The Application of Impedance Microsensors for Real-Time Analysis of Pseudomonas aeruginosa Biofilm Formation.

Abstract:

:Biofilms formed by nosocomial pathogens represent a major threat to patients undergoing invasive procedures. As prophylaxis remains the most efficient anti-biofilm option, it is of paramount importance to develop diagnostic tools able to detect biofilm at the early stage of formation. The present study investigates the ability of impedance microsensors to detect Pseudomonas aeruginosa biofilm presence using the impedance spectroscopy method. The measured data were analyzed using Electrical Equivalent Circuit modelling (EEC). It allowed to recognize conduction and polarization phenomena on the sensors surface and in its environment. The impedance assay results, confirmed by means of electron microscopy and quantitative cultures, indicate that specific EEC parameters may be used for monitoring the development of pseudomonal biofilm.

journal_name

Pol J Microbiol

authors

Chabowski K,Junka AF,Szymczyk P,Piasecki T,Sierakowski A,Mączynska B,Nitsch K

subject

Has Abstract

pub_date

2015-01-01 00:00:00

pages

115-20

issue

2

eissn

1733-1331

issn

2544-4646

journal_volume

64

pub_type

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