Column chromatography as a useful step in purification of diatom pigments.

abstract：:Yeasts are a convenient platform for many applications. They have been widely used as the expression hosts. There is a need to have a new yeast expression system to contribute the molecular cloning demands. Eight yeast isolates were screened from various environment sources and identified through ribosomal DNA (rDNA) ...

journal_title：Acta biochimica Polonica

authors： Oslan SN,Salleh AB,Rahman RN,Basri M,Chor AL

更新日期：2012-01-01 00:00:00

abstract：:1. The amino acid composition of wheat germ EF2 differs to some extent from that of elongation factors from mammals and bacteria. 2. The purified wheat germ EF2, similarly as the factors from other sources, is active in the: EF1-dependent polymerization of phenylalanine; ribosome-dependent GTP hydrolysis; binding of g...

journal_title：Acta biochimica Polonica

authors： Twardowski T,Legocki A

更新日期：1977-01-01 00:00:00

abstract：:Cardiac fibrosis is referred to as an excessive accumulation of stromal cells and extracellular matrix proteins in the myocardium. Progressive fibrosis causes stiffening of the cardiac tissue and affects conduction of electrical impulses, leading to heart failures in a broad range of cardiac conditions. At the cellula...

journal_title：Acta biochimica Polonica

authors： Działo E,Tkacz K,Błyszczuk P

更新日期：2018-01-01 00:00:00

abstract：:The importance of nosocomial infections caused by coagulase-negative staphylococci is constantly growing. The threat primarily affects immunocompromised patients, the elderly and neonates, particularly after invasive surgery. The problem is fundamentally exacerbated by expanding antibacterial drug resistance. A case r...

journal_title：Acta biochimica Polonica

authors： Savini V,Kosecka M,Siegwart E,Marrollo R,Polilli E,Palmieri D,Fazii P,Carretto E,Międzobrodzki J,Bukowski M

更新日期：2016-01-01 00:00:00

abstract：:Immunoglobulins anti-endonuclease 32 kDa inhibit DNA synthesis. We observed that low concentrations of IgGs (about 50 micrograms IgG per 1 x 10(6) cell nuclei) temporary inhibit DNA synthesis. This inhibition concerns only the synthesis of DNA bound to the nuclear matrix (associated with isolated nuclear matrix). Prei...

journal_title：Acta biochimica Polonica

authors： Rzepecki R,Szopa J

更新日期：1995-01-01 00:00:00

abstract：:Changes in the composition of fatty acids (FA) were determined in lipid extracts isolated from developing ovaries containing ovules and developing seeds of Echium vulgare L. The samples were collected successively over 20 days beginning with the first day after flowering. The contents of the n-6 FA family members, i.e...

journal_title：Acta biochimica Polonica

authors： Stolyhwo A,Mol J

更新日期：2007-01-01 00:00:00

abstract：:Transcriptionally active and inactive chromatin fractions were isolated from Physarum polycephalum after depolymerization of chromatin with DNAase II or micrococcal nuclease, followed by fractionation in 5 mM-MgCl2. The active fraction of chromatin comprised up to 21% of nuclear DNA and was enriched 22-fold in the lab...

journal_title：Acta biochimica Polonica

authors： Czupryn M,Toczko K

更新日期：1980-01-01 00:00:00

abstract：:In contrast to most other eukaryotic organisms, yeast can survive without respiration. This ability has been exploited to investigate nuclear genes required for expression of mitochondrial DNA. Availability of complete Saccharomyces cerevisiae genomic sequence has provided additional help in detailed molecular analysi...

journal_title：Acta biochimica Polonica

authors： Chacińska A,Boguta M

更新日期：2000-01-01 00:00:00

abstract：:The aim of this study was to determine the level of antibodies against hemagglutinin of influenza viruses in the sera of people in the seven age groups in the epidemic season 2018/2019 in Poland. The level of anti-hemagglutinin antibodies was determined by hemagglutination inhibition test (HAI). 1050 clinical samples ...

journal_title：Acta biochimica Polonica

authors： Hallmann-Szelińska E,Szymański K,Łuniewska K,Kondratiuk K,Brydak LB

更新日期：2020-03-17 00:00:00

abstract：:The present study was undertaken to evaluate the protective effects of Linum usitatissimum oil (LuO) against sub-chronic Roundup (RDP)-induced toxicity and oxidative stress in rats. Rats were divided into four groups: control group (no treatment), RDP group (Roundup at 269.9 mg/kg b.w.), LuO group (0.5 g/kg b.w. of Lu...

journal_title：Acta biochimica Polonica

authors： Djaber N,Ounaceur LS,Moubine BN,Khaldi T,Rouag M,Berrouague S,Amara H,Taibi F,Boumendjel M,Boumendjel A,Messarah M

