Abstract:
:The effect of calcium phosphate-based microchannels on the growth and orientation of human osteoblast cells is investigated in this study. As substrates, hydroxyapatite-based microchannels with high contouring accuracy were fabricated by a novel micro-moulding technique. Microchannels obtained by this method featured widths ranging from 16.0±0.7 to 76.6±1.4 μm and depths from 7.9±0.8 to 15.5±1.3 μm. Surface and contour characterization was carried out using X-ray diffraction analysis, scanning electron microscopy imaging and 3D-confocal profilometry. Cell activity and alignment on microchannels with different widths were determined after 1 and 3 days by photometric spectroscopy and fluorescence microscopic imaging and statistically analysed by Tukey's multiple comparison test. On days 1 and 3 for microchannels of width 16 and 30 μm, 70-80% of the osteoblasts oriented within an angular range of 0-15° relative to the microchannel direction. Interestingly, only 20% of the cells grew inside the microchannels for channel widths of 16 and 30 μm. Substrates with channel widths of 45, 65 and 76 μm allowed ∼40% of the cells to grow inside. The depth of the microchannel showed hardly any significant impact. All micropatterned surfaces provoked a good cell attachment, as flat and spread cell morphologies with lamellipodiae and filopodiae could already be observed after 1 day. The effect of the microchannels on osteoblast activity was determined using the colorimetric WST-1 assay. In addition, the cell differentiation was assessed by collagen type I staining. The cell activity obtained by WST-1 assay differed insignificantly for all micropatterned samples of various widths and depths. The assessment of collagen type I yielded the same amounts for all micropatterned samples after 1, 3 and 7 days. In summary, the microchannel width of HA-based patterns has a distinct effect on the directed growth of human osteoblast cells, allowing novel design strategies for surfaces such as dental implants.
journal_name
Acta Biomaterjournal_title
Acta biomaterialiaauthors
Holthaus MG,Stolle J,Treccani L,Rezwan Kdoi
10.1016/j.actbio.2011.07.031subject
Has Abstractpub_date
2012-01-01 00:00:00pages
394-403issue
1eissn
1742-7061issn
1878-7568pii
S1742-7061(11)00340-0journal_volume
8pub_type
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更新日期:2014-10-01 00:00:00
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doi:10.1016/j.actbio.2007.12.004
更新日期:2008-05-01 00:00:00
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journal_title:Acta biomaterialia
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更新日期:2014-07-01 00:00:00
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更新日期:2005-09-01 00:00:00
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