Abstract:
:The use of appropriate fluorescent proteins has allowed the use of FRET microscopy for investigation of intermolecular interactions in living cells. This method has the advantage of both being dynamic and of working at the subcellular level, so that the time and place where proteins interact can be visualized. We have used FRET microscopy to analyze the interactions between the T cell antigen receptor and the coreceptors CD4 and CD8. This chapter reviews data on how these coreceptors are recruited to the immunological synapse, and how they interact when the T cell is stimulated by different ligands.
journal_name
Curr Top Microbiol Immunoljournal_title
Current topics in microbiology and immunologyauthors
Gascoigne NR,Ampudia J,Clamme JP,Fu G,Lotz C,Mallaun M,Niederberger N,Palmer E,Rybakin V,Yachi PP,Zal Tdoi
10.1007/978-3-540-93864-4_2subject
Has Abstractpub_date
2009-01-01 00:00:00pages
31-46eissn
0070-217Xissn
2196-9965journal_volume
334pub_type
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