Abstract：
:Molecular diversity studies of 19 rhizobia isolates from chickpea were conducted using simple sequence repeats (SSR) and 16S rDNA-RFLP markers. Phenotypic characterization with special reference to salinity and pH tolerance was performed. These isolates were identified as different strains of Mesorhizobium, Rhizobium, Bradyrhizobium, and Agrobacterium. Twenty SSR loci of Mesorhizobium ciceri, distributed across the other rhizobial genome, clearly differentiated 19 rhizobial isolates. Analogous clustering supported the results of 16S rDNA sequence-based phylogeny. Analysis of the 16S rDNA sequences from M. ciceri strains revealed that nucleotide variables (signature sites) were located at 20 different positions; most of them were present in the first 820 bp region from 5' terminal. Interestingly, 14 signature sites were located in two main regions, the variable region V1 (nt 527-584), and variable region V2 (nt 754-813). The secondary structure and minimal free energy were determined in these two regions. These results will be useful in characterizing the micro-evolutionary mechanisms of species formation and increase understanding of the symbiotic relationship.
journal_name
Pol J Microbioljournal_title
Polish journal of microbiologyauthors
Yadav A,Singh AL,Rai GK,Singh Msubject
Has Abstractpub_date
2013-01-01 00:00:00pages
253-62issue
3eissn
1733-1331issn
2544-4646journal_volume
62pub_type
杂志文章abstract：:In this study we asked a question whether H. pylori LPS with or without LewisXY (Le) determinants as well as LBP (lipopolysaccharide binding protein) and sCD 14 molecules recognizing bacterial LPS may be involved in atherogenesis. Sera from 57 patients with coronary heart disease (CHD), 27 H. pylori infected dyspeptic...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:We evaluated the usefulness of the Carba NP test for rapid detection of carbapenemase activity in Bacteroides spp. The minimum inhibitory concentration (MIC) for imipenem was determined with gradient test strips, and cfiA gene was investigated by polymerase chain reaction for 27 clinical Bacteroides spp. isolates. Car...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0011.6148
更新日期：2018-03-09 00:00:00
abstract：:The synthesis and antimicrobial activity of 31 morpholinium chlorides, divided into five series depending on the substituents attached to the nitrogen atom, N-carboxyalkyl-morpholinium chlorides (1a-e), N-carbalkoxymethyl-N-methyl-morpholinium chlorides (2a-f), N-carbethoxymethyl-N-alkyl-morpholinium chlorides (3a-g),...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2010-01-01 00:00:00
abstract：:Several studies have observed that a conventional PCR protocol using primers LM1 and LM2 for the identification of gene hlyA Listeria monocytogenes generates non-specific PCR amplifications and false positives. For this reason in this study, we provide a modified PCR protocol that improves the specificity of the LM1 a...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.7869
更新日期：2017-07-06 00:00:00
abstract：:Pseudomonas aeruginosa has emerged as a major healthcare associated pathogen that creates a serious public health disaster in both developing and developed countries. In this work we aimed at studying the occurrence of metallo-beta-lactamase (MBL) producing P. aeruginosa in a healthcare setting in Alexandria, Egypt. T...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.4855
更新日期：2017-09-27 00:00:00
abstract：:A total 44 isolates of S. enterica serovar Enteritidis (S. Enteritidis) belonged to three different phage types (PTs) 9a, 13a, 25 were analyzed by the technique of pulsed-field gel electrophoresis (PFGE). Thirty and three strains were from two outbreaks (central and southern regions of the Slovak Republic PTs 13a, 25)...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:Many bacterial species can be a cause of various heart diseases, such as: Borrelia burgdorferi sensu lato, Coxiella burnetii and Bartonella spp. The aim of the present studies was to establish if any tick-borne infections can contribute to serious heart disorders resulting in the need for heart transplantation. Myocar...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：:The aim of the study was to determine the influence of the source material and the applied S. cerevisiae strain on the concentrations of carbonyl fractions in raw spirits. Acetaldehyde was the most common aldehyde found, as it accounted for 88-92% of the total amount of aldehydes. The concentration of acetaldehyde in ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.4861
更新日期：2017-09-27 00:00:00
abstract：:The mannosylated lipoarabinomanan (ManLAM) from mycobacterial species possesses strong anti-apoptotic action. Here we examined the ability of ManLAM isolated from Mycobacterium tuberculosis H37Rv to alter expression profiles of apoptosis-related genes in mouse macrophages infected with Mycobacterium bovis BCG Danish s...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:In the present work primary antimicrobial resistance was analyzed in clinical H. pylori strain isolates from adult patients from Polish Wielkopolska region within the last 10 years. Drug sensitivity was evaluated in a total of 142 H. pylori isolates, with 66 strains originating from years 1997/1998 forming group 1 and...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2009-01-01 00:00:00
abstract：:Biofilms formed by nosocomial pathogens represent a major threat to patients undergoing invasive procedures. As prophylaxis remains the most efficient anti-biofilm option, it is of paramount importance to develop diagnostic tools able to detect biofilm at the early stage of formation. The present study investigates th...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：:Two hundred and fifty bacterial strains were isolated from pinyon rhizosphere and screened for biosurfactants production. Among them, six bacterial strains were selected for their potential to produce biosurfactants using two low cost wastes, crude glycerol and lactoserum, as raw material. Both wastes were useful for ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2016-01-01 00:00:00
abstract：:It is estimated that one third of the world's population have latent tuberculosis infection and that this is a significant reservoir for future tuberculosis cases. Most cases occur within two years following initial infection. The identification of individuals with latent tuberculosis infection is difficult due to the...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1234987
更新日期：2017-03-30 00:00:00
abstract：:This study was conducted to determine the prevalence of Campylobacter spp. isolated from dogs' faecal samples. From June 2012 to June 2013, a total of 210 faecal samples from pet dogs living in different kennels (n = 210) were collected by the owners in Greater Poland Voivodeship, Poznań District, Poland. The study re...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：:Alternaria alternata were isolated and identified from root, foliage and soil of both wheat and rice crops and their aggressiveness was studied using aggressiveness analysis. Isolates of Alternaria alternata were genetically characterized using RAPD's. The investigations were based on surveys of wheat and rice crops i...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:The class Acidithiobacillia was established using multiprotein phylogenetic analysis of all the available genomes of the genus Acidithiobacillus (comprising Family I, the Acidithiobacillaceae, of the Acidithiobacillales, the order created for Bergey's Manual of Systematic Bacteriology), and for representative genomes ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2014-01-01 00:00:00
abstract：:The Apicomplexa are a diverse group of intracellular parasitic protists. The majority of species from the classes Coccidea, Haemosporea and Piroplasmea are responsible for widespread diseases of humans and domestic animals. Oocysts of these parasites can persist for long periods of time in the environment (i.e. in wat...
