Abstract:
:The role of JNK in neutrophil chemotaxis and killing of microbial pathogens remains unclear. Using a recently described cell-permeable peptide inhibitor of the JNK pathway, based on the JBD of JIP-1, coupled to the protein transduction domain of HIV-TAT (TAT-JIP), in association with control peptides, we demonstrate that the JNK pathway plays a major role in regulating human neutrophil chemotaxis and killing of microbial pathogens. Serum-opsonized Staphylococcus aureus elicited JNK activation and c-jun phosphorylation. The activation of the JNK pathway and bactericidal activity were inhibited by the TAT-JIP peptide. The stimulation of oxygen radical generation by S. aureus was dependent on the JNK signaling pathway, as was the phagocytosis of serum-opsonized bacteria. Chemotaxis to activated serum complement but not random migration was inhibited by the TAT-JIP peptide. The findings demonstrate a major role for the JNK signaling pathway in neutrophil-mediated defense against microbial pathogens.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Yeh MC,Mukaro V,Hii CS,Ferrante Adoi
10.1189/jlb.0609399subject
Has Abstractpub_date
2010-05-01 00:00:00pages
925-32issue
5eissn
0741-5400issn
1938-3673pii
jlb.0609399journal_volume
87pub_type
杂志文章abstract::Chemokines play a crucial role in combating microbial infection by recruiting blood neutrophils to infected tissue. In mice, the chemokines Cxcl1/KC and Cxcl2/MIP2 fulfill this role. Cxcl1 and Cxcl2 exist as monomers and dimers, and exert their function by activating the Cxcr2 receptor and binding glycosaminoglycans (...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.3A0820-207R
更新日期:2020-09-02 00:00:00
abstract::Our previous studies demonstrated that i.p. injection of late apoptotic P388 cells caused phagocytosis by macrophages and transient infiltration of neutrophils into the peritoneal cavity. As neutrophils are known to function as effectors as well as regulators in the immune response, we examined the roles of infiltrati...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0606399
更新日期:2007-02-01 00:00:00
abstract::We identified fresh human leukocytes as an abundant source of the candidate epithelial tumor suppressor gene, Ecrg4, an epigenetically regulated gene, which unlike other tumor suppressor genes, encodes an orphan-secreted, ligand-like protein. In human cell lines, Ecrg4 gene expression was low, Ecrg4 protein undetectab...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1011503
更新日期:2012-05-01 00:00:00
abstract::Antagonism of ROS signaling can inhibit cell apoptosis and autophagy, thus favoring the maintenance and expansion of hematopoietic stem cells. Alpha lipoic acid (ALA), a small antioxidant molecule, affects cell apoptosis by lowering the ROS level. In this study, we show that ALA promoted production of human pluripoten...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.1A0520-179R
更新日期:2020-12-01 00:00:00
abstract::Extracellular ATP mediates a diverse array of biological responses in many cell types and tissues, including immune cells. We have demonstrated that ATP induces purinergic receptor P2X(7) mediated membrane permeabilization, apoptosis, and cytokine expression in murine mast cells (MCs). Here, we report that MCs deficie...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,收录出版
doi:10.1189/jlb.0808470
更新日期:2009-04-01 00:00:00
abstract::It has been reported recently that granulocyte-colony stimulating factor (G-CSF) is degraded upon exposure to human neutrophil elastase (HNE), and this has a negative effect on the ability of the cytokine to promote the in vitro proliferation and maturation of CD34+ cells. This has important implications on the possib...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0803378
更新日期:2004-03-01 00:00:00
abstract::Besides their growth-inhibiting and differentiation-inducing properties, retinoids have been shown to exert immunomodulatory and anti-inflammatory functions by mechanisms that are not well understood. Tumor necrosis factor-alpha (TNF), a cytokine produced by mononuclear phagocytes, has been shown to be an important me...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.55.3.336
更新日期:1994-03-01 00:00:00
abstract::Activation of the microvasculature is a major component of the inflammatory response. During inflammation the vascular endothelium not only becomes more permeable to plasma proteins but also develops adhesion molecules that initiate the local immigration of leukocytes. We describe herein the in vivo changes in the thr...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.60.6.692
