Abstract：
:A major barrier to the development of a clinically useful small diameter tissue engineered vascular graft (TEVG) is the scaffold component. Scaffold requirements include matching the mechanical and structural properties with those of native vessels and optimizing the microenvironment to foster cell integration, adhesion and growth. We have developed a small diameter, bilayered, biodegradable, elastomeric scaffold based on a synthetic, biodegradable elastomer. The scaffold incorporates a highly porous inner layer, allowing cell integration and growth, and an external, fibrous reinforcing layer deposited by electrospinning. Scaffold morphology and mechanical properties were assessed, quantified and compared with those of native vessels. Scaffolds were then seeded with adult stem cells using a rotational vacuum seeding device to obtain a TEVG, cultured under dynamic conditions for 7 days and evaluated for cellularity. The scaffold showed firm integration of the two polymeric layers with no delamination. Mechanical properties were physiologically consistent, showing anisotropy, an elastic modulus (1.4 + or - 0.4 MPa) and an ultimate tensile stress (8.3 + or - 1.7 MPa) comparable with native vessels. The compliance and suture retention forces were 4.6 + or - 0.5 x 10(-4) mmHg(-1) and 3.4 + or - 0.3N, respectively. Seeding resulted in a rapid, uniform, bulk integration of cells, with a seeding efficiency of 92 + or - 1%. The scaffolds maintained a high level of cellular density throughout dynamic culture. This approach, combining artery-like mechanical properties and a rapid and efficient cellularization, might contribute to the future clinical translation of TEVGs.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Soletti L,Hong Y,Guan J,Stankus JJ,El-Kurdi MS,Wagner WR,Vorp DAdoi
10.1016/j.actbio.2009.06.026subject
Has Abstractpub_date
2010-01-01 00:00:00pages
110-22issue
1eissn
1742-7061issn
1878-7568pii
S1742-7061(09)00278-5journal_volume
6pub_type
杂志文章abstract：:Postoperative adhesion is a serious complication that can further lead to morbidity and/or mortality. Polymer anti-adhesion barrier material provides an effective precaution to reduce the probability of postoperative adhesion. Clinical application requires these materials to be easily handled, biocompatible, biodegrad...
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journal_title：Acta biomaterialia
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doi：10.1016/j.actbio.2009.04.037
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2020-04-01 00:00:00
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更新日期：2010-07-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.actbio.2011.04.003
更新日期：2011-07-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2018.08.006
更新日期：2018-09-15 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2013.09.017
更新日期：2014-01-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2010.12.012
更新日期：2011-04-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
doi：10.1016/j.actbio.2005.07.010
更新日期：2005-11-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 临床试验,杂志文章
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更新日期：2008-03-01 00:00:00
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更新日期：2007-07-01 00:00:00
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更新日期：2021-01-01 00:00:00
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更新日期：2014-12-01 00:00:00
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更新日期：2014-08-01 00:00:00
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更新日期：2015-07-01 00:00:00
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更新日期：2011-01-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2014-07-01 00:00:00
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pub_type： 杂志文章
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更新日期：2014-10-01 00:00:00
