Protective effect of thymoquinone in experimental testicular torsion.

Abstract:

OBJECTIVES:To investigate the protective role of thymoquinone (TQ) on unilateral testicular ischemia-reperfusion (I/R) injury in mice. MATERIALS AND METHODS:Experiments were performed on male C57BL/6 mice (8 weeks old, 20-25 g). The animals were divided into 3 groups including 6 mice in each group: control (sham), torsion/detorsion (TD) and TD+TQ. Mice, except the sham-operated group, were subjected to left unilateral torsion (720° rotation in the clockwise direction). The experiments were finished after sham operation time for controls, 120 min torsion and 240 min detorsion for the other groups. In the TD+TQ group 10 mg TQ was injected intraperitoneally 30 min before detorsion. RESULTS:In the TD group total oxidative stress (TOS), oxidative stress index (OSI) and malondialdehyde (MDA) levels were higher than in the controls. TQ treatment decreased MDA, TOS and OSI values, but did not affect the total antioxidant capacity and myeloperoxidase activity in the TD+TQ group. Upon histological examination, mice in the TD group displayed moderate-to-severe disruption of the seminiferous epithelium. Treatment with TQ resulted in significantly reduced histological damage associated with I/R injury. CONCLUSION:Our results suggested that TQ treatment may have a protective effect on testicular I/R injury.

journal_name

Urol Int

journal_title

Urologia internationalis

authors

Gökçe A,Oktar S,Koc A,Gonenci R,Yalcinkaya F,Yonden Z,Duru M

doi

10.1159/000318890

subject

Has Abstract

pub_date

2010-01-01 00:00:00

pages

461-5

issue

4

eissn

0042-1138

issn

1423-0399

pii

000318890

journal_volume

85

pub_type

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