Abstract:
:A multiplex PCR system was developed for specific identification of genes encoding heat-labile (LTI) and heat-stable (STI and STII) toxins of enterotoxigenic Escherichia coli (ETEC) strains. In addition, primers specific for the E. coli gene coding for 16S rRNA were used as an internal control of the DNA amplification. The specificity of the method was validated by single PCR tests performed with reference to E. coli strains as well as pig-isolated bacteria and 100% correlation was observed. The developed multiplex PCR allowed rapid and specific identification of enterotoxin-positive E. coli and may be used as a sensitive and specific method for a direct determination of ETEC and to differentiate them from other E. coli isolates.
journal_name
Pol J Microbioljournal_title
Polish journal of microbiologyauthors
Weiner M,Osek Jsubject
Has Abstractpub_date
2004-01-01 00:00:00pages
7-10issue
1eissn
1733-1331issn
2544-4646journal_volume
53pub_type
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
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更新日期:2005-01-01 00:00:00
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journal_title:Polish journal of microbiology
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更新日期:2004-01-01 00:00:00
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pub_type: 杂志文章
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journal_title:Polish journal of microbiology
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更新日期:2011-01-01 00:00:00
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journal_title:Polish journal of microbiology
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journal_title:Polish journal of microbiology
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更新日期:2010-01-01 00:00:00
abstract::The aim of the study was to determine the influence of the source material and the applied S. cerevisiae strain on the concentrations of carbonyl fractions in raw spirits. Acetaldehyde was the most common aldehyde found, as it accounted for 88-92% of the total amount of aldehydes. The concentration of acetaldehyde in ...
journal_title:Polish journal of microbiology
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.21307/pjm-2018-059
更新日期:2018-01-01 00:00:00
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journal_title:Polish journal of microbiology
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doi:
更新日期:2015-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
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更新日期:2012-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::Enterococcal infection has become a major clinical problem and E. faecalis and E. faecium are the most frequently isolated species. However, the isolation of other species (E. casseliflavus, E. gallinarum, E. durans) from clinical materials was reported recently. The aim of this study was to evaluate drug resistance o...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2012-09-28 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.5604/01.3001.0010.7869
更新日期:2017-07-06 00:00:00
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journal_title:Polish journal of microbiology
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更新日期:2011-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.5604/17331331.1197281
更新日期:2016-01-01 00:00:00
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journal_title:Polish journal of microbiology
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更新日期:2015-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.5604/01.3001.0011.6148
更新日期:2018-03-09 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2020-001
更新日期:2020-09-01 00:00:00
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journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00