Abstract:
:Eosinophils are generally linked to innate host defense against helminths, as well as the pathologies associated with allergic diseases, such as asthma. Nonetheless, the activities of eosinophils remain poorly understood, which in turn, has prevented detailed definitions of their role(s) in health and disease. Homologous recombination in embryonic stem cells was used to insert a mammalianized Cre recombinase in the ORF encoding Epx. This knock-in strategy overcame previous inefficiencies associated with eosinophil-specific transgenic approaches and led to the development of a knock-in strain of mice (eoCRE), capable of mediating recombination of "floxed" reporter cassettes in >95% of peripheral blood eosinophils. We also showed that this Cre expression was limited exclusively to eosinophil-lineage committed cells with no evidence of Cre-mediated toxicity. The efficiency and specificity of Cre expression in eoCRE mice were demonstrated further in a cross with a knock-in mouse containing a "(flox-stop-flox)" DTA cassette at the ROSA26 locus, generating yet another novel, eosinophil-less strain of mice. The development of eoCRE mice represents a milestone in studies of eosinophil biology, permitting eosinophil-specific gene targeting and overexpression in the mouse as part of next-generation studies attempting to define eosinophil effector functions.
journal_name
J Leukoc Bioljournal_title
Journal of leukocyte biologyauthors
Doyle AD,Jacobsen EA,Ochkur SI,Willetts L,Shim K,Neely J,Kloeber J,Lesuer WE,Pero RS,Lacy P,Moqbel R,Lee NA,Lee JJdoi
10.1189/jlb.0213089subject
Has Abstractpub_date
2013-07-01 00:00:00pages
17-24issue
1eissn
0741-5400issn
1938-3673pii
jlb.0213089journal_volume
94pub_type
杂志文章abstract::Evidence that gamma/delta T cells play a broad, immunoregulatory role has been accumulating steadily. We show here that myeloid cells are disregulated after peritoneal infection with Listeria monocytogenes in mice lacking gamma/delta T cells. Inflammatory populations of neutrophils and monocytes recruited to the site ...
journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章,评审
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
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journal_title:Journal of leukocyte biology
pub_type: 杂志文章
doi:
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abstract::The expression of alkaline phosphatase (APase) activity by purified B cells from lipopolysaccharide (LPS)-hyporesponsive C3H/HeJ mice was determined. Optimal APase activity was expressed after costimulation with interleukin-5 and dextran sulfate (DXS), whereas LPS, which is highly effective on B lymphocytes from norma...
journal_title:Journal of leukocyte biology
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journal_title:Journal of leukocyte biology
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