Abstract:
:An immunocompetent, permissive, small-animal model would be valuable for the study of human immunodeficiency virus type 1 (HIV-1) pathogenesis and for the testing of drug and vaccine candidates. However, the development of such a model has been hampered by the inability of primary rodent cells to efficiently support several steps of the HIV-1 replication cycle. Although transgenesis of the HIV receptor complex and human cyclin T1 have been beneficial, additional late-phase blocks prevent robust replication of HIV-1 in rodents and limit the range of in vivo applications. In this study, we explored the HIV-1 susceptibility of rabbit primary T cells and macrophages. Envelope-specific and coreceptor-dependent entry of HIV-1 was achieved by expressing human CD4 and CCR5. A block of HIV-1 DNA synthesis, likely mediated by TRIM5, was overcome by limited changes to the HIV-1 gag gene. Unlike with mice and rats, primary cells from rabbits supported the functions of the regulatory viral proteins Tat and Rev, Gag processing, and the release of HIV-1 particles at levels comparable to those in human cells. While HIV-1 produced by rabbit T cells was highly infectious, a macrophage-specific infectivity defect became manifest by a complex pattern of mutations in the viral genome, only part of which were deamination dependent. These results demonstrate a considerable natural HIV-1 permissivity of the rabbit species and suggest that receptor complex transgenesis combined with modifications in gag and possibly vif of HIV-1 to evade species-specific restriction factors might render lagomorphs fully permissive to infection by this pathogenic human lentivirus.
journal_name
J Viroljournal_title
Journal of virologyauthors
Tervo HM,Keppler OTdoi
10.1128/JVI.01607-10subject
Has Abstractpub_date
2010-12-01 00:00:00pages
12300-14issue
23eissn
0022-538Xissn
1098-5514pii
JVI.01607-10journal_volume
84pub_type
杂志文章abstract::Heat shock protein 70 (Hsp70) is incorporated within the membrane of primate lentiviral virions. Here we demonstrate that Hsp70 is also incorporated into oncoretroviral virions and that it remains associated with membrane-stripped human immunodeficiency virus type 1 (HIV-1) virion cores. To determine if Hsp70 promotes...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.79.9.5557-5567.2005
更新日期:2005-05-01 00:00:00
abstract::The development of immunization strategies to protect against ocular infection with herpes simplex virus 1 (HSV-1) must address the issue of the effects of the strategy on the establishment of latency in the trigeminal ganglia (TG). It is the reactivation of this latent virus that can cause recurrent disease and corne...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00913-14
更新日期:2014-07-01 00:00:00
abstract::Understanding the correlates of immune protection against human immunodeficiency virus and simian immunodeficiency virus (SIV) will require defining the entire cellular immune response against the viruses. Here, we define two novel translation products from the SIV env mRNA that are targeted by the T-cell response in ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00138-09
更新日期:2009-10-01 00:00:00
abstract::V2/V3 conformational epitope antibodies that broadly neutralize HIV-1 (PG9 and PG16) have been recently described. Since an elicitation of previously known broadly neutralizing antibodies has proven elusive, the induction of antibodies with such specificity is an important goal for HIV-1 vaccine development. A critica...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.05045-11
更新日期:2011-10-01 00:00:00
abstract::The outer layer of the bacteriophage phi 6 nucleocapsid (NC) was removed by EDTA and reassociated with the core in the presence of Ca2+ or Mg2+. The core was relatively inaccessible to trypsin digestion, was composed of protein P1, and was in the dodecahedral framework reported previously. (H.T. Steely, Jr., and D. La...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.8.2621-2623.1987
更新日期:1987-08-01 00:00:00
abstract::The v-rel oncogene encoded by reticuloendotheliosis virus strain T is the acutely transforming member of the Rel/NF-kappaB family of transcription factors. In v-Rel-transformed cells, v-Rel exists as homodimers or heterodimers with the endogenous Rel/NF-kappaB proteins c-Rel, NF-kappaB1, NF-kappaB2, and RelA. To exami...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/jvi.76.10.4928-4939.2002
更新日期:2002-05-01 00:00:00
abstract::Synthesis of the fiber protein of human adenovirus serotype 2 (Ad2) is 100-fold lower in abortively infected monkey cells, compared with productive infections, despite only a 5- to 10-fold reduction in fiber mRNA levels. Previously Anderson and Klessig (Proc. Natl. Acad Sci. USA 81:4023-4027, 1984) demonstrated a dire...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.10.4376-4385.1989