更新日期：2020-03-18 00:00:00

abstract：:A conservative and apparently harmless A176V mutation in intracellular S. cerevisiae L-asparaginase (ScerAI) completely abolishes the enzyme activity. Sequence and structural comparisons with type II bacterial L-asparaginases show that the mutated residue is in a very conservative region and plays a vital role in the ...

journal_title：Acta biochimica Polonica

authors： Bonthron DT,Jaskólski M

更新日期：1997-01-01 00:00:00

abstract：:The mammalian deoxyribonucleoside kinases thymidine kinase 1 and 2, deoxycytidine kinase and deoxyguanosine kinase phosphorylate deoxyribonucleosides and provide an alternative to de novo synthesis of DNA precursors. Their activities are essential for activation of several chemotherapeutically important nucleoside ana...

journal_title：Acta biochimica Polonica

authors： Johansson NG,Eriksson S

更新日期：1996-01-01 00:00:00

abstract：:In the haemolymph of Oniscidea haemocyanin exists mainly in the form of hexamer 16S (90%); the 5S and 24S components are present in small amounts. Alkaline dissociation of the examined 16S haemocyanins is dependent on pH and divalent cations (Ca2+,Mg2+). The 16S component consists of 23-24.5% acidic amino acids, 15-18...

journal_title：Acta biochimica Polonica

authors： Helszer Z,Gondko R

更新日期：1991-01-01 00:00:00

abstract：:Clofarabine (2-chloro-2'-fluoro-2'-deoxyarabinosyladenine, ClF), a second-generation 2'-deoxyadenosine analog, possesses manifold anti-cancer activities. Our previous reports and some of others demonstrate the potential capacity of ClF to regulate the epigenetic machinery. The study presented here is the first to inve...

journal_title：Acta biochimica Polonica

authors： Kaufman-Szymczyk A,Lubecka K

更新日期：2020-01-30 00:00:00

abstract：:Triterpene saponins (saponosides) are found in a variety of higher plants and display a wide range of pharmacological activities, including expectorant, anti-inflamatory, vasoprotective, gastroprotective and antimicrobial properties. Recently, a potential anticancer activity of saponins has been suggested by their cyt...

journal_title：Acta biochimica Polonica

authors： Koczurkiewicz P,Czyż J,Podolak I,Wójcik K,Galanty A,Janeczko Z,Michalik M

更新日期：2015-01-01 00:00:00

abstract：:The phosphatidylinositol-3-kinases (PtdIns-3-kinase) are a family of enzymes involved in the control of cell replication. One member of the family, the mammalian p110/p85 PtdIns-3-kinase, is a potential target for anticancer drug development because of its role as a component of growth factor and oncogene activated si...

journal_title：Acta biochimica Polonica

authors： Powis G,Berggren M,Gallegos A,Frew T,Hill S,Kozikowski A,Bonjouklian R,Zalkow L,Abraham R,Ashendel C

更新日期：1995-01-01 00:00:00

abstract：:Nanostructured lipid carriers (NLC) are stable colloidal formulations with notable advantages for drug delivery systems. Thanks to their physicochemical stability, biocompatibility, biodegradability and controlled drug release, they have received increasing attention for the last several years. The aim of the study wa...

journal_title：Acta biochimica Polonica

authors： Lasoń E,Sikora E,Ogonowski J

更新日期：2013-01-01 00:00:00

abstract：:Glutathione-S-transferase (GST) a Phase-II drug detoxification enzyme, was detected in Setaria cervi, a bovine filarial parasite. In vitro effect of diethylcarbamazine, butylated hydroxyanisole and phenobarbitone on the GST of adult female S. cervi was assayed by the addition of these compounds in the maintenance medi...

journal_title：Acta biochimica Polonica

authors： Gupta S,Rathaur S

更新日期：2005-01-01 00:00:00

abstract：:The aim of this study was to conduct an epidemiological and laboratory surveillance of Influenza-Like Illnesses (ILI) in Polish Armed Forces, civilian military personnel and their families in 2011/2012 epidemic season, under the United States Department of Defense-Global Emerging Infections Surveillance and Response S...

journal_title：Acta biochimica Polonica

authors： Kocik J,Niemcewicz M,Winnicka I,Michalski A,Bielawska-Drózd A,Kołodziej M,Joniec J,Cieślik P,Graniak G,Mirski T,Gaweł J,Bielecka-Oder A,Kubiak L,Russell K

更新日期：2014-01-01 00:00:00

abstract：:The SOS-Chromotest was used to detect DNA damage induced by two flavones: apigenin and luteolin and/or their metabolites. It was found that the compounds tested weakly induced the SOS repair system in the strain studied. ...