更新日期:1996-12-01 00:00:00
abstract::IL-17 has been associated with selected inflammatory and autoimmune diseases. We characterized the expression of this proinflammatory cytokine following HSV-1 corneal infection and investigated whether IL-17R signaling modulated the host response to the viral pathogen at early time-points postinfection. IL-17 was elev...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0807571
更新日期:2008-02-01 00:00:00
abstract::Mutations in the gene JAGN1 were recently discovered in patients with severe congenital neutropenia (SCN). Neutrophils release neutrophil extracellular traps (NETs) consisting of decondensed chromatin decorated with various granular proteins such as neutrophil elastase and myeloperoxidase (MPO) to combat microbial inf...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/JLB.4A0118-030RR
更新日期:2018-12-01 00:00:00
abstract::Although corneal allotransplantation is performed in the immune-privileged cornea, many grafts are still rejected after transplantation. This study examined the role of chemokine receptor D6 expression in a corneal allograft rejection, investigated the modulation of D6 expression in cells, and determined the effect of...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.5A0414-233RR
更新日期:2015-02-01 00:00:00
abstract::Neutrophil activation is a multistep process. In vitro activation of neutrophils with semiphysiological activators is optimal only after preactivation or priming with cytokines, chemotaxins, and/or bacterial products. Until now, no antibodies have been developed that can distinguish between resting and (cytokine) prim...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::Rac2 is a hematopoietic-specific Rho-GTPase that plays a stimulus-specific role in regulating reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation and other functional responses in neutrophils. In this study, rac2-/- neutrophils were shown to have significantly decreased NADPH oxidase activit...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0705371
更新日期:2006-01-01 00:00:00
abstract::Compared with CD4+25+ regulatory T cells (Tregs), the mechanisms for natural, polyclonal CD8+25+ Treg immune suppression have been significantly less studied. We previously showed that polyclonal T cells can acquire antigen-specific targeting activity through arming with exosomal peptide-MHC (pMHC). In this study, we ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.3A0716-295RR
更新日期:2017-05-01 00:00:00
abstract::Eosinophils are generally linked to innate host defense against helminths, as well as the pathologies associated with allergic diseases, such as asthma. Nonetheless, the activities of eosinophils remain poorly understood, which in turn, has prevented detailed definitions of their role(s) in health and disease. Homolog...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0213089
更新日期:2013-07-01 00:00:00
abstract::The ability of neutrophils to sense and migrate toward damaged tissue is a vital component of the innate immune response. Paradoxically, this same migration serves as the hallmark of a number of inflammatory conditions, including ischemic reperfusion injury, atherosclerosis, arthritis, and Crohn's disease. More recent...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0608382
更新日期:2009-01-01 00:00:00
abstract::Previously, we described a novel glycosylphosphatidyl inositol (GPI)-anchored glycoprotein (designated GPI-80) on human neutrophils and monocytes that may regulate beta(2) integrin-dependent neutrophil adherence and migration. However, the mechanism regulating beta(2) integrin remains to be clarified. To study this, w...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::IL-18 has a potential to up-regulate the Th1 and Th2 immune responses. It is known that IL-18, in synergy with IL-12, augments the Th1 response to bacterial infections, but it also augments the Th2 response to allergic disorders in the absence of IL-12. Although the Th1 and Th2 immune responses cross-regulate each oth...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.1012502
更新日期:2013-05-01 00:00:00
abstract::Lipopolysaccharide-induced CXC chemokine (LIX) is a novel murine neutrophil-chemoattractant CXC chemokine cloned as a glucocorticoid-attenuated response gene. We investigated LIX message expression in an acute endotoxemia model. LIX message peaks later than KC or macrophage inflammatory protein-2 (MIP-2) and remains e...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.64.4.494
更新日期:1998-10-01 00:00:00
