Abstract：
:Zinc uptake regulator (Zur) is a negative transcriptional regulator of bacteria that belongs to the FUR superfamily of proteins and regulates zinc (Zn) homeostasis under extreme Zn conditions. The Zur protein of Bacillus anthracis (BaZur) was though characterized previously, but the residues of this transcriptional regulator, crucial for binding to the consensus Zur box in the cognate DNA, remain unexplored. In this study, we reveal the essential residues of the protein that govern the specific interaction with the cognate DNA, through mutational and binding studies. In silico predicted model of the BaZur protein with the promoter region of one of the regulon candidates was utilized to identify specific residues of the N-terminal domain (NTD), constituting the DNA-binding recognition helix. Our results suggest that two phenylalanine residues, a non-polar aliphatic leucine and a positively charged arginine residue of NTD, are predominantly involved in DNA binding of BaZur. Among these, the arginine residue (Arg58) is conserved among all the Zur proteins and the two Phe residues, namely Phe53 and Phe63, are conserved in the Zur proteins of Staphylococcus aureus and Listeria monocytogenes. Taken together, the current study represents an in-depth investigation into the key DNA-binding residues involved in the BaZur-DNA interaction.
journal_name
Biol Trace Elem Resjournal_title
Biological trace element researchauthors
Kandari D,Joshi H,Tanwar N,Munde M,Bhatnagar Rdoi
10.1007/s12011-020-02427-xsubject
Has Abstractpub_date
2020-10-14 00:00:00eissn
0163-4984issn
1559-0720pii
10.1007/s12011-020-02427-xpub_type
杂志文章abstract：:A study was conducted to validate the effects of nano form of zinc (NZn) on nutrient digestibility, zinc retention, organ and serum zinc profile, and hepatic metallothionein gene expression in Wistar albino rats (WAR). Nano zinc (NZn) was synthesized through chemical method, by using 0.45 M zinc nitrate [Zn(NO3)2.6H2O...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-018-1517-5
更新日期：2019-07-01 00:00:00
abstract：:Selenium is a trace element that has antioxidant and neuroprotective effects. The aim of this study is to investigate the effects of selenium in reducing ischemia-reperfusion injury of the gastrocnemius muscle. In this experimental study, 80 adult male Wistar rats weighing 250-300 g were divided into ten groups (N = 8...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-0218-y
更新日期：2015-04-01 00:00:00
abstract：:The effects of environmental lead on the immune system of young children were assessed by determining the peripheral blood lymphocytes CD3+, CD4+, CD8+, B(CD19+) counts, and natural killer (CD16+CD56+) cells in 35 preschool children whose mean blood lead level was 140.6 microg/L. The results were compared to an age- a...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:105:1-3:019
更新日期：2005-07-01 00:00:00
abstract：:Lead poisoning is a worldwide health problem, and its treatment is under investigation. The aim of this study was to access the efficacy of Coriandrum sativum (coriander) in reducing lead-induced changes in mice testis. Animal exposed to lead nitrate showed significant decrease in testicular SOD, CAT, GSH, total prote...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-009-8553-0
更新日期：2010-09-01 00:00:00
abstract：:The aim of this study was to assess the effect of sibutramine on mineral status and selected biochemical parameters in obese women. The study was conducted on 24 patients who received 15 mg daily doses of sibutramine for 12 weeks, and on 20 patients who received placebo. At the baseline, after the sixth and twelfth we...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-012-9425-6
更新日期：2012-11-01 00:00:00
abstract：:To elucidate the element content of newborn blood vessels, umbilical arteries and veins in human umbilical cords, which had the advantage of easy sampling, were examined by ICP-AES. Umbilical cords were removed after birth. Mothers' ages ranged from 26 to 35 yr. It was found that the content of sulfur was the highest ...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02783875
更新日期：1999-09-01 00:00:00
abstract：:The catalytic activity of cerium oxide nanoparticles (CeO2NPs) is responsible for its application as an antitumor agent. This activity may be due to its ability to switch between III and IV oxidation states thereby conferring pro- and antioxidant properties. This study was designed to assess the hepatoprotective poten...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-019-01696-5
更新日期：2020-01-01 00:00:00
abstract：:Vanadium, a trace element in human cells and regarded as an essential nutrient, plays an active role in all tissues. It is known that peroxovanadate-nicotinic acid (POV), a complex compound of vanadium, can decrease hyperglycemia; however, its biochemical mechanism remains unclear. The object of the present study is t...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:74:3:237
更新日期：2000-06-01 00:00:00
abstract：:Metallic tin powder injected into Lewis rats caused marked enlargement of draining lymph nodes with prominent hyperplasia of plasma cells. A low level of dietary protein (8%) or deficiency of choline interfered with the cellular proliferation and reduced the size of the nodes by nearly one-third compared to a normal p...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:85:1:71
更新日期：2002-01-01 00:00:00
abstract：:Tibetan sheep have been observed with mineral deficiencies and marginal deficiencies in Qinghai-Tibetan Plateau. Adequate amounts of essential minerals are critical to maximize the productivity and health of livestock. The objectives of this study were to evaluate the effects of 6 months of mineral block supplementati...
