Abstract：
PURPOSE:To analyze the biological stability of autologous serum eyedrops after lyophilization. DESIGN:Prospective, comparative experimental study. METHODS:This was a comparative study with serum obtained from 12 healthy volunteers. The concentrations of different epitheliotropic factors (eg, transforming growth factor-β [TGF-β1], epidermal growth factor [EGF], platelet-derived growth factor AB [PDGF-AB], and albumin) were measured in fresh and lyophilized serum. The samples were studied after serum preparation (fresh serum) and immediately after saline solution reconstitution of lyophilized serum (0), 15, and 30 days later. The biological effects of both serum samples were also compared on conjunctival and corneal cell cultures. The pH, osmolarity, and serum density were also determined. RESULTS:No significant differences were found in the concentration of growth factors between fresh serum and re-dissolved serum samples after lyophilization. The concentration of growth factors remained stable during 1 month at 4°C in re-dissolved lyophilized form with saline solution. No differences were found related to osmolarity, pH, and density between fresh and lyophilized serum. In addition, no differences were found on the conjunctival and corneal cells proliferation and differentiation in cells cultures between either serum preparation. CONCLUSIONS:The properties of autologous serum remain after lyophilization. The lyophilized serum can be easily stored without temperature restrictions and easily reconstituted for preparation of eyedrops for standard clinical use.
journal_name
Am J Ophthalmoljournal_title
American journal of ophthalmologyauthors
López-García JS,García-Lozano I,Rivas L,Viso-Garrote M,Raposo R,Méndez MTdoi
10.1016/j.ajo.2020.01.027subject
Has Abstractpub_date
2020-05-01 00:00:00pages
260-266eissn
0002-9394issn
1879-1891pii
S0002-9394(20)30036-2journal_volume
213pub_type
杂志文章abstract：:A 46-year old woman and a 59-year-old man had corneal crystals, multiple myeloma, and IgG kappa hypergammaglobulinemia. In one case, crystalline deposits were also present in the lens. In both patients there was a marked decrease in the amount of crystals during chemotherapy. The crystals within the cornea of one case...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(78)90230-1
更新日期：1978-09-01 00:00:00
abstract：PURPOSE:To evaluate and compare the recovery of postoperative corneal sensitivity after laser in situ keratomileusis and photorefractive keratectomy for the correction of low myopia. METHODS:In a prospective study, 17 consecutive eyes (17 patients) underwent laser in situ keratomileusis to correct myopia ranging from ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(98)00444-9
更新日期：1999-05-01 00:00:00
abstract：:I fitted nine aphakic patients who had undergone penetrating keratoplasty with extended-wear hydrophilic contact lenses (55% to 80% water content). All patients had edematous host corneas. Eight of nine developed vascularization of the graft after two to 30 weeks of contact lens wear. One patient developed a presumed ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)74912-8
更新日期：1980-09-01 00:00:00
abstract：PURPOSE:To report the response of calcification in choroidal melanoma after transpupillary thermotherapy. METHODS:Interventional case report. A 66-year-old male with a posterior uveal melanoma measuring 10 x 8 x 4.4 mm underwent infrared diode laser transpupillary thermotherapy three times, each 12 weeks apart. For ea...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(01)01186-2
更新日期：2001-12-01 00:00:00
abstract：PURPOSE:To evaluate possible changes in macular morphology after cataract surgery with combined primary posterior capsulorhexis and posterior optic buttonholing in comparison to conventional in-the-bag intraocular lens (IOL) implantation. DESIGN:Prospective randomized study. METHODS:Fifty consecutive age-related cata...
journal_title：American journal of ophthalmology
pub_type： 杂志文章,随机对照试验
doi：10.1016/j.ajo.2008.02.022
更新日期：2008-07-01 00:00:00
abstract：PURPOSE:To report the association of antiretinal antibodies in patients with bilateral cystoid macular edema and retinitis pigmentosa. METHODS:In a prospective study, 30 consecutive patients with bilateral cystoid macular edema and retinitis pigmentosa were tested for antiretinal antibodies. As control subjects, 30 co...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(98)00446-2
更新日期：1999-05-01 00:00:00
abstract：PURPOSE:To evaluate the importance of nutritional supplements, dietary pattern, and genetic associations in age-related macular degeneration (AMD); and to discuss the technique of artificial intelligence/deep learning to potentially enhance research in detecting and classifying AMD. DESIGN:Retrospective literature rev...