更新日期:1989-10-01 00:00:00
abstract::To assess whether nitric oxide synthase 2 (NOS2) fulfills the criteria of an innate resistance locus against an acute viral infection, we inoculated genetically deficient NOS2-/- mice with virulent ectromelia virus (EV), the causative agent of mousepox. NOS2-/- mice proved highly susceptible to EV yet showed no diminu...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.72.9.7703-7706.1998
更新日期:1998-09-01 00:00:00
abstract::The RNA-dependent RNA polymerase (NS5B) of hepatitis C virus (HCV) is an unusually attractive target for drug discovery since it contains five distinct drugable sites. The success of novel antiviral therapies will require nonnucleoside inhibitors to be active in at least patients infected with HCV of subtypes 1a and 1...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01980-09
更新日期:2010-03-01 00:00:00
abstract::The genome of hepatitis A virus (HAV) was reverse transcribed into cDNA and molecularly cloned. cDNA clones coding for the capsid protein VP1 that carries the major HAV antigen were cloned into the expression vector pUR290 and expressed in Escherichia coli. The recombinant fusion protein reacted in an immunoblot with ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.61.11.3645-3647.1987
更新日期:1987-11-01 00:00:00
abstract::Previous work by our laboratory and others has shown that mouse cells normally resistant to tumor necrosis factor can be made sensitive to the cytokine by the expression of adenovirus E1A. The E1A gene can be introduced by either infection or transfection, and either of the two major E1A proteins, 289R or 243R, can in...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.3.1236-1244.1991
更新日期:1991-03-01 00:00:00
abstract::Proteasome inhibitors (e.g., bortezomib, MG132) are known to enhance adeno-associated virus (AAV) transduction; however, whether this results from pleotropic proteasome inhibition or off-target serine and/or cysteine protease inhibition remains unresolved. Here, we examined recombinant AAV (rAAV) effects of a new prot...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01826-13
更新日期:2013-12-01 00:00:00
abstract::Enhancement of cell death is a distinguishing feature of H1N1 influenza virus A/Puerto Rico/8/34 protein PB1-F2. Comparing the sequences (amino acids [aa] 61 to 87 using PB1-F2 amino acid numbering) of the PB1-F2-derived C-terminal peptides from influenza A viruses inducing high or low levels of cell death, we identif...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01373-13
更新日期:2014-01-01 00:00:00
abstract::Enzymatic markers and electron microscopy were utilized to determine the cellular origin of the membrane types isolated from type 2 dengue virus-infected BHK cells by discontinuous sucrose gradient centrifugation. The results showed an apparent separation of plasma membrane, smooth and rough endoplasmic reticulum with...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.4.1017-1026.1975
更新日期:1975-10-01 00:00:00
abstract::HLA-B*57 is strongly associated with immune control of HIV and delayed AIDS progression. The closely related, but less protective, HLA-B*58:01 presents similar epitopes, but HLA-B*58:01(+) individuals do not generate CD8(+) T cells targeting the KF11-Gag epitope, which has been linked to low viremia. Here we show that...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01122-13
更新日期:2013-10-01 00:00:00
abstract::The human immunodeficiency virus type 1 (HIV-1) V3 loop is essential for coreceptor binding and principally determines tropism for the CCR5 and CXCR4 coreceptors. Using the dual-tropic virus HIV-1(R3A), we previously made an extensive panel of V3 deletions and identified subdomains within V3 that could differentially ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01751-08
更新日期:2009-04-01 00:00:00
abstract::HIV-1 causes a persistent infection of the immune system that is associated with chronic comorbidities. The mechanisms that underlie this inflammation are poorly understood. Emerging literature has implicated proinflammatory purinergic receptors and downstream signaling mediators in HIV-1 infection. This study probed ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01186-18
更新日期:2018-12-10 00:00:00
abstract::Hepatitis C virus (HCV) nonstructural protein 4B (NS4B) is an integral membrane protein, which plays an important role in the organization and function of the HCV replication complex (RC). Although much is understood about its amphipathic N-terminal and C-terminal domains, we know very little about the role of the tra...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02298-10
更新日期:2011-07-01 00:00:00
abstract::The amphotropic murine leukemia virus (MuLV) can infect cells from a number of mammals, including humans, via its specific receptor. Basic knowledge of amphotropic MuLV receptor expression is likely to be useful in the development and improvement of gene therapy protocols based on amphotropic-pseudotyped vectors. To i...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.71.6.4564-4570.1997
