviewed data showing that triiodothyronine and its nuclear receptors modify expression of different genes/proteins involved in cell cycle control beginning from growth factors (such as EGF and TGF-beta), to cell surface receptors (EGFR), as well as proteins acting at the cell membrane (Ra...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
abstract::In the haemolymph of Oniscidea haemocyanin exists mainly in the form of hexamer 16S (90%); the 5S and 24S components are present in small amounts. Alkaline dissociation of the examined 16S haemocyanins is dependent on pH and divalent cations (Ca2+,Mg2+). The 16S component consists of 23-24.5% acidic amino acids, 15-18...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Nanostructured lipid carriers (NLC) are stable colloidal formulations with notable advantages for drug delivery systems. Thanks to their physicochemical stability, biocompatibility, biodegradability and controlled drug release, they have received increasing attention for the last several years. The aim of the study wa...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Since the discovery of the first E. coli mutator gene, mutT, most of the mutations inducing elevated spontaneous mutation rates could be clearly attributed to defects in DNA repair. MutT turned out to be a pyrophosphohydrolase hydrolyzing 8-oxodGTP, thus preventing its incorporation into DNA and suppresing the occurre...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2007-01-01 00:00:00
abstract::The integration host factor (IHF) is a sequence-specific, histone-like, multi-functional DNA-binding and -bending protein of Escherichia coli. Characterization and functional analysis of this protein has been carried out mainly in bacteriophage lambda and other mobile genetic elements. In this paper we report data con...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::alpha-Glycerophosphate dehydrogenase (EC 1.1.99.5) in mitochondria from liver of the triiodothyronine-treated rats is competitively inhibited by phosphoenolpyruvate, glyceraldehyde 3-phosphate and 3-phosphoglycerate, the apparent Ki values for phosphoenolpyruvate being 0.76 mM at pH 7.0, 1.7 mM at pH 7.4 and 3.5 mM at...
journal_title:Acta biochimica Polonica
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doi:
更新日期:1977-01-01 00:00:00
abstract::X-ray crystallography provides important insights into structure-function relationship in biomolecules. However, protein crystals are usually hard to obtain which hinders our understanding of multiple important processes. Crystallization requires large amount of protein sample, whereas recombinant proteins are often u...
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pub_type: 杂志文章
doi:10.18388/abp.2015_1026
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journal_title:Acta biochimica Polonica
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doi:
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journal_title:Acta biochimica Polonica
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doi:
更新日期:2007-01-01 00:00:00
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doi:10.18388/abp.2015_1113
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更新日期:2009-01-01 00:00:00
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更新日期:2012-01-01 00:00:00
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journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1985-01-01 00:00:00