Abstract:
:Two different extracellular lipases were isolated and purified from Pseudomonas aeruginosa Ps-x to apparent homogeneity using ammonium sulfate precipitation followed by ion exchange chromatography on Q- and S-Sepharose column. Both of the purified lipases are monomeric protein with molecular weight of 15.5 and 54.97 KDa respectively. The optimal activities of the enzymes were at 45 and 50 degrees C and pHs 10.0 and 9.0. Calcium ions increase thermostability of both purified lipases I and II. The purified lipase I showed no metal ion dependence for its activity since EDTA up to 10 mM has no effect on the enzyme activity. However purified lipase II showed slight inhibition by EDTA at the same concentration. Moreover, a serine protease inhibitor, PMSF showed an inhibitory effect on both purified enzymes.
journal_name
Pol J Microbioljournal_title
Polish journal of microbiologyauthors
Saeed HM,Zaghloul TI,Khalil AI,Abdelbaeth MTsubject
Has Abstractpub_date
2005-01-01 00:00:00pages
233-40issue
3eissn
1733-1331issn
2544-4646journal_volume
54pub_type
杂志文章abstract::To determine the staphylococcal colonization pattern in healthy and diseased dogs, living in two particular environments, a number of microbiological samples were taken. Overall, twenty dogs, either healthy or with infected skin lesions, were examined. In each case bacterial swabs were collected from the nasal mucosa,...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::The studies have been performed to analyze the production of beta-glucanase by a recombinant strain of Escherichia coli immobilized in different matrices. Porous sintered glass SIRAN, Ceramic supporting matrices and Broken Pumice stone as well as SIRAN Raschig-rings were examined for the immobilization of whole bacter...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Alternaria alternata were isolated and identified from root, foliage and soil of both wheat and rice crops and their aggressiveness was studied using aggressiveness analysis. Isolates of Alternaria alternata were genetically characterized using RAPD's. The investigations were based on surveys of wheat and rice crops i...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The aim of the study was to analyze the reasons for removing implants after breast reconstruction in the course of treatment of breast cancer. The study involved 428 patients, who underwent a total of 648 breast reconstruction procedures using artificial implants. 47 out of 648 cases (7.3%) were identified in which th...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2019-037
更新日期:2019-09-01 00:00:00
abstract::In vitro studies aimed at studying the mechanism of action of carvacrol and oregano as natural anti-bacterial agents to control multiple antibiotic-resistant avian pathogenic Escherichia coli (APEC) strain O23:H52 isolated from chicken were performed. Derivatives with increased minimum inhibitory concentrations (MIC) ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2020-003
更新日期:2020-01-01 00:00:00
abstract::In the present work primary antimicrobial resistance was analyzed in clinical H. pylori strain isolates from adult patients from Polish Wielkopolska region within the last 10 years. Drug sensitivity was evaluated in a total of 142 H. pylori isolates, with 66 strains originating from years 1997/1998 forming group 1 and...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::Clinical diagnosis of hepatitis E viral (HEV) infection mainly relies on serological assays, and the current status of misdiagnoses regarding HEV infection is uncertain. In this study, patients with acute HEV infection were tested for anti-HEV IgM and IgG, a HEV antigen (Ag), and viral loads (HEV RNA). Serology was pe...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2020-025
更新日期:2020-09-01 00:00:00
abstract::The aim of the study was to determine the influence of the presence or the absence of antibiotic input on the emergence and maintenance of resistance in commensal bacteria from food producing animals. The research material constituted E. coli isolates from two animal species: swine at different age from one convention...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::A disk-diffusion method experiment assessed the impact of nanosilver on production of secondary metabolites (pigments) by the Fusarium culmorum fungus. Nanosilver colloidal particles in water have been obtained by the use of a method based on high voltage electric arcs between silver electrodes. The silver nanoparticl...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::Growing cultures of the green obligate photolithotroph, Chlorobaculum parvum DSM 263T (formerly Chlorobium vibrioforme forma specialis thiosulfatophilum NCIB 8327), oxidized sulfide quantitatively to elemental sulfur, with no sulfate formation. In the early stages of growth and sulfide oxidation, the sulfur product be...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The aim of this research was to develop a suitable method of succinate dehydrogenase activity assay in situ for different industrial yeast strains. For this purpose different compounds: EDTA, Triton X-100, sodium deoxycholate, digitonin, nystatin and beta-mercaptoethanol were used. The permeabilization process was con...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::The objective of this study was to determine the effect of acid adaptation conditions on heat resistance of E. coli O157: H7 932. E. coli O157: H7 was adapted to acid by exposing the cells to pH 4.5 (2h), pH 5.0 (1 h), and pH 5.5 (1 h) in tryptic soy broth. D and z values of the acid adapted and control cultures at 54...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The composition of vagina lactic acid bacteria (LAB) differs within the different ethnic group. This study is aimed at determining the prevalence of LAB with their antimicrobial properties in Nigerian women's vagina during different stages of the menstrual cycle. Microorganisms were isolated from vaginal swabs of ten ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2019-020
更新日期:2019-01-01 00:00:00
abstract::The expiration date of biopesticidal products is an essential feature of their use and storage. In the present work, the expiration date of biocontrol agent was predicted using the accelerated aging method. The available bacteria in Bacillus subtilis B579 biocontrol agent were 3.7 ± 0.2 × 1011 CFU/g. It is calculated ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.5604/17331331.1227672