更新日期:1997-06-01 00:00:00
abstract::Infection of microglia and other cells of the macrophage/monocyte lineage in the central nervous system (CNS) by human immunodeficiency virus type I (HIV-1) underlies the development of giant cell encephalitis (GCE). It is currently unknown whether GCE depends on the emergence of virus populations specifically adapted...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.75.23.11686-11699.2001
更新日期:2001-12-01 00:00:00
abstract::We report the identification of a functional nuclear localization signal (NLS) in the human cytomegalovirus (HCMV) large tegument protein pUL48 that is required for nuclear localization in transfected cells and is essential for viral growth. The NLS was mapped to pUL48 amino acid residues 284 to 302. This sequence con...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03558-12
更新日期:2013-05-01 00:00:00
abstract::A detailed kinetic and quantitative analysis of the early and late biosynthetic events undergone by the human immunodeficiency virus type 1 envelope protein expressed by a recombinant vaccinia virus was performed. Early folding events that occurred in the endoplasmic reticulum included disulfide bond formation (t1/2 a...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.65.4.2047-2055.1991
更新日期:1991-04-01 00:00:00
abstract::The major histocompatibility complex (MHC) class I region of humans, chimpanzees (Pan troglodytes), and bonobos (Pan paniscus) is highly similar, and orthologues of HLA-A, -B, and -C are present in both Pan species. Based on functional characteristics, the different HLA-A allotypes are classified into different supert...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.01960-19
更新日期:2020-04-16 00:00:00
abstract::The major phosphoprotein common to woolly monkey sarcoma virus, gibbon ape lymphosarcoma virus, and type C viruses of the lower mammalian species (mouse, rat, cat), with the exception of the endogenous cat virus (RD-114), is the polypeptide of about 12,000 molecular weight. The protein-phosphate bond in this polypepti...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.16.1.123-131.1975
更新日期:1975-07-01 00:00:00
abstract:UNLABELLED:Varicella-zoster virus (VZV) is an alphaherpesvirus that causes varicella and herpes zoster. Membrane fusion is essential for VZV entry and the distinctive syncytium formation in VZV-infected skin and neuronal tissue. Herpesvirus fusion is mediated by a complex of glycoproteins gB and gH-gL, which are necess...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.00792-16
更新日期:2016-07-27 00:00:00
abstract:UNLABELLED:A balance between the functions of the influenza virus surface proteins hemagglutinin (HA) and neuraminidase (NA) is thought to be important for the transmission of viruses between humans. Here we describe two pandemic H1N1 viruses, A/swine/Virginia/1814-1/2012 and A/swine/Virginia/1814-2/2012 (pH1N1low-1 an...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.02537-14
更新日期:2015-06-01 00:00:00
abstract::Long-term ovine fibroblast cultures contain replicative-competent lentiviruses in a latent form. This in vitro phenomenon, never described previously for lentiviruses, was clearly demonstrated by activating the expression of latent viruses with various inducing cell treatments, some of which were efficient in inducing...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.52.2.680-682.1984
更新日期:1984-11-01 00:00:00
abstract:UNLABELLED:Uracil DNA glycosylases (UNG) are highly conserved proteins that preserve DNA fidelity by catalyzing the removal of mutagenic uracils. All herpesviruses encode a viral UNG (vUNG), and yet the role of the vUNG in a pathogenic course of gammaherpesvirus infection is not known. First, we demonstrated that the v...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.03111-14
更新日期:2015-03-01 00:00:00
abstract::Expression of the immediate-early (IE) genes of herpes simplex virus (HSV) is specifically stimulated by a 65-kilodalton virion transcription factor (VF65 or VP16) that is introduced as a component of infecting virions. In both the IE175(ICP4) and IE110(ICP0) promoters, this activation requires an upstream cis-acting ...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.63.6.2798-2812.1989
更新日期:1989-06-01 00:00:00
abstract::Here we show that the adenovirus early region 4 (E4) open reading frame 4 (ORF4) protein autoregulates its own transcription by inhibiting adenovirus E1A-induced activation of E4 transcription both in transient transfection experiments and during lytic virus growth. The inhibitory activity of E4-ORF4 was selective for...
journal_title:Journal of virology
pub_type: 杂志文章
doi:10.1128/JVI.70.6.3844-3851.1996
更新日期:1996-06-01 00:00:00