abstract::Upon exposure to immune or inflammatory stimuli, dendritic cells (DC) migrate from peripheral tissues to lymphoid organs, where they present antigen. The molecular basis for the peculiar trafficking properties of DC is largely unknown. In this study, mouse DC were generated from CD34+ bone marrow precursors and cultur...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.66.3.489
更新日期:1999-09-01 00:00:00
abstract::Cytokines and chemokines play an essential role in recruiting leukocytes from the circulation to the peripheral sites of inflammation by modulating cellular interactions with endothelial cell ligands and extracellular matrix (ECM). Herein, we examined regulation of T cell adhesion to ECM ligands by two major proinflam...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::Polyclonal B cell activation is not a peculiar characteristic to a particular infection, as many viruses, bacteria, and parasites induce a strong polyclonal B cell response resulting in hyper-gamma-globulinemia. Here, we discuss the different roles proposed for polyclonal B cell activation, which can be crucial for ea...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.0407214
更新日期:2007-11-01 00:00:00
abstract::A previously unsuspected, considerable proportion of newly synthesized polypeptides are hydrolyzed rapidly by proteasomes, possibly competing with endogenous substrates and altering proteostasis. In view of the anti-cancer effects of PIs, we set out to achieve a quantitative assessment of proteasome workload in cells ...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1011497
更新日期:2012-11-01 00:00:00
abstract::Glucosamine, an amino monosaccharide naturally occurring in the connective and cartilage tissues, contributes to maintaining the strength, flexibility, and elasticity of these tissues. In recent years, glucosamine has been used widely to treat osteoarthritis in humans and animal models. Neutrophils, which usually func...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-04-01 00:00:00
abstract::The neutrophil inflammatory response can be altered profoundly by contact with extracellular matrix proteins (ECMs). We characterized functional responses (intracellular calcium, actin polymerization, degranulation, adhesion, and oxidative burst) of bovine neutrophils adhered to selected ECM proteins [collagen IV, lam...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
更新日期:2002-05-01 00:00:00
abstract::The human cathelicidin LL-37 is a cationic host defense peptide (antimicrobial peptide) expressed primarily by neutrophils and epithelial cells. This peptide, up-regulated under conditions of inflammation, has immunomodulatory and antimicrobial functions. We demonstrate that LL-37 is a potent inhibitor of human neutro...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.1005560
更新日期:2006-09-01 00:00:00
abstract::Interferons (IFN) and lipopolysaccharide (LPS) cause multiple changes in isoprenoid-modified proteins in murine macrophages, the most dramatic being the expression of a prenyl protein of 65 kDa. The guanylate binding proteins (GBPs) are IFN-inducible GTP-binding proteins of approximately 65 kDa that possess a CaaX mot...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1002/jlb.60.3.423
更新日期:1996-09-01 00:00:00
abstract::Cathepsins (Cat) in antigen presenting cells (APC) control antigen processing as well as major histocompatibility complex class II transport and function. The set of active Cat and the subcellular architecture of the class II antigen presentation compartment are largely unknown in primary human APC, including peripher...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0802398
更新日期:2003-02-01 00:00:00
abstract::Thimerosal is an organic mercury compound that is used as a preservative in vaccines and pharmaceutical products. Recent studies have shown a TH2-skewing effect of mercury, although the underlying mechanisms have not been identified. In this study, we investigated whether thimerosal can exercise a TH2-promoting effect...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:10.1189/jlb.0706467
更新日期:2007-02-01 00:00:00
abstract::Cyclophilin A (CypA), a peptidylprolyl cis-trans isomerase, is a ubiquitous and multifunctional protein. In addition to its role as a host-cell receptor for cyclosporine A, CypA has diverse functions in inflammatory conditions and diseases. CypA secreted in response to inflammatory stimuli binds to the cell surface vi...
journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
doi:10.1189/jlb.3RU0317-090RR
更新日期:2017-10-01 00:00:00