Echovirus 30 associated with cases of aseptic meningitis in state of Pará, Northern Brazil.

Abstract:

:Investigation of the aetiology of viral meningitis in Brazil is most often restricted to cases that occur in the Southern and Southeastern Regions; therefore, the purpose of this study is to describe the viral meningitis cases that occurred in state of Pará, Northern Brazil, from January 2005-December 2006. The detection of enterovirus (EV) in cerebrospinal fluid was performed using cell culture techniques, RT-PCR, nested PCR and nucleotide sequencing. The ages of the 91 patients ranged from < one year old to > 60 years old (median age 15.90 years). Fever (87.1%), headache (77.0%), vomiting (61.5%) and stiffness (61.5%) were the most frequent symptoms. Of 91 samples analyzed, 18 (19.8%) were positive for EV. Twelve were detected only by RT- PCR followed by nested PCR, whereas six were found by both cell culture and RT-PCR. From the last group, five were sequenced and classified as echovirus 30 (Echo 30). Phylogenetic analyses revealed that Echo 30 detected in Northern Brazil clustered within a unique group with a bootstrap value of 100% and could constitute a new subgroup (4c) according to the phylogenetic tree described by Oberste et al. (1999). This study described the first molecular characterization of Echo 30 in Brazil and this will certainly contribute to future molecular analyses involving strains detected in other regions of Brazil.

journal_name

Mem Inst Oswaldo Cruz

authors

Castro CM,Oliveira DS,Macedo O,Lima MJ,Santana MB,Wanzeller AL,Silveira Ed,Gomes Mde L

doi

10.1590/s0074-02762009000300008

subject

Has Abstract

pub_date

2009-05-01 00:00:00

pages

444-50

issue

3

eissn

0074-0276

issn

1678-8060

pii

S0074-02762009000300008

journal_volume

104

pub_type

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