Abstract:
:Here we report on the development of a novel neuroelectronic interface consisting of an array of noninvasive gold-mushroom-shaped microelectrodes (gMmicroEs) that practically provide intracellular recordings and stimulation of many individual neurons, while the electrodes maintain an extracellular position. The development of this interface allows simultaneous, multisite, long-term recordings of action potentials and subthreshold potentials with matching quality and signal-to-noise ratio of conventional intracellular sharp glass microelectrodes or patch electrodes. We refer to the novel approach as "in-cell recording and stimulation by extracellular electrodes" to differentiate it from the classical intracellular recording and stimulation methods. This novel technique is expected to revolutionize the analysis of neuronal networks in relations to learning, information storage and can be used to develop novel drugs as well as high fidelity neural prosthetics and brain-machine systems.
journal_name
J Neurophysioljournal_title
Journal of neurophysiologyauthors
Hai A,Shappir J,Spira MEdoi
10.1152/jn.00265.2010subject
Has Abstractpub_date
2010-07-01 00:00:00pages
559-68issue
1eissn
0022-3077issn
1522-1598pii
jn.00265.2010journal_volume
104pub_type
杂志文章abstract::Horseradish peroxidase- (HRP) filled microelectrodes have been used to examine the anatomy and physiology of "commissural interneurons," a morphologically defined class of spinal cord interneuron in Xenopus laevis embryos. Commissural interneurons have unipolar cell bodies in the dorsal half of the spinal cord. Their ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.51.6.1257
更新日期:1984-06-01 00:00:00
abstract::Metabotropic receptors modulate numerous cellular processes by intracellular Ca2+ signaling, but less is known about their role in regulating mitochondrial metabolic function within the CNS. In this study, we demonstrate in area CA3 of rat organotypic hippocampal slice cultures that glutamatergic, serotonergic, and mu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00042.2003
更新日期:2003-08-01 00:00:00
abstract::Foot drop is very common among people with chronic incomplete spinal cord injury (SCI) and likely stems from SCI that disturbs the corticospinal activation of the ankle dorsiflexor tibialis anterior (TA). Thus, if one can recover or increase the corticospinal excitability reduced by SCI, motor function recovery may be...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00557.2018
更新日期:2019-03-01 00:00:00
abstract::Many laboratories have reported the successful regeneration of neurons across damaged portions of the spinal cord. Associated improvements in hindlimb locomotor movements have been attributed to the formation of functional neuronal connections with the locomotor central pattern generator (CPG). However, regenerating a...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00814.2006
更新日期:2007-03-01 00:00:00
abstract::Paw-shake responses (PSRs) were evoked by wrapping masking tape around the hind-paw in nine cats spinalized at the T12 level either at 2 or 12 wk of age or as young adults (10-12 mo). Electromyographic responses of ankle extensors (LG and SOL) and one ankle flexor (TA) were recorded through the 6th mo after cord trans...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1984.51.4.680
更新日期:1984-04-01 00:00:00
abstract::Particular types of amacrine cells of the vertebrate retina show oscillatory membrane potentials (OMPs) in response to light stimulation. Historically it has been thought the oscillations arose as a result of circuit properties. In a previous study we found that in some amacrine cells, the ability to oscillate was an ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00092.2003
更新日期:2003-07-01 00:00:00
abstract::Stimulus-specific adaptation is the phenomenon whereby neural response magnitude decreases with repeated stimulation. Inconsistencies between recent nonhuman animal recordings and computational modeling suggest dynamic influences on stimulus-specific adaptation. The present human electroencephalography (EEG) study inv...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00634.2014
更新日期:2015-04-01 00:00:00
abstract::The respective roles of ventral and dorsal cortical processing streams are still under discussion in both vision and audition. We characterized neural responses in the caudal auditory belt cortex, an early dorsal stream region of the macaque. We found fast neural responses with elevated temporal precision as well as n...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00436.2013
更新日期:2014-04-01 00:00:00
abstract::Recent evidence has suggested that theta-frequency (4-7 Hz) oscillations around the human anterior cingulate cortex (ACC) and frontal cortex--that is, frontal midline theta (Fm theta) oscillations--may be involved in attentional processes in the brain. However, little is known about the physiological basis of Fm theta...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00730.2005
更新日期:2006-05-01 00:00:00
abstract::Responses have been recorded from primary endings of muscle spindles in the cat soleus muscle. Changes in spindle responsiveness were measured following a period of conditioning that consisted of a series of rapid stretches or of tetanic ventral root stimulation. In the testing procedure the response of a single spind...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1986.56.2.451
更新日期:1986-08-01 00:00:00
abstract::When a movement results in error, the nervous system amends the motor commands that generate the subsequent movement. Here we show that this adaptation depends not just on error, but also on passage of time between the two movements. We observed that subjects learned a reaching task faster, i.e., with fewer trials, wh...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01281.2006
更新日期:2007-06-01 00:00:00
abstract::1. Adaptation and disadaptation rates determine the temporal response properties of sensory receptor cells. In chemoreception, temporal filter properties of receptor cells are poorly understood. We studied the time course of disadaptation in lobster antennular chemoreceptor cells by using in situ high-resolution stimu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.2.1340
更新日期:1996-08-01 00:00:00
abstract::Vision in natural situations is different from the paradigms generally used to study vision in the laboratory. In natural vision, stimuli usually appear in a receptive field as the result of saccadic eye movements rather than suddenly flashing into view. The stimuli themselves are rich with meaningful and recognizable...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00733.2011
更新日期:2012-07-01 00:00:00
abstract::The creation of several prestin knockout and knockin mouse lines has demonstrated the importance of the intrinsic outer hair cell membrane protein prestin to mammalian hearing. However, the structure of prestin remains largely unknown, with even its major features in dispute. Several studies have suggested that presti...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00496.2011
