Abstract：
:Several studies have observed that a conventional PCR protocol using primers LM1 and LM2 for the identification of gene hlyA Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason in this study, we provide a modified PCR protocol that improves the specificity of the LM1 and LM2 primers.
journal_name
Pol J Microbioljournal_title
Polish journal of microbiologyauthors
Godínez-Oviedo A,Nava GM,Arvizu-Medrano SM,Hernández-Iturriaga Mdoi
10.5604/01.3001.0010.7869subject
Has Abstractpub_date
2017-07-06 00:00:00pages
255-257issue
2eissn
1733-1331issn
2544-4646pii
1242033journal_volume
66pub_type
杂志文章abstract：:The objective of this study was to determine the effect of acid adaptation conditions on heat resistance of E. coli O157: H7 932. E. coli O157: H7 was adapted to acid by exposing the cells to pH 4.5 (2h), pH 5.0 (1 h), and pH 5.5 (1 h) in tryptic soy broth. D and z values of the acid adapted and control cultures at 54...
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abstract：:Molecular diversity studies of 19 rhizobia isolates from chickpea were conducted using simple sequence repeats (SSR) and 16S rDNA-RFLP markers. Phenotypic characterization with special reference to salinity and pH tolerance was performed. These isolates were identified as different strains of Mesorhizobium, Rhizobium,...
journal_title：Polish journal of microbiology
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abstract：:Paeonia ostii is known for its excellent medicinal values as Chinese traditional plant. To date, the diversity of culturable endophytes associated with P. ostii is in its initial phase of exploration. In this study, 56 endophytic bacteria and 51 endophytic fungi were isolated from P. ostii roots in China. Subsequent c...
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1204486
更新日期：2016-06-07 00:00:00
abstract：:Abortion in small ruminants is a significant problem in Iraq and causes severe economic losses in sheep farms. Chlamydia abortus causes enzootic abortion in ewes and is associated with reproductive problems in sheep in Sulaimani province - Northern Iraq. During a lambing season in 2017, abortion was widespread among s...
journal_title：Polish journal of microbiology
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doi：10.33073/pjm-2020-009
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doi：10.21307/pjm-2019-017
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abstract：:The aim of the study was evaluation of qualitative and quantitative changes in bacterial ecosystem in 109 children with inflammatory bowel diseases. Stools obtained from patients were analysed for selected bacteria and concentration of faecal inflammatory markers (calprotectin, lactoferrin, M2-PK). The number of selec...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：:Quorum sensing (QS), or cell-to-cell communication in bacteria, is achieved through the production and subsequent response to the accumulation of extracellular signal molecules called autoinductors. The main role of QS is regulation of production of virulence factors in bacteria. Bacterial pathogenicity is often manif...
journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Neutralization method evaluation is an important first step in a disinfectant validation study program. It is also crucial in assessment of the efficiency of microbial recovery media in the presence of a residual biocidal agent. In the present study, four commercially available peroxygen/silver-based disinfectant form...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1197271
更新日期：2016-01-01 00:00:00
abstract：:Pathogenicity and ultrastructural investigation of the inoculation of peppermint stems and rhizomes with Phoma strasseri conidia was undertaken using scanning and transmission electron microscopy to examine the host-parasite relationship. Pathogenicity experiments demonstrated that all tested P. strasseri isolates had...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:The studies have been performed to analyze the production of beta-glucanase by a recombinant strain of Escherichia coli immobilized in different matrices. Porous sintered glass SIRAN, Ceramic supporting matrices and Broken Pumice stone as well as SIRAN Raschig-rings were examined for the immobilization of whole bacter...
journal_title：Polish journal of microbiology
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doi：
更新日期：2011-01-01 00:00:00
abstract：:To understand the molecular epidemiology and antibiotic resistance of air and clinical isolates of Acinetobacter baumannii , the intensive care unit settings of a hospital in Northern China were surveyed in 2014. Twenty non-duplicate A. baumannii isolates were obtained from patients and five isolates of airborne A. ba...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.21307/pjm-2018-040
更新日期：2018-01-01 00:00:00
abstract：:In this study, we revealed rapid detection of streptomycin-producing Streptomyces spp. by extraction of total soil DNA from 14 soil samples using a modified lysis method followed by PCR amplification ofa genus-specific sequence in the Streptomyces' 16S rDNA gene. DNA band of the expected size (438 bp) was seen with al...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章,评审
doi：
更新日期：2008-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.4867
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abstract：:The current "gold standard" in molecular epidemiological studies of Mycobacterium tuberculosis is IS6110 RFLP based on IS6110 polymorphism. However PCR-based methods are becoming increasingly important. Recently, fast ligation-mediated PCR (FLiP), based on IS6110 polymorphism was proposed. In this study, the discrimin...
journal_title：Polish journal of microbiology
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doi：
更新日期：2013-01-01 00:00:00
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更新日期：2010-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:We report the interspecies transfer of the bla(VLM-4) gene in MBL-producing Enterobacter cloacae and Klebsiella pneumoniae isolates from a newborn patient who had received meropenem therapy. We show evidence that gene bla(VIM-4) was transmitted as a part of the class-1 integron on a ca. -90 kb conjugative plasmid. Hig...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1185239
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abstract：:To determine the staphylococcal colonization pattern in healthy and diseased dogs, living in two particular environments, a number of microbiological samples were taken. Overall, twenty dogs, either healthy or with infected skin lesions, were examined. In each case bacterial swabs were collected from the nasal mucosa,...
journal_title：Polish journal of microbiology
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doi：
更新日期：2011-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1227672
更新日期：2017-01-02 00:00:00
abstract：:Bacillus subtilis strains (CM1-CM5) isolated from culturable cowdung microflora were investigated for indole-3-acetic acid (IAA) production in nutrient broth (NB). All the strains tested produced IAA in NB; albeit in very low concentrations (0.09-0.37 mg/l). The addition of L-tryptophan (0.1 - 1.0 g/l) into NB substan...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:Proteus mirabilis isolates (n = 177), collected between 1996 and 2000 in four hospitals in the West Pomeranian area of Poland, were characterized by antibiotype and pulsed-field gel electrophoresis (PFGE). The selected isolates were collected from different wards (intensive care unit, surgery, internal medicine, and u...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:Clinical diagnosis of hepatitis E viral (HEV) infection mainly relies on serological assays, and the current status of misdiagnoses regarding HEV infection is uncertain. In this study, patients with acute HEV infection were tested for anti-HEV IgM and IgG, a HEV antigen (Ag), and viral loads (HEV RNA). Serology was pe...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.33073/pjm-2020-025
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