Abstract：
OBJECTIVES:To determine if SAHA, a histone deacetylase inhibitor, decreases ovarian cancer cell viability when combined with paclitaxel in vitro, and to explore molecular alterations of combined paclitaxel+SAHA treatment. METHODS:SKOV3 and Hey ovarian cancer cell lines were treated for 24 h with paclitaxel, then re-treated with SAHA or paclitaxel for an additional 48 h. Protein extracts were prepared at 48 h for western blot analysis. Cell viability was assessed at 72 h using the ApoAlert Annexin V Apoptosis Kit. RESULTS:SAHA causes G1 and G2 cell cycle arrest in ovarian cancer cell lines. Cell viability was significantly reduced by combined paclitaxel+SAHA treatment. In Hey cells, viability was reduced to 67% with paclitaxel, and to 48% with paclitaxel+SAHA (p<0.001). In the SKOV3 cell line, viability was reduced to 70% with continuous paclitaxel treatment, and was further reduced to 57% in the combined treatment group (p<0.05). Increased PARP cleavage was noted in the paclitaxel+SAHA groups. SAHA increased expression of p21cip1/waf1 and p27Kip1, down regulated cyclins A and B, and suppressed CDK1. Paclitaxel induced expression of survivin, an inhibitor of apoptosis protein, was reduced to baseline control levels with the addition of SAHA. The pro-apoptotic protein, Bad, was also increased with SAHA. CONCLUSIONS:Paclitaxel+SAHA reduces cell viability in excess of either agent alone in ovarian cancer cell lines. Cell death is mediated via several mechanisms including G1/G2 arrest from CDK1 downregulation, inhibition of paclitaxel-induced survivin accumulation, and from increased Bad expression.
journal_name
Gynecol Oncoljournal_title
Gynecologic oncologyauthors
Dietrich CS 3rd,Greenberg VL,DeSimone CP,Modesitt SC,van Nagell JR,Craven R,Zimmer SGdoi
10.1016/j.ygyno.2009.09.039subject
Has Abstractpub_date
2010-01-01 00:00:00pages
126-30issue
1eissn
0090-8258issn
1095-6859pii
S0090-8258(09)00738-0journal_volume
116pub_type
杂志文章abstract：BACKGROUND:An epigenetic approach to explaining endometrial carcinogenesis necessitates good understanding of Ras association domain family 1 isoform A (RASSF1A) promoter methylation data from primary studies. AIMS:Differential magnitude of reported associations between RASSF1A promoter methylation and endometrial can...
journal_title：Gynecologic oncology
pub_type： 杂志文章,meta分析,评审
doi：10.1016/j.ygyno.2017.06.017
更新日期：2017-09-01 00:00:00
abstract：:Adenocarcinoma of the urethra is a rare neoplasm which is believed to arise from the periurethral ducts. Four such cases have been diagnosed at our institution during the past 25 years and their clinical courses are summarized in this report. In one patient, a disseminated coagulopathy with a predominantly fibrinolyti...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(85)90034-4
更新日期：1985-10-01 00:00:00
abstract：OBJECTIVE:To evaluate the oncologic and reproductive outcomes and to analyze prognostic factors after fertility-sparing surgery in patients with early and advanced malignant ovarian germ cell tumors (MOGCTs). METHODS:This study included 171 patients who underwent fertility-sparing surgery. Data were gathered from pati...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2017.03.023
更新日期：2017-06-01 00:00:00
abstract：BACKGROUND:Although overall cervical cancer incidence rates have decreased in both black and white women in the U.S. since the mid 1950s due to widespread screening, rates continue to be higher among blacks than among whites. However, whether this pattern differs by age is unknown. METHODS:Cervical cancer cases (1975-...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2012.08.021
更新日期：2012-12-01 00:00:00
abstract：:While many investigators have described the biochemical and physiological similarities between tumor cells and trophoblast cells, in this discourse we will compare primarily their leucocytes, which constitute a large portion of the tumor and its microenvironment as well as the placenta and its microenvironment. There ...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2017.04.019
更新日期：2017-06-01 00:00:00
abstract：OBJECTIVES:Abnormalities in the control of cell proliferation and apoptosis have been suggested to contribute to the development and progression of neoplasia. There are at least two pathways that activate apoptosis. The first is a mitochondria-dependent route governed by bcl-2 family proteins. The second is a parallel ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/s0090-8258(03)00411-6
更新日期：2003-11-01 00:00:00
abstract：OBJECTIVE:Chemotherapy-induced peripheral neuropathy (CIPN) presents itself as sensory peripheral neuropathy (SPN) or motor peripheral neuropathy (MPN). Our aim was to examine the course of SPN and MPN, and their impact on health-related quality of life (HRQoL) among ovarian cancer patients. METHODS:All newly diagnose...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.ygyno.2018.03.052
更新日期：2018-06-01 00:00:00
abstract：:Twelve patients with recurrent squamous cell carcinoma of the cervix restricted to the pelvis were treated with intra-arterial infusion of carboplatin. The initial carboplatin dose was 300 mg/m2, every 4 weeks, and the dose was escalated to 450 mg/m2. Myelosuppression was the dose-limiting toxicity at the 450 mg/m2 do...
