Abstract:
:Cell transplantation therapies to treat diseases related to dysfunction of retinal ganglion cells (RGCs) are limited in part by an inability to navigate to the optic nerve head within the retina. During development, RGCs are guided by a series of neurotrophic factors and guidance cues; however, these factors and their receptors on the RGCs are developmentally regulated and often not expressed during adulthood. Netrin-1 is a guidance factor capable of guiding RGCs in culture and relevant to guiding RGC axons toward the optic nerve head in vivo. Here we immobilized Netrin-1 using UV-initiated crosslinking to form a gradient capable of guiding the axonal growth of RGCs on a radial electrospun scaffold. Netrin-gradient scaffolds promoted both the percentage of RGCs polarized with a single axon, and also the percentage of cells polarized toward the scaffold center, from 31% to 52%. Thus, an immobilized protein gradient on a radial electrospun scaffold increases RGC axon growth in a direction consistent with developmental optic nerve head guidance, and may prove beneficial for use in cell transplant therapies for the treatment of glaucoma and other optic neuropathies.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Kador KE,Alsehli HS,Zindell AN,Lau LW,Andreopoulos FM,Watson BD,Goldberg JLdoi
10.1016/j.actbio.2014.08.032subject
Has Abstractpub_date
2014-12-01 00:00:00pages
4939-4946issue
12eissn
1742-7061issn
1878-7568pii
S1742-7061(14)00377-8journal_volume
10pub_type
杂志文章abstract:UNLABELLED:Recent studies reveal many biomaterial vaccine carriers are able to activate immunostimulatory pathways, even in the absence of other immune signals. How the changing properties of polymers during biodegradation impact this intrinsic immunogenicity is not well studied, yet this information could contribute t...
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abstract::Polylactide (PLA) polymers containing five distinct lengths of fluorinated (from C3F7 to C13F27) and non-fluorinated (C6H13) end-groups were successfully synthesized by ring-opening polymerization of d,l-lactide. Fluorination was expected to increase the encapsulation efficiency of perfluorohexane (PFH). 150 nm nanoca...
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abstract::The effect of calcium phosphate-based microchannels on the growth and orientation of human osteoblast cells is investigated in this study. As substrates, hydroxyapatite-based microchannels with high contouring accuracy were fabricated by a novel micro-moulding technique. Microchannels obtained by this method featured ...
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pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2014-10-01 00:00:00
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journal_title:Acta biomaterialia
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pub_type: 杂志文章
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更新日期:2010-07-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Acta biomaterialia
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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更新日期:2008-05-01 00:00:00
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journal_title:Acta biomaterialia
pub_type: 杂志文章
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journal_title:Acta biomaterialia
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