Abstract：
:Biofilm forming multidrug resistant Staphylococcus spp. are major reservoirs for transmission of ophthalmic infections. They were isolated from ocular patients suffering from conjunctivitis. In this study we analyzed biofilm forming ability, antibiotic resistance profile of the Staphylococcus spp. isolated from clinical ocular patients, and their phylogenetic relationship with other community MRSA. Sixty Staphylococcus spp. strains isolated from clinical subjects were evaluated for their ability to form biofilm and express biofilm encoding ica gene. Among them 93% were slime producers and 87% were slime positive. Staphylococcus aureus and S. epidermidis were dominant strains among the isolates obtained from ocular patients. The strains also exhibited a differential biofilm formation quantitatively. Antibiotic susceptibility of the strains tested with Penicillin G, Ciprofloxacin, Ofloxacin, Methicillin, Amikacin, and Gentamicin indicated that they were resistant to more than one antibiotic. The amplicon of ica gene of strong biofilm producing S. aureus strains, obtained by polymerase chain reaction, was sequenced and their close genetic relationship with community acquired MRSA was analyzed based on phylogenetic tree. Our results indicate that they are genetically close to other community acquired MRSA.
journal_name
Pol J Microbioljournal_title
Polish journal of microbiologyauthors
Murugan K,Usha M,Malathi P,Al-Sohaibani AS,Chandrasekaran Msubject
Has Abstractpub_date
2010-01-01 00:00:00pages
233-9issue
4eissn
1733-1331issn
2544-4646journal_volume
59pub_type
杂志文章abstract：:This study investigates the toxic effect of E(2)nonenal (trans-2-nonenal, T2N) and its conjugate with horse muscle myoglobin (Mb) tested on murine cell line L929 and human cell line A549, as well as the genotoxic effect of these compounds assayed by measuring of micronuclei in human cells K562. It is an aldehyde, whic...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:The oxazolidinone antimicrobial, linezolid, has been approved for the treatment of infections caused by various gram-positive bacteria, including vancomycin-resistant enterococci (VRE). This is the first report on isolation of a linezolid resistant, vancomycin-resistant Enterococcus faecium (LRVREF) strain in Poland, ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Pathogenicity and ultrastructural investigation of the inoculation of peppermint stems and rhizomes with Phoma strasseri conidia was undertaken using scanning and transmission electron microscopy to examine the host-parasite relationship. Pathogenicity experiments demonstrated that all tested P. strasseri isolates had...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:The Apicomplexa are a diverse group of intracellular parasitic protists. The majority of species from the classes Coccidea, Haemosporea and Piroplasmea are responsible for widespread diseases of humans and domestic animals. Oocysts of these parasites can persist for long periods of time in the environment (i.e. in wat...
journal_title：Polish journal of microbiology
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
abstract：:The effects of inorganic nutrients (N, P) enrichment of mesotrophic lake water on changes in bacterial and protistan (heterotrophic nanoflagellates and ciliates) communities compositions were studied in the mesocosm experiment. Phosphorus (PO4(3-)) and nitrogen (NH4+) alone and in combination were added to three types...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:In the present work primary antimicrobial resistance was analyzed in clinical H. pylori strain isolates from adult patients from Polish Wielkopolska region within the last 10 years. Drug sensitivity was evaluated in a total of 142 H. pylori isolates, with 66 strains originating from years 1997/1998 forming group 1 and...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:The aim of this research was to develop a suitable method of succinate dehydrogenase activity assay in situ for different industrial yeast strains. For this purpose different compounds: EDTA, Triton X-100, sodium deoxycholate, digitonin, nystatin and beta-mercaptoethanol were used. The permeabilization process was con...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Bovine mastitis caused by Prototheca spp. can be a disease of high significance because of economic losses and the potential risk to public health. The aim of our study was to evaluate enzymatic activity of Prototheca zopfii. For this study, we used 15 P. zopfii strains previously isolated from cows with clinical and ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2012-09-28 00:00:00
abstract：:A multiplex PCR system was developed for specific identification of genes encoding heat-labile (LTI) and heat-stable (STI and STII) toxins of enterotoxigenic Escherichia coli (ETEC) strains. In addition, primers specific for the E. coli gene coding for 16S rRNA were used as an internal control of the DNA amplification...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:The class Acidithiobacillia was established using multiprotein phylogenetic analysis of all the available genomes of the genus Acidithiobacillus (comprising Family I, the Acidithiobacillaceae, of the Acidithiobacillales, the order created for Bergey's Manual of Systematic Bacteriology), and for representative genomes ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:Bacteria play a fundamental role in the cycling of nutrients in aquatic environments. A precise distinction between active and inactive bacteria is crucial for the description of this process. We have evaluated the usefulness of Coomassie Blue G250 for fluorescent staining of protein containing potentially highly acti...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.4867
更新日期：2017-09-27 00:00:00
abstract：:We have reported a bacterial infection in a dog with progressive dysplasia of the hips. Orthopedic surgery was performed. Seven weeks prior to the surgery, the patient was bitten by another dog. The postimplantation wound exuded for four days after the surgery. Microbiological analysis performed by standard identifica...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
