Abstract：
:The objective of this study was to determine the effect of acid adaptation conditions on heat resistance of E. coli O157: H7 932. E. coli O157: H7 was adapted to acid by exposing the cells to pH 4.5 (2h), pH 5.0 (1 h), and pH 5.5 (1 h) in tryptic soy broth. D and z values of the acid adapted and control cultures at 54 degrees C, 56 degrees C, and 58 degrees C were determined in E buffer. The heat resistance of E. coli O157: H7 increased significantly (p<0.05) after acid adaptation at pH 4.5 or pH 5.0. E. coli O157: H7 adapted to acid at pH 4.5 for 2 h had the highest D values at all temperatures tested (20.3-10.7-3.3 min) while D values of culture adapted to acid at pH 5.0 for 1 h were 18.2, 7.9, and 2.6 min at 54 degrees C, 56 degrees C and 58 degrees C, respectively. Heat resistance of culture adapted to acid at pH 5.5 for 1 h and the control culture was not significantly different (P<0.05). Culture adapted to acid at pH 4.5 had the highest z value (5.10 degrees C), whereas control culture had the lowest z value (4.33 degrees C). This study showed that the magnitude of heat tolerance changed with the adaptation pH and at low adaptation pH, E. coli O157: H7 showed maximum heat resistance. Acid adaptation at pH 4.5 or 5.0 provides E. coli O157: H7 with cross-protection against heat treatments, and that this factor must be considered to estimate this pathogen's thermal tolerance accurately.
journal_name
Pol J Microbioljournal_title
Polish journal of microbiologyauthors
Tosun H,Gönül SAsubject
Has Abstractpub_date
2005-01-01 00:00:00pages
295-9issue
4eissn
1733-1331issn
2544-4646journal_volume
54pub_type
杂志文章abstract：:The aim of the study was to determine the influence of the presence or the absence of antibiotic input on the emergence and maintenance of resistance in commensal bacteria from food producing animals. The research material constituted E. coli isolates from two animal species: swine at different age from one convention...
journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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更新日期：2007-01-01 00:00:00
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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doi：
更新日期：2010-01-01 00:00:00
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
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更新日期：2008-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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更新日期：2012-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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更新日期：2009-01-01 00:00:00
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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更新日期：2011-01-01 00:00:00
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journal_title：Polish journal of microbiology
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journal_title：Polish journal of microbiology
pub_type： 杂志文章
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