journal_title：Biological trace element research
pub_type： 杂志文章,随机对照试验
doi：10.1007/s12011-015-0593-z
更新日期：2016-08-01 00:00:00
abstract：:The primary objective of the present study is analysis of hair trace elements content in children with communication disorder (CD) and autism spectrum disorder (ASD). A total of 99 children from control, CD, and ASD groups (n = 33) were examined. All children were additionally divided into two subgroups according to a...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-016-0878-x
更新日期：2017-06-01 00:00:00
abstract：:The knowledge about cesium metabolism and toxicity is sparse. Oral intake of cesium chloride has been widely promoted on the basis of the hypothesis referred to as "high pH cancer therapy", a complimentary alternative medicine method for cancer treatment. However, no properly confirmed tumor regression was reported so...
journal_title：Biological trace element research
pub_type： 杂志文章,评审
doi：10.1007/s12011-009-8486-7
更新日期：2010-06-01 00:00:00
abstract：:This study was initiated to examine if variations in the concentrations of cadmium (Cd) and lead (Pb) in water environment may affect metal levels in local foods and body burden of local residents in non-polluted areas in Japan. Two nationwide databases have been made available on concentrations of Cd in locally harve...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-009-8436-4
更新日期：2010-03-01 00:00:00
abstract：:Iron and copper are essential microminerals that are intimately related. The present study was performed to determine the effect of iron-deficiency anemia (IDA) and treatment with iron on laboratory indicators of copper status. Hemoglobin, mean corpuscular volume erythrocyte Zn protoporphyrin, serum ferritin, serum co...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:112:3:213
更新日期：2006-09-01 00:00:00
abstract：:The relaxation rates (1/T(1) and 1/T(2)) in cysts have already been analyzed in terms of materials such as albumin, cholesterol, manganese, iron, and copper. However, the relaxivities of these materials have not been determined yet. In this work, five sets containing the ions, albumin, and cholesterol were prepared by...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-008-8170-3
更新日期：2008-12-01 00:00:00
abstract：:The aim of the current study was to assess relationships between multiple metals burden in human seminal plasma and semen quality parameters. Levels of five metals (lead, manganese, copper, arsenic, and selenium) in human seminal plasma were determined by inductively coupled plasma mass spectrometry (ICP-MS), and the ...
