Purification and characterization of two extracellular lipases from Pseudomonas aeruginosa Ps-x.

Abstract:

:Two different extracellular lipases were isolated and purified from Pseudomonas aeruginosa Ps-x to apparent homogeneity using ammonium sulfate precipitation followed by ion exchange chromatography on Q- and S-Sepharose column. Both of the purified lipases are monomeric protein with molecular weight of 15.5 and 54.97 KDa respectively. The optimal activities of the enzymes were at 45 and 50 degrees C and pHs 10.0 and 9.0. Calcium ions increase thermostability of both purified lipases I and II. The purified lipase I showed no metal ion dependence for its activity since EDTA up to 10 mM has no effect on the enzyme activity. However purified lipase II showed slight inhibition by EDTA at the same concentration. Moreover, a serine protease inhibitor, PMSF showed an inhibitory effect on both purified enzymes.

journal_name

Pol J Microbiol

authors

Saeed HM,Zaghloul TI,Khalil AI,Abdelbaeth MT

subject

Has Abstract

pub_date

2005-01-01 00:00:00

pages

233-40

issue

3

eissn

1733-1331

issn

2544-4646

journal_volume

54

pub_type

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