Influenza A and B virus IgG and IgM serology by enzyme immunoassays.

Abstract:

:Enzyme immunoassays (EIA) for IgG and IgM antibodies against influenza A and B virus are described. One hundred and seven subjects with a clinical diagnosis of acute respiratory infection (influenza, bronchitis or pneumonia) were selected for this study during two epidemics of influenza A which occurred in Finland in 1983 and 1985. Paired sera and nasopharyngeal secretions were obtained from all subjects. The sera were tested for influenza A and B antibodies by IgG and IgM EIAs and by complement fixation tests. The nasopharyngeal secretions were tested by an indirect EIA for influenza A and B antigens. The IgG EIA was found to be better than complement fixation for the diagnosis of influenza A infections: only 22% of the significant increases detected by this test were also positive by complement fixation. The additional contribution of the IgM EIA to the number of positives was minimal. It was also found that testing a single 1/1000 dilution of serum for influenza A and 1/100 dilution for influenza B in the IgG EIA gave as many positives as the conventional method of testing several dilutions.

journal_name

Epidemiol Infect

authors

Koskinen P,Vuorinen T,Meurman O

doi

10.1017/s0950268800066863

subject

Has Abstract

pub_date

1987-08-01 00:00:00

pages

55-64

issue

1

eissn

0950-2688

issn

1469-4409

journal_volume

99

pub_type

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