journal_title：American journal of ophthalmology
pub_type：
doi：10.1016/j.ajo.2020.05.042
更新日期：2020-09-01 00:00:00
abstract：PURPOSE:We previously demonstrated that selective suture removal reduces keratoplasty astigmatism; however, a myopic shift was induced with the increasing number of interrupted sutures removed. This study is an attempt to determine the effects of a modified surgical technique on postkeratoplasty myopia, astigmatism, an...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(02)02287-0
更新日期：2003-06-01 00:00:00
abstract：:Nine patients with polyradiculomyeloneuropathy after acute hemorrhagic conjunctivitis (AHC) infection were studied serologically. All except one patient had neutralization titers larger than or equal to 1:16 against the prototype J670/71 strain of AHC virus in at least one of their serum samples. The development of a ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(75)90279-2
更新日期：1975-11-01 00:00:00
abstract：:We managed three cases of congenital iris stromal cysts and compared them with 22 cases in the published reports to delineate the clinical features of this condition and determine the best treatment. Our first patient, a 5-month-old girl, had injection of a cyst with trichloroacetic acid, but developed a cataract and ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章,评审
doi：10.1016/s0002-9394(14)71291-7
更新日期：1993-08-15 00:00:00
abstract：PURPOSE:We studied the incidence of vitreous hemorrhage in a well-defined population to determine the underlying causes of such hemorrhages. METHODS:We prospectively studied all eyes with dense spontaneous first-time vitreous hemorrhage during a 2 1/2-year period in the greater Göteborg area (542,000 inhabitants). Fol...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)71232-2
更新日期：1995-04-01 00:00:00
abstract：:An ultrastructural study of Bruch's membrane in 68 human eyes including at least five individuals from each of ten decades of life showed that changes began in the second decade in macular specimens but were delayed in nonmacular specimens. All specimens from 20-year-old donors had altered macular Bruch's membranes. T...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(85)90625-7
更新日期：1985-11-15 00:00:00
abstract：PURPOSE:To evaluate long-term outcomes after Ahmed valve implantation in patients with glaucoma when using adjunctive intraoperative mitomycin C (MMC) and postoperative 5-fluorouracil (5-FU). DESIGN:Retrospective, interventional, consecutive case series. METHODS:A consecutive series of eyes undergoing Ahmed valve imp...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2008.04.008
更新日期：2008-08-01 00:00:00
abstract：PURPOSE:To determine if optical coherence tomography (OCT) measurements of nerve fiber layer (NFL) thickness can be used to predict the presence of visual field defects (VFD) associated with glaucoma. DESIGN:Quota-sampled, cross-sectional study. METHODS:Retrospective study of OCT NFL thickness measurements in 276 eye...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(02)01683-5
更新日期：2002-10-01 00:00:00
abstract：:Normal volunteers used 1, 2, and 4% pilocarpine eyedrops, three times daily, or ocular therapeutic systems placed in the conjunctival cul-de-sac to control intraocular pressure. The systems continuously release 20 and 40 mug/hour of pilocarpine for one week. Although the amount of drug delivered to the eye from the o...
journal_title：American journal of ophthalmology
pub_type： 临床试验,杂志文章
doi：10.1016/0002-9394(75)90404-3
更新日期：1975-10-01 00:00:00
abstract：PURPOSE:Low docosahexaenoic acid (DHA) in X-linked retinitis pigmentosa (XLRP) may influence retinal function. The goals of this study were to elevate blood DHA levels and determine the effect on the rate of disease progression. DESIGN:In a 4-year prospective randomized clinical trial, male patients with XLRP (mean ag...