journal_title：Gynecologic oncology
pub_type： 临床试验,杂志文章
doi：10.1006/gyno.1994.1136
更新日期：1994-06-01 00:00:00
abstract：OBJECTIVE:Preoperative differentiation of primary and metastatic ovarian tumors is difficult. Young age of the patient, bilateralism and reduced multilocularity are cited characteristics of secondary ovarian malignancies. We sought to identity pre- and perioperative factors which may aid in differentiating metastatic o...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2005.09.046
更新日期：2006-04-01 00:00:00
abstract：:A model system of exfoliated normal human cervicovaginal squamous cells, exfoliated rodent tumor cells, and acellular, viscous, mucuslike material was used to investigate cell deposition on smear preparations made with three different instruments: plastic spatulas, wooden spatulas, and brush-tipped collectors. The tot...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(90)90426-l
更新日期：1990-11-01 00:00:00
abstract：BACKGROUND:The female genital lymphomas are an extremely rare disease accounting for less than 0.5% of gynecological cancers and for 1.5% of all NHL. Because of their low incidence there is no widely accepted consensus on its treatment. Literature data support a radiotherapy-based treatment in case of primary genital l...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2006.08.024
更新日期：2007-02-01 00:00:00
abstract：OBJECTIVES:Previous studies have identified loss of chromosomal regions 7p12-q11.2 and 8p12-p21 in choriocarcinoma suggesting that suppressor genes involved in tumour development may be located within these regions. Our objectives were to refine the regions of loss and evaluate these deletions as prognostic indicators ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2006.04.015
更新日期：2006-11-01 00:00:00
abstract：:Five cases of primary vaginal melanoma were treated at UCLA Medical Center between 1976 and 1986. Two additional cases of melanoma arising at the junction of the vulva and vagina are presented. One of seven (13%) patients is alive, with a median time to recurrence of 7 months, and median survival of 31 months. Four of...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/0090-8258(89)90603-3
更新日期：1989-04-01 00:00:00
abstract：:This report presents 13 patients with epithelial ovarian neoplasms of low malignant potential. Ten patients had serous tumors, 1 had a mucinous tumor, and 2 had endometrioid tumors. Two had Stage I disease, 4 had Stage II disease, and 7 had Stage III disease. All were treated with melphalan. Second-look laparotomy was...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(85)90250-1
更新日期：1985-06-01 00:00:00
abstract：OBJECTIVE:Proliferative activity (PA) may be an indicator of a neoplasm's malignant potential, and it has been described as a prognostic factor in different malignant tumors. It was our aim to study the prognostic significance of PA defined by Ki-S5 and Ki-S2 immunohistochemical staining in a large homogeneously treate...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2006.01.037
更新日期：2006-10-01 00:00:00
abstract：OBJECTIVES:To evaluate reasons for discontinuing intraperitoneal (IP) chemotherapy, and to compare characteristics of patients who did versus did not successfully complete six cycles of IP chemotherapy. METHODS:In a phase III trial, women with optimal stage III ovarian or peritoneal carcinoma were randomly allocated t...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究,随机对照试验
doi：10.1016/j.ygyno.2005.11.013
更新日期：2006-01-01 00:00:00
abstract：OBJECTIVE:Human leukocyte antigens (HLA) play an important role in presenting virus antigens to immune cells that are responsible for the clearance of virus-infected cells and tumor cells. Herein, we evaluated whether genetic variants of HLA-DP and HLA-DQ are associated with cervical cancer risk. METHODS:We genotyped ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2013.02.017
更新日期：2013-05-01 00:00:00
abstract：OBJECTIVE:Lymph node status in endometrial cancer is a major prognostic factor. Sentinel lymph node (SLN) biopsy using radiocolloid and blue dye labeling has emerged as an alternative to systematic lymphadenectomy. This technique requires a preoperative lymphoscintigraphy. The aim of this study was to evaluate the limi...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2008.11.004
更新日期：2009-02-01 00:00:00
abstract：BACKGROUND:Luteinized thecoma of the ovary associated with sclerosing peritonitis is a rare tumor that has no standard definitive treatment regimen. CASE:A 25 year-old patient diagnosed with luteinized thecoma and sclerosing peritonitis in the omentum. The patient received high dose corticosteroids (IV Hydrocortisone ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2008.04.014