更新日期:2017-01-02 00:00:00
abstract::We have reported a bacterial infection in a dog with progressive dysplasia of the hips. Orthopedic surgery was performed. Seven weeks prior to the surgery, the patient was bitten by another dog. The postimplantation wound exuded for four days after the surgery. Microbiological analysis performed by standard identifica...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::Biologic prosthetics are increasingly used for the repair of abdominal wall hernia defects but can become infected as a result of peri- or early post-operative bacterial contamination. Data evaluating biofilm formation on biologic prosthetics is lacking. The aim of this study was to investigate the influence of differ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::This study was conducted to determine the prevalence of Campylobacter spp. isolated from dogs' faecal samples. From June 2012 to June 2013, a total of 210 faecal samples from pet dogs living in different kennels (n = 210) were collected by the owners in Greater Poland Voivodeship, Poznań District, Poland. The study re...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2015-01-01 00:00:00
abstract::Abortion in small ruminants is a significant problem in Iraq and causes severe economic losses in sheep farms. Chlamydia abortus causes enzootic abortion in ewes and is associated with reproductive problems in sheep in Sulaimani province - Northern Iraq. During a lambing season in 2017, abortion was widespread among s...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2020-009
更新日期:2020-01-01 00:00:00
abstract::The haloarchaeal diversity of a salt mine, a natural cave in central Anatolia, was investigated using convential microbiological and molecular biology methods. Eight halophilic archaeal isolates selected based on their colony morphology and whole cell protein profiles were taxonomically classified on the basis of thei...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::In this study, we revealed rapid detection of streptomycin-producing Streptomyces spp. by extraction of total soil DNA from 14 soil samples using a modified lysis method followed by PCR amplification ofa genus-specific sequence in the Streptomyces' 16S rDNA gene. DNA band of the expected size (438 bp) was seen with al...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::The activity of photosynthesizing sulfur bacteria in a continuous culture was studied. The bacteria were isolated from the natural environment with the use of the Winogradski column. Isolated bacteria were cultured in synthetic medium and in the effluent from the column containig HS-. Sulphides, the main product of re...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2007-01-01 00:00:00
abstract::Despite the enormous progress in understanding the process of bacterial pathogenesis and interactions of pathogens with eucaryotic cells the infectious diseases still remain the main cause of human premature deaths. It is now recognized that Helicobacter pylori infects about half of the world's population. Based on re...
journal_title:Polish journal of microbiology
pub_type: 杂志文章,评审
doi:
更新日期:2008-01-01 00:00:00
abstract::Coxiella burnetii is the etiologic agent of Q fever, a worldwide distributed zoonosis, accountable for serious health problem both for humans and animals. The exposure to C. burnetii infected animals and their products is the main risk factor for Q fever in humans. Several outbreaks of Q fever have been described in P...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The current "gold standard" in molecular epidemiological studies of Mycobacterium tuberculosis is IS6110 RFLP based on IS6110 polymorphism. However PCR-based methods are becoming increasingly important. Recently, fast ligation-mediated PCR (FLiP), based on IS6110 polymorphism was proposed. In this study, the discrimin...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2013-01-01 00:00:00
abstract::In this research, Salmonella species were isolated from the animal, insect and human enteric sources in Faisalabad, Punjab, Pakistan. These species were characterized by different microbiological and molecular techniques including polymerase chain reaction (PCR) by amplification of the 16S rRNA gene. Furthermore, sequ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.21307/pjm-2019-017
更新日期:2019-01-01 00:00:00
abstract::Several studies have observed that a conventional PCR protocol using primers LM1 and LM2 for the identification of gene hlyA Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason in this study, we provide a modified PCR protocol that improves the specificity of the LM1 a...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.5604/01.3001.0010.7869
更新日期:2017-07-06 00:00:00
abstract::Many bacterial species can be a cause of various heart diseases, such as: Borrelia burgdorferi sensu lato, Coxiella burnetii and Bartonella spp. The aim of the present studies was to establish if any tick-borne infections can contribute to serious heart disorders resulting in the need for heart transplantation. Myocar...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Probiotics are considered an alternative to antibiotics in the prevention and treatment of Salmonella diseases in poultry. However, to use probiotics as proposed above, it is necessary to evaluate their properties in detail and to select the most effective bacterial strains in the application targeted. In this study, ...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:10.33073/pjm-2020-001
更新日期:2020-09-01 00:00:00
abstract::The aim of this study was to evaluate the effect of ciprofloxacin (CIP), N-acetylcysteine (NAC) alone and in combination on biofilm production and pre-formed mature biofilms on ureteral stent surfaces. Two strains each of Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, Klebseilla pneumoniae, Pseud...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00
abstract::The synthesis and antimicrobial activity of 31 morpholinium chlorides, divided into five series depending on the substituents attached to the nitrogen atom, N-carboxyalkyl-morpholinium chlorides (1a-e), N-carbalkoxymethyl-N-methyl-morpholinium chlorides (2a-f), N-carbethoxymethyl-N-alkyl-morpholinium chlorides (3a-g),...
journal_title:Polish journal of microbiology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00