Visualizing of the cellular uptake and intracellular trafficking of exosomes by live-cell microscopy.

Abstract:

:Cells release exosomes to transfer various molecules to other cells. Exosomes are involved in a number of physiological and pathological processes. They are emerging great potential utility for diseases diagnosis and treatment recently. However, the internalization and intracellular trafficking of exosomes have not been described clearly. In this work, exosomes were isolated from the culture medium of PC12 cells, labeled by lipophilic dye and amino-reactive fluorophore, incubated with resting PC12 cells. The results of live-cell microscopy indicated that exosomes were internalized through endocytosis pathway, trapped in vesicles, and transported to perinuclear region. Particle tracking fluorescent vesicles suggested that the active transport of exosomes may be mediated by cytoskeleton. The proteins on exosome membrane were found to be released from exosomes and trapped in lysosome. The inverted transport of lipophilic dye from perinuclear region to cell peripheries was revealed, possibly caused by recycling of the exosome lipids. This study provides new sight into the mechanisms of exosome uptake and intracellular fate.

journal_name

J Cell Biochem

authors

Tian T,Wang Y,Wang H,Zhu Z,Xiao Z

doi

10.1002/jcb.22733

subject

Has Abstract

pub_date

2010-10-01 00:00:00

pages

488-96

issue

2

eissn

0730-2312

issn

1097-4644

journal_volume

111

pub_type

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