Abstract：
:Standard reconstructive procedures for restoring normal function after skeletal muscle defects involve the use of existing host tissues such as muscular flaps. In many instances, this approach is not feasible and delays the rehabilitation process and restoration of tissue function. Currently, cell-based tissue engineering strategies have been used for reconstruction; however, donor tissue biopsy and ex vivo cell manipulation are required prior to implantation. The present study aimed to overcome these limitations by demonstrating mobilization of muscle cells into a target-specific site for in situ muscle regeneration. First, we investigated whether host muscle cells could be mobilized into an implanted scaffold. Poly(l-lactic acid) (PLLA) scaffolds were implanted in the tibialis anterior (TA) muscle of rats, and the retrieved scaffolds were characterized by examining host cell infiltration in the scaffolds. The host cell infiltrates, including Pax7+ cells, gradually increased with time. Second, we demonstrated that host muscle cells could be enriched by a myogenic factor released from the scaffolds. Gelatin-based scaffolds containing a myogenic factor were implanted in the TA muscle of rats, and the Pax7+ cell infiltration and newly formed muscle fibers were examined. By the second week after implantation, the Pax7+ cell infiltrates and muscle formation were significantly accelerated within the scaffolds containing insulin-like growth factor 1 (IGF-1). Our data suggest an ability of host stem cells to be recruited into the scaffolds with the capability of differentiating to muscle cells. In addition, the myogenic factor effectively promoted host cell recruitment, which resulted in accelerating muscle regeneration in situ.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Ju YM,Atala A,Yoo JJ,Lee SJdoi
10.1016/j.actbio.2014.06.022subject
Has Abstractpub_date
2014-10-01 00:00:00pages
4332-9issue
10eissn
1742-7061issn
1878-7568pii
S1742-7061(14)00269-4journal_volume
10pub_type
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journal_title：Acta biomaterialia
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journal_title：Acta biomaterialia
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2013-12-01 00:00:00
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journal_title：Acta biomaterialia
pub_type： 杂志文章
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更新日期：2017-07-15 00:00:00
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更新日期：2017-02-01 00:00:00
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journal_title：Acta biomaterialia
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更新日期：2012-03-01 00:00:00
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