更新日期:2011-11-01 00:00:00
abstract::Neurons in the visual cortex respond selectively to stimulus orientation and spatial frequency. Changes in response amplitudes of these neurons could be the neurophysiological basis of orientation and spatial frequency discrimination. We have estimated the minimum differences in stimulus orientation and spatial freque...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1987.57.3.755
更新日期:1987-03-01 00:00:00
abstract::The intrinsic properties of tonically firing neurons in the cochlear nucleus contribute to representing average sound intensity by favoring synaptic integration across auditory nerve inputs, reducing phase locking to fine temporal acoustic structure and enhancing envelope locking. To determine whether tonically firing...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00092.2012
更新日期:2012-11-01 00:00:00
abstract::Families of peptide neuromodulators are believed to play important roles in neural networks that control behaviors. Here, we investigate the expression and role of one such group of modulators, the myomodulins, in the feeding system of Lymnaea stagnalis. Using a combination of in situ hybridization and antibody staini...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1998.79.5.2460
更新日期:1998-05-01 00:00:00
abstract::It is well accepted that pain is a multidimensional experience, but little is known of how the brain represents these dimensions. We used positron emission tomography (PET) to indirectly measure pain-evoked cerebral activity before and after hypnotic suggestions were given to modulate the perceived intensity of a pain...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2001.86.1.402
更新日期:2001-07-01 00:00:00
abstract::The deep dorsal horn represents a major site for the integration of spinal sensory information. The bulbospinal monoamine transmitters, released from serotonergic, noradrenergic, and dopaminergic systems, exert modulatory control over spinal sensory systems as does acetylcholine, an intrinsic spinal cord biogenic amin...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2001.86.5.2183
更新日期:2001-11-01 00:00:00
abstract::When they are 1-2 mo old, domesticated Mongolian gerbils begin having initially mild seizures which become more severe with age. To evaluate the development of this increasing seizure severity, we obtained field potential responses of the dentate gyrus to paired-pulse stimulation of the perforant path during seizures....
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.2002.88.2.783
更新日期:2002-08-01 00:00:00
abstract::This study was prompted by recent evidence for the existence of positive force feedback in feline locomotor control. Our aim was to establish some basic properties of positive force feedback in relation to load compensation, stability, intrinsic muscle properties, and interaction with displacement feedback. In human s...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1997.77.6.3226
更新日期:1997-06-01 00:00:00
abstract::1. In taste neurophysiology, from Pfaffmann's (49, 50) pioneering work until the present, the possibility of types of neurons corresponding in some sense with the "primary" taste qualities of Henning (33) has been entertained: recently types of gustatory neurons in peripheral nerves have been established according to ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1979.42.5.1390
更新日期:1979-09-01 00:00:00
abstract::1. The medullary reticular formation (MRF) is known to be involved in the modulation of certain reproductive behaviors. Ejaculation in the male, disrupted after spinal transection, may depend on a spinal-bulbo-spinal connection. To determine whether single neurons in the MRF receive sensory input from the male genital...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1996.76.4.2474
更新日期:1996-10-01 00:00:00
abstract::Individuals with cerebral palsy (CP) experience impairments in the control of head and neck movements, suggesting dysfunction in brain stem circuitry. To examine if brain stem circuitry is altered in CP, we compared reflexes evoked in the sternocleidomastoid (SCM) muscle by trigeminal nerve stimulation in adults with ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00185.2018
更新日期:2018-09-01 00:00:00
abstract::The human auditory system has an exceptional ability to separate competing sounds, but the neural mechanisms that underlie this ability are not understood. Responses of inferior colliculus (IC) neurons to "mistuned" complex tones were measured to investigate possible neural mechanisms for spectral segregation. A mistu...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.01194.2004
更新日期:2005-11-01 00:00:00
abstract::In free viewing, the eyes return to previously visited locations rather frequently, even though the attentional and memory-related processes controlling eye-movement show a strong antirefixation bias. To overcome this bias, a special refixation triggering mechanism may have to be recruited. We probed the neural eviden...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00121.2018
更新日期:2018-11-01 00:00:00
abstract::Electrical stimulation of the median nerve at the wrist may elicit a blink reflex [hand blink reflex (HBR)] mediated by a neural circuit at brain stem level. As, in a Sherringtonian sense, the blink reflex is a defensive response, in a series of experiments we tested, in healthy volunteers, whether and how the HBR is ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00731.2011
更新日期:2012-02-01 00:00:00
abstract::In this paper, the predictive capability of surface and untargeted intramuscular electromyography (EMG) was compared with respect to wrist-joint torque to quantify which type of measurement better represents joint torque during multiple degrees-of-freedom (DoF) movements for possible application in prosthetic control....
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00086.2013
更新日期:2013-06-01 00:00:00
abstract::Substance P initiates locomotion when injected in the brain stem of mammals. This study examined the possible role of this peptide on the supraspinal locomotor command system in lampreys. Substance P was bath applied or locally injected into an in vitro isolated brain stem, and the effects of the drug were examined on...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.00401.2004
更新日期:2005-04-01 00:00:00
abstract::1. We have investigated the electrical properties of neurons acutely dissociated from the substantia nigra zona compacta (SNZC) of the postnatal rat with whole cell patch-clamp recordings. Retrogradely labeled nigrostriatal neurons were identified with the use of rhodamine-labeled fluorescent latex microspheres. Over ...
journal_title:Journal of neurophysiology
pub_type: 杂志文章
doi:10.1152/jn.1990.64.1.262
更新日期:1990-07-01 00:00:00