journal_title：Biological trace element research
pub_type： 临床试验,杂志文章
doi：10.1007/s12011-012-9335-7
更新日期：2012-07-01 00:00:00
abstract：:Recently, considerable attention has been paid to the negative effects caused by the presence and constant increase in concentration of heavy metals in the environment, as well as to the determination of their content in human biological samples. In this paper, the concentration of chromium in samples of blood and int...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-018-1328-8
更新日期：2018-12-01 00:00:00
abstract：:The current study investigated the role of selenium (Se) nanoparticles on the growth performance, hemato-biochemical indices, antioxidative, and immune-related genes of European seabass (Dicentrarchus labrax). Therefore, fish with initial weight of 20.53 ± 0.10 g/fish were fed diets with 0, 0.25, 0.5, and 1 mg Se nano...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-020-02431-1
更新日期：2020-10-15 00:00:00
abstract：:The objective of this study was to determine the effects of supplemental chromium as chromium picolinate (CrPic) on productive performance, chromium (Cr) concentration, serum parameters, and colostrum composition in sows. Thirty Yorkshire sows were bred with semen from a pool of Landrace boars. The sows were equally g...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-013-9699-3
更新日期：2013-07-01 00:00:00
abstract：:The aim of this study was to examine the effects of cadmium in concentrations relevant to those detected in human serum on cyclooxygenase-1 (COX-1) and cyclooxygenase-2 (COX-2) expression at mRNA, protein, and enzyme activity levels in THP-1 macrophages. Macrophages were incubated with various cadmium chloride (CdCl2)...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-015-0234-6
更新日期：2015-06-01 00:00:00
abstract：:Three kinds of plant leaves, Chinese white aspen, arborvitae, and pine needles, have been sampled from the Yanshan Oil refinery complex, the Capital Iron and Steel Factory, and Badachu, a control area in Beijing, as biomonitors for air-pollution studies. Each sample was divided into two parts, washed, and unwashed. Th...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02784212
更新日期：1999-01-01 00:00:00
abstract：:Fluoride can induce neurotoxicity, but the mechanism is not clear. In this study, we explored the role of autophagy in F--induced neurotoxicity of Wistar rats. Eighty Wistar rats were randomly divided into four groups: the control group (distilled water containing less than 0.1 mg/L F-) and three NaF-treated groups (F...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-019-01735-1
更新日期：2020-02-01 00:00:00
abstract：:Soaking of a rat diet, high in both plant phytate and phytase, progressively degraded the phytate content with time of soaking. This dephytinization in turn enhanced the digestion of feed organic matter in the animals, and it significantly improved the absorption and retention of minerals and trace elements as observe...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/BF02783810
更新日期：1993-10-01 00:00:00
abstract：:Pituitary dwarfism (hGHD) is known to be associated with trace element deficiency, which causes improper functioning of the involved endocrine system. Previously, we reported on the head hair concentrations of zinc, copper, manganese, and iron from a total of 418 normal subjects (154 male and 264 female). In this repo...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1385/BTER:85:2:127
更新日期：2002-02-01 00:00:00
abstract：:Tomato quality and its potential health benefits are directly related to its chemical composition. The characterization of nutritional properties of Solanum germplasm is essential to choose suitable donor parents for breeding programs. In this sense, wild species could be very useful for tomato fruit quality genetic i...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-010-8738-6
更新日期：2011-06-01 00:00:00
abstract：:In this study, the amount of reduced glutathione (GSH), oxidized glutathione (GSSG), and malondialdehyde (MDA) were determined by high performance liquid chromatography (HPLC), and selenium was determined by using the fluorescence spectrophotometer in eight different species of edible mushrooms. Brittlegill mushroom (...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-016-0715-2
更新日期：2016-12-01 00:00:00
abstract：:This study was aimed to assess the protective effect of sodium selenite on the ileum mucosal immunologic injury induced by AFB1. One hundred eighty-one-day-old healthy male Avian broilers were divided into four groups of three replicates and 15 birds per replicate and fed with basal diet (control group), 0.3 mg/kg AFB...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-0035-3
更新日期：2014-07-01 00:00:00
abstract：:The present study was aimed at estimating the current (2012) dietary intake of arsenic (As), cadmium (Cd), mercury (Hg), and lead (Pb) by the population of Catalonia, Spain. The temporal trends with respect to previous surveys, performed in 2000, 2005, and 2008, were also determined. For that purpose, metal concentrat...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-014-0138-x
更新日期：2014-12-01 00:00:00
abstract：:Guizhou black goats are essential to the production system in the Wumeng prairie in the Western China. This study aimed to determine the influence of nano-copper on antioxidant system in copper-deprived Guizhou black goats. We analyzed mineral contents in soil, forage, and goats' tissues. Blood parameters were also de...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-020-02342-1
更新日期：2020-08-18 00:00:00
abstract：:Previous studies showed the attenuation of both morphine-dependence and morphine-place preference by zinc. Conditioned place preference and aversion are experimental models frequently used to test the reward-stimulating, respectively the aversive effects induced by different stimuli or substances. Addictive substances...
journal_title：Biological trace element research
pub_type： 杂志文章
doi：10.1007/s12011-017-0995-1
更新日期：2017-11-01 00:00:00
