Abstract：
:The current "gold standard" in molecular epidemiological studies of Mycobacterium tuberculosis is IS6110 RFLP based on IS6110 polymorphism. However PCR-based methods are becoming increasingly important. Recently, fast ligation-mediated PCR (FLiP), based on IS6110 polymorphism was proposed. In this study, the discriminatory power of FLIP, spoligotyping and MIRU-VNTR typing, in differentiation of M. tuberculosis isolates was compared. The discriminatory index (HGI) of spoligotyping, MIRU-VNTR analysis, and FLiP was 0.653, 0.837, and 0.917, respectively. This indicates that FLiP allows a high level of differentiation among M. tuberculosis strains and it might be a valuable alternative to the other typing methods.
journal_name
Pol J Microbioljournal_title
Polish journal of microbiologyauthors
Zaczek A,Brzostek A,Górna A,Sajduda A,Wojtasik A,Dziadek Jsubject
Has Abstractpub_date
2013-01-01 00:00:00pages
73-6issue
1eissn
1733-1331issn
2544-4646journal_volume
62pub_type
杂志文章abstract：:The aim of this study was to develop a non-invasive diagnostic test for the detection of Helicobacter pylori in stool samples from digestive symptomatic patients, using a new protocol of nested-qPCR. A total of 143 patients were invited to participate in the study. A gastric biopsy of each patient was collected for Ra...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0011.5881
更新日期：2018-03-09 00:00:00
abstract：:Despite the enormous progress in understanding the process of bacterial pathogenesis and interactions of pathogens with eucaryotic cells the infectious diseases still remain the main cause of human premature deaths. It is now recognized that Helicobacter pylori infects about half of the world's population. Based on re...
journal_title：Polish journal of microbiology
pub_type： 杂志文章,评审
doi：
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abstract：:In the present work primary antimicrobial resistance was analyzed in clinical H. pylori strain isolates from adult patients from Polish Wielkopolska region within the last 10 years. Drug sensitivity was evaluated in a total of 142 H. pylori isolates, with 66 strains originating from years 1997/1998 forming group 1 and...
journal_title：Polish journal of microbiology
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doi：
更新日期：2009-01-01 00:00:00
abstract：:The diagnostic assessment of water sanitary state is based mainly on the cultivation of bacteria retained on membrane filters. However classical microbiology methods have a lot of disadvantages. More and more frequently, rapid detection and identification of pathogens present in water is based on molecular biology tec...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.4861
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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更新日期：2004-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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更新日期：2014-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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更新日期：2007-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.7869
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.7858
更新日期：2017-07-06 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:In this research, Salmonella species were isolated from the animal, insect and human enteric sources in Faisalabad, Punjab, Pakistan. These species were characterized by different microbiological and molecular techniques including polymerase chain reaction (PCR) by amplification of the 16S rRNA gene. Furthermore, sequ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.21307/pjm-2019-017
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.4855
更新日期：2017-09-27 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：:A series of N-substituted N'-(2-alkylthio-4-chloro-5-methylbenzenesulfonyl)guanidine derivatives bearing sulfonamide moiety have been screened in vitro for antibacterial activity against isolates from patients with infections of oral cavity, respiratory tract and intestinal tract. The majority of compounds exhibited g...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
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journal_title：Polish journal of microbiology
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doi：
更新日期：2010-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章,评审
doi：
更新日期：2015-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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更新日期：2010-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1197281
更新日期：2016-01-01 00:00:00