abstract：:The use of microbial exopolysaccharides (EPS) in the food, pharmaceutical, and chemical industries has steadily increased during the past decade. A bioactive EPS producing microorganism, Streptomyces nasri was isolated from Kuwait tropical soil and the proteopolysaccharide was tested for its antimicrobial activity. Th...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Alternaria alternata were isolated and identified from root, foliage and soil of both wheat and rice crops and their aggressiveness was studied using aggressiveness analysis. Isolates of Alternaria alternata were genetically characterized using RAPD's. The investigations were based on surveys of wheat and rice crops i...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:The aim of the study was to analyze the differences in the activity of beta-glucuronidase and beta-glucosidase in stool specimens of children with Inflammatory Bowel Diseases (IBD) and healthy subjects. The disease activity was determined according to the PCDAI scale (Crohn disease) and Truelove-Witts scale (Ulcerativ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:Several studies have observed that a conventional PCR protocol using primers LM1 and LM2 for the identification of gene hlyA Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason in this study, we provide a modified PCR protocol that improves the specificity of the LM1 a...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.7869
更新日期：2017-07-06 00:00:00
abstract：:Biologic prosthetics are increasingly used for the repair of abdominal wall hernia defects but can become infected as a result of peri- or early post-operative bacterial contamination. Data evaluating biofilm formation on biologic prosthetics is lacking. The aim of this study was to investigate the influence of differ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:Biofilms formed by nosocomial pathogens represent a major threat to patients undergoing invasive procedures. As prophylaxis remains the most efficient anti-biofilm option, it is of paramount importance to develop diagnostic tools able to detect biofilm at the early stage of formation. The present study investigates th...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：:The haloarchaeal diversity of a salt mine, a natural cave in central Anatolia, was investigated using convential microbiological and molecular biology methods. Eight halophilic archaeal isolates selected based on their colony morphology and whole cell protein profiles were taxonomically classified on the basis of thei...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:The antibacterial activities of five antibiotics, three brands of Ofloxacins (Obenasin, Floxavid and Drovid) and two brands of Ciprofloxacins (Uroxin and Siprosan), and five commonly used disinfectants (Lysol, Dettol, Purit, Roberts and Wex-cide) against Staphylococcus aureus, Escherichia coli, Proteus spp., Pseudomon...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Over the last two decades virus-like particles (VLPs) have become an important tool in biomedical research and medicine. VLPs are multiprotein structures that resemble viable virus particles in conformation but lack the viral genome. Consequently, they are non-infectious and non-replicative, but retain the ability to ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章,评审
doi：
更新日期：2015-01-01 00:00:00
abstract：:The expiration date of biopesticidal products is an essential feature of their use and storage. In the present work, the expiration date of biocontrol agent was predicted using the accelerated aging method. The available bacteria in Bacillus subtilis B579 biocontrol agent were 3.7 ± 0.2 × 1011 CFU/g. It is calculated ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1227672
更新日期：2017-01-02 00:00:00
abstract：:It is estimated that one third of the world's population have latent tuberculosis infection and that this is a significant reservoir for future tuberculosis cases. Most cases occur within two years following initial infection. The identification of individuals with latent tuberculosis infection is difficult due to the...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1234987
更新日期：2017-03-30 00:00:00
abstract：:The purpose of the study was to evaluate the usefulness of sonication for the diagnosis of prosthetic joint infections (PJIs) by its comparison with periprosthetic tissues (PTs) and synovial fluid (SV-F) cultures. The study groups included 54 patients undergoing exchange of total hip prostheses for so called "aseptic"...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:Of 175 Clostridium difficile strains isolated from patient hospitalized in one academic hospital in Warsaw between 2005-2006, one isolate belonged to PCR-ribotype 027/toxinotype III. This isolate had tcdA, tcdB, binary toxin genes (cdtA and cdtB), a 18-bp deletion and a 1 bp deletion at 117 position in the tcdC gene. ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Two bacterial strains identified as Ralstonia picketti (BP 20) and Alcaligenes piechaudii (CZOR L-1B) were isolated from petroleum hydrocarbon-contaminated soil following bioremediation treatment. The surface active properties, e.g. surface tension, emulsification and foamability of their culture filtrates were evalua...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Four ethanologenic ethanol-tolerant yeast strains, Saccharomyces cerevisiae (ATKU132), Saccharomycodes ludwigii (ATKU47), and Issatchenkia orientalis (ATKU5-60 and ATKU5-70), were isolated by an enrichment technique in yeast extract peptone dextrose (YPD) medium supplemented with 10% (v/v) ethanol at 30°C. Among non-S...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2012-09-28 00:00:00
abstract：:The mannosylated lipoarabinomanan (ManLAM) from mycobacterial species possesses strong anti-apoptotic action. Here we examined the ability of ManLAM isolated from Mycobacterium tuberculosis H37Rv to alter expression profiles of apoptosis-related genes in mouse macrophages infected with Mycobacterium bovis BCG Danish s...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:The aim of this study was to develop a non-invasive diagnostic test for the detection of Helicobacter pylori in stool samples from digestive symptomatic patients, using a new protocol of nested-qPCR. A total of 143 patients were invited to participate in the study. A gastric biopsy of each patient was collected for Ra...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0011.5881
更新日期：2018-03-09 00:00:00
abstract：:The synthesis and antimicrobial activity of 31 morpholinium chlorides, divided into five series depending on the substituents attached to the nitrogen atom, N-carboxyalkyl-morpholinium chlorides (1a-e), N-carbalkoxymethyl-N-methyl-morpholinium chlorides (2a-f), N-carbethoxymethyl-N-alkyl-morpholinium chlorides (3a-g),...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