更新日期：2008-12-01 00:00:00
abstract：PURPOSE:Intraperitoneal (IP) therapy improves survival compared to intravenous (IV) treatment for women with newly diagnosed, optimally cytoreduced, ovarian cancer. However, the role of IP therapy in recurrent disease is unknown. Preclinical data demonstrated IP administration of the proteasome inhibitor, bortezomib pr...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.ygyno.2017.03.013
更新日期：2017-05-01 00:00:00
abstract：OBJECTIVE:To evaluate the feasibility of an electronic symptom-tracking platform for patients recovering from ambulatory surgery. METHOD:We assessed user response to an electronic system designed to self-report symptoms. Endpoints included compliance, postoperative symptoms, patient satisfaction. An 8-item symptom inv...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2020.07.004
更新日期：2020-10-01 00:00:00
abstract：:Uterine papillary serous carcinoma (UPSC) is an uncommon but aggressive type of endometrial cancer associated with rapid progression of disease and poor prognosis. We investigated 23 cases of UPSC. p53 expression was studied in archival paraffin-embedded tissue by immunohistochemistry. Eleven tumors (47.8%) showed p53...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1998.5113
更新日期：1998-10-01 00:00:00
abstract：OBJECTIVE:Doxorubicin has reported activity in advanced and recurrent cervical cancer but hematologic toxicity has limited its use in some combinations. To determine the level of activity and potential for use in future combinations, a phase II trial of pegylated liposomal doxorubicin as second-line therapy in advanced...
journal_title：Gynecologic oncology
pub_type： 杂志文章,多中心研究
doi：10.1016/j.ygyno.2005.11.048
更新日期：2006-08-01 00:00:00
abstract：OBJECTIVES:The aim of the current study is to investigate the clinical and molecular factors associated with cytoreduction among women with advanced stage epithelial ovarian carcinoma EOC. METHODS:Seventy-two women with FIGO stage III and IV EOC or primary peritoneal carcinoma (PPC) underwent similar attempt at surgic...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2004.07.045
更新日期：2004-11-01 00:00:00
abstract：:Stage IIB-IV squamous cell carcinoma of the cervix when treated by irradiation has a significant failure rate. Causes of pelvic and distant failure are discussed. New techniques employed to improve local control and decrease distant metastasis are presented. Data on morbidity, mortality, and survival will be reviewed ...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/0090-8258(86)90135-6
更新日期：1986-03-01 00:00:00
abstract：BACKGROUND:Although renal cell carcinoma (RCC) is characterized with unpredictable clinical presentation, multiple genital tract metastases are still surprising and mode of spread is obscure. CASE:We report a case of RCC metastases to uterine cervix and vagina 1 year after radical nephrectomy in a 19-year-old virgin. ...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2005.06.004
更新日期：2005-10-01 00:00:00
abstract：OBJECTIVE:In the last decade several studies have been published using proteomics to unravel molecular pathways and to find biomarkers which can be used for diagnosis and/or prognostication in ovarian cancer. This review gives an overview of proteomic studies performed in ovarian cancer focusing on the nature of sample...
journal_title：Gynecologic oncology
pub_type： 杂志文章,评审
doi：10.1016/j.ygyno.2009.09.005
更新日期：2009-12-01 00:00:00
abstract：OBJECTIVES:Early detection of ovarian cancer should improve overall survival. Multiple serum markers have been evaluated as possible tests to detect early stage disease, but few urine markers have been studied. Mesothelin has been detected in serum from patients with ovarian cancer, but has not been previously reported...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2007.04.022
更新日期：2007-09-01 00:00:00
abstract：OBJECTIVE:To analyze the expression of Heparanase, an enzyme involved in cancer metastasis and angiogenesis, in ovarian and breast carcinoma cells in effusions. METHODS:Heparanase protein expression was analyzed in malignant effusions from ovarian (=200) and breast (=41) carcinoma patients using immunocytochemistry. T...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1016/j.ygyno.2006.08.045
更新日期：2007-02-01 00:00:00
abstract：:Human papillomavirus (HPV) containing cell lines derived from human cervical intraepithelial neoplasia (CIN) can offer valuable insights into the role of HPV in cervical neoplasia and can help in the understanding of the cellular changes that fuel the progression toward malignancy. We describe the growth and different...
journal_title：Gynecologic oncology
pub_type： 杂志文章
doi：10.1006/gyno.1993.1131
更新日期：1993-06-01 00:00:00