journal_title：Acta biochimica Polonica

authors： Czeczot H,Bilbin M

更新日期：1991-01-01 00:00:00

abstract：:The aim of the study presented here was an attempt to answer the question posed in the title: Is the mechanism of nitroglycerin tolerance associated with aldehyde dehydrogenase (ALDH) activity? Here, we investigated the effect of administration (separately or jointly) of lipoic acid (LA), nitroglycerin (GTN), and disu...

journal_title：Acta biochimica Polonica

authors： Bilska-Wilkosz A,Kotańska M,Górny M,Filipek B,Iciek M

更新日期：2019-12-28 00:00:00

abstract：:The base analogue 6-N-hydroxylaminopurine (HAP) is a potent mutagen in a variety of prokaryotic and eukaryotic organisms. Mutations in the yeast ham1 gene render the cells hypersensitive to the mutagenic effect of HAP. We have found that this gene has homologues in a variety of organisms from bacteria to man. We have ...

journal_title：Acta biochimica Polonica

authors： Kozmin SG,Leroy P,Pavlov YI

更新日期：1998-01-01 00:00:00

abstract：:Uracil-DNA glycosylase of Archaeoglobus fulgidus (Afung) in cell extracts exhibited maximal activity around pH 6.2 as compared to pH 4.8 for the purified recombinant enzyme expressed in Escherichia coli. Native Afung thus seems to be adapted to the intracellular pH of A. fulgidus, determined to be 7.0±0.1. Both recomb...

journal_title：Acta biochimica Polonica

authors： Knævelsrud I,Kazazic S,Birkeland NK,Bjelland S

更新日期：2014-01-01 00:00:00

abstract：:Syntheses of biomimetic low-molecular weight poly-(R)-3-hydroxybutanoate mediated by three types of supramolecular catalysts are presented. The utility of these synthetic polyesters for preparation of artificial channels in phospholipid bilayers capable of sodium and calcium ion transport across cell membranes, is dis...

journal_title：Acta biochimica Polonica

authors： Jedliński Z,Kurcok P,Adamus G,Juzwa M

更新日期：2000-01-01 00:00:00

abstract：:Recently, embryo muscle development, which is crucial for postnatal skeletal muscle growth, has been investigated widely. Nutrients in ovo were suggested to be critical in embryo muscle development since the chick growth mostly relies on nutrients in eggs at the early developmental stage. Phytosterol esters (PE), whic...

journal_title：Acta biochimica Polonica

authors： Wang L,Zuo X,Zhao W,Zhou G,Luo L,Yang K,Shu G,Wang S,Gao P,Zhu X,Jiang Q,Wang L

更新日期：2020-03-19 00:00:00

abstract：:Ovine β‑lactoglobulin was characterized by spectroscopic (CD), calorimetric (ITC) and X-ray structural studies. The structure of ovine β‑lactoglobulin complex with decanol showed that tight packing of molecules in the crystalline phase enforces a distortion of protein flexible loops resulting in the formation of an as...

journal_title：Acta biochimica Polonica

authors： Loch J,Bonarek P,Lewiński K

更新日期：2019-12-27 00:00:00

abstract：:Little is known about the molecular background of clinical variability of Charcot-Marie-Tooth type 1A (CMT1A) disease and hereditary neuropathy with liability to pressure palsies (HNPP). The CMT1A and HNPP disorders result from duplication and deletion of the PMP22 gene respectively. In a series of studies performed o...

journal_title：Acta biochimica Polonica

authors： Sinkiewicz-Darol E,Kabzińska D,Moszyńska I,Kochański A

更新日期：2010-01-01 00:00:00

abstract：:We here report the influence of the cell cycle abrogator UCN-01 on RKO human colon carcinoma cells differing in p53 status following exposure to two DNA damaging agents, the topoisomerase inhibitors etoposide and camptothecin. Cells were treated with the two drugs at the IC90 concentration for 24 h followed by post-in...

journal_title：Acta biochimica Polonica

authors： Bozko P,Larsen AK,Raymond E,Skladanowski A

更新日期：2002-01-01 00:00:00

abstract：:The evidence on the crucial role of a family of calcium-dependent cysteine proteases called calpains in programmed cell death is rich and still growing. However, understanding of the mechanisms of their functions in apoptosis is not full yet. Calpains have been implicated in both physiological and pathological cell de...

journal_title：Acta biochimica Polonica

authors： Łopatniuk P,Witkowski JM

更新日期：2011-01-01 00:00:00

abstract：:Sea urchin gonad (roe) is a highly valued food in Japan and North America. Gonad price is strongly influenced by quality, with appearance, especially colour being a major determinant. Previous attempts to extract a carotenoid profile from the New Zealand sea urchin species Evechinus chloroticus have been challenging d...

journal_title：Acta biochimica Polonica

authors： Garama D,Bremer P,Carne A

更新日期：2012-01-01 00:00:00