journal_title：Polish journal of microbiology
pub_type： 杂志文章,评审
doi：
更新日期：2004-01-01 00:00:00
abstract：:The aim of this study was to develop a non-invasive diagnostic test for the detection of Helicobacter pylori in stool samples from digestive symptomatic patients, using a new protocol of nested-qPCR. A total of 143 patients were invited to participate in the study. A gastric biopsy of each patient was collected for Ra...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0011.5881
更新日期：2018-03-09 00:00:00
abstract：:The aim of the study was to determine the influence of the presence or the absence of antibiotic input on the emergence and maintenance of resistance in commensal bacteria from food producing animals. The research material constituted E. coli isolates from two animal species: swine at different age from one convention...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:Mollusk shells provide a hard substrate for aquatic biofilm colonization. While most work has focused on bivalve shells and grazing, little work has focused on gastropod shells and the microbes growing on them. We sampled biofilms from 14 Pleuroceracanaliculatum and analyzed them using a metagenomic approach. Microbia...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2015-01-01 00:00:00
abstract：:The oxazolidinone antimicrobial, linezolid, has been approved for the treatment of infections caused by various gram-positive bacteria, including vancomycin-resistant enterococci (VRE). This is the first report on isolation of a linezolid resistant, vancomycin-resistant Enterococcus faecium (LRVREF) strain in Poland, ...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
abstract：:This study investigates the toxic effect of E(2)nonenal (trans-2-nonenal, T2N) and its conjugate with horse muscle myoglobin (Mb) tested on murine cell line L929 and human cell line A549, as well as the genotoxic effect of these compounds assayed by measuring of micronuclei in human cells K562. It is an aldehyde, whic...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2005-01-01 00:00:00
abstract：:Bacteria play a fundamental role in the cycling of nutrients in aquatic environments. A precise distinction between active and inactive bacteria is crucial for the description of this process. We have evaluated the usefulness of Coomassie Blue G250 for fluorescent staining of protein containing potentially highly acti...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/01.3001.0010.4867
更新日期：2017-09-27 00:00:00
abstract：:Neutralization method evaluation is an important first step in a disinfectant validation study program. It is also crucial in assessment of the efficiency of microbial recovery media in the presence of a residual biocidal agent. In the present study, four commercially available peroxygen/silver-based disinfectant form...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1197271
更新日期：2016-01-01 00:00:00
abstract：:The investigation was performed on 75 of Golden Retriever puppies. Faecal samples were collected on the 42 day of the puppies life (con-trol). Probiotic preparation was administered on 43 day of the puppies life and 10 days after the application of the probiotic, faecal samples were collected again (on 53 day of puppi...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：10.5604/17331331.1204486
更新日期：2016-06-07 00:00:00
abstract：:Pathogenicity and ultrastructural investigation of the inoculation of peppermint stems and rhizomes with Phoma strasseri conidia was undertaken using scanning and transmission electron microscopy to examine the host-parasite relationship. Pathogenicity experiments demonstrated that all tested P. strasseri isolates had...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2012-01-01 00:00:00
abstract：:Bacillus subtilis strains (CM1-CM5) isolated from culturable cowdung microflora were investigated for indole-3-acetic acid (IAA) production in nutrient broth (NB). All the strains tested produced IAA in NB; albeit in very low concentrations (0.09-0.37 mg/l). The addition of L-tryptophan (0.1 - 1.0 g/l) into NB substan...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2007-01-01 00:00:00
abstract：:The diagnostic assessment of water sanitary state is based mainly on the cultivation of bacteria retained on membrane filters. However classical microbiology methods have a lot of disadvantages. More and more frequently, rapid detection and identification of pathogens present in water is based on molecular biology tec...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2013-01-01 00:00:00
abstract：:The antibacterial activities of five antibiotics, three brands of Ofloxacins (Obenasin, Floxavid and Drovid) and two brands of Ciprofloxacins (Uroxin and Siprosan), and five commonly used disinfectants (Lysol, Dettol, Purit, Roberts and Wex-cide) against Staphylococcus aureus, Escherichia coli, Proteus spp., Pseudomon...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2008-01-01 00:00:00
abstract：:A multiplex PCR system was developed for specific identification of genes encoding heat-labile (LTI) and heat-stable (STI and STII) toxins of enterotoxigenic Escherichia coli (ETEC) strains. In addition, primers specific for the E. coli gene coding for 16S rRNA were used as an internal control of the DNA amplification...
journal_title：Polish journal of microbiology
pub_type： 杂志文章
doi：
更新日期：2004-01-01 00:00:00