journal_title：American journal of ophthalmology
pub_type： 临床试验,杂志文章,随机对照试验
doi：10.1016/j.ajo.2003.10.045
更新日期：2004-04-01 00:00:00
abstract：PURPOSE:To report long-term clinical outcomes of deep anterior lamellar keratoplasty (DALK) in patients with keratoconus. DESIGN:Retrospective noncomparative interventional study. METHODS:setting: Single center. patients: Total of 158 eyes/150 consecutive patients with keratoconus with postoperative follow-up time eq...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2014.11.033
更新日期：2015-03-01 00:00:00
abstract：:Media opacification in eyes filled with silicone oil makes the evaluation of recurrent retinal detachment difficult. Ultrasonography through silicone oil is subject to significant imaging artifacts. We performed magnetic resonance imaging on six patients with unilateral intravitreal silicone oil to determine if the te...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)77016-3
更新日期：1990-10-15 00:00:00
abstract：PURPOSE:To report a rare presentation of the tubulointerstitial nephritis and uveitis syndrome. METHOD:Case report. A 34-year-old woman underwent extensive clinical and laboratory evaluation of bilateral intermediate uveitis and optic nerve edema. RESULTS:Laboratory evaluation and nephrology consultation led to a dia...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(98)00430-9
更新日期：1999-05-01 00:00:00
abstract：PURPOSE:To compare tear protein markers between normal subjects and patients with dry eye (DE) and high and low lymphotoxin-alpha (LT-α) levels. DESIGN:Prospective cross-sectional study. METHODS:Patients with DE were divided into low (≤700 pg/mL) and high (>700 pg/mL) LT-α groups. Twelve protein markers were measured...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2020.03.013
更新日期：2020-09-01 00:00:00
abstract：PURPOSE:To provide additional evidence that anterior uveitis can be a manifestation of poststreptococcal syndrome. METHOD:A case report providing follow-up information on a previously described patient. RESULTS:An adolescent girl in whom anterior uveitis was the only manifestation of poststreptococcal syndrome subseq...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：
更新日期：1999-03-01 00:00:00
abstract：:After penetrating keratoplasty, visual rehabilitation can be slow and is largely a function of corneal surface configuration. Computerized topographic analysis allows the detailed study of corneal surface factors that determine the optical function of the graft. We performed a prospective, longitudinal study of eight ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)73925-x
更新日期：1993-02-15 00:00:00
abstract：PURPOSE:To describe a technique for suturing a dislocated intraocular lens (IOL) using an intraocular cowhitch knot without IOL extraction. METHODS:Through the sideports of paracentesis, a cowhitch knot is made outside of the anterior chamber by looping 10-0 Prolene around the haptics of the IOL. The knot is then tied...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(00)00727-3
更新日期：2001-02-01 00:00:00
abstract：PURPOSE:To design and validate a vision-specific quality-of-life assessment tool to be used in a clinical setting to evaluate low-vision rehabilitation strategy and management. METHODS:Previous vision-related questionnaires were assessed by low-vision rehabilitation professionals and patients for relevance and coverag...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(00)00610-3
更新日期：2000-12-01 00:00:00
abstract：:Between 1976 and 1982, we examined 13 patients with clinical and radiographic findings consistent with optic nerve sheath meningioma. All patients had visual loss, evidence of optic nerve dysfunction, centrocecal visual field defects, and echographic and computed tomographic evidence of optic nerve or sheath enlargeme...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/0002-9394(85)90506-9
更新日期：1985-09-15 00:00:00
abstract：PURPOSE/METHODS:We observed a clinicopathologic correlation of a case of adenocarcinoma of the retinal pigment epithelium. RESULTS/CONCLUSIONS:The ultrasonographic findings of this tumor demonstrated a collar button profile with high internal reflectivity. The ultrasonographic characteristics in this case reflected th...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(14)73887-5
更新日期：1995-02-01 00:00:00
abstract：PURPOSE:To evaluate the outcome of surgeries for macular holes using trypan blue to facilitate delamination of the internal limiting membrane. DESIGN:A retrospective series of 18 patients. METHOD:All patients underwent a three-port pars plana vitrectomy with internal limiting membrane delamination using trypan blue 0...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(02)02239-0
更新日期：2003-06-01 00:00:00
abstract：PURPOSE:To determine whether central fusion and distance stereoacuity are useful as objective measures in assessing the need for and success of surgery for intermittent exotropia (X[T]). METHODS:A prospective, institutional, clinical trial was conducted of 26 consecutive patients with X(T) who were undergoing strabism...
journal_title：American journal of ophthalmology
pub_type： 临床试验,杂志文章
doi：10.1016/s0002-9394(99)00079-3
更新日期：1999-08-01 00:00:00
abstract：PURPOSE:To compare race-related differences in estimated rate of change of Bruch's membrane opening-minimum rim width (BMO-MRW) and circumpapillary retinal nerve fiber layer thickness (RNFLT) in healthy, glaucoma suspect, and glaucoma eyes of individuals of European (ED) and African descent (AD). DESIGN:Prospective co...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/j.ajo.2018.08.050
更新日期：2018-12-01 00:00:00
abstract：PURPOSE:To determine whether central corneal thickness (CCT) is a risk factor for visual field loss development among patients diagnosed with preperimetric glaucomatous optic neuropathy (GON). DESIGN:Observational cohort study. METHODS:The study included 98 eyes of 98 patients with GON, with a mean follow-up time of ...
journal_title：American journal of ophthalmology
pub_type： 杂志文章
doi：10.1016/s0002-9394(03)00484-7
更新日期：2003-11-01 